In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens

Blajman, Jesica E.; Gaziano, Cristian; Zbrun, María Virginia; Soto, L.P.; Astesana, Diego M.; Berisvil, Ayelén Patricia; Scharpen, Analía Romero; Signorini, Marcelo; Frizzo, L.S.

doi:10.1016/j.rvsc.2015.05.017

Cited by 41 publications

(34 citation statements)

References 36 publications

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“…Due to differences in breeding conditions, feed and water quality, and probiotic and CE culture type, confirmative results between scientific studies has not easily been demonstrated (Otutumi et al, 2012). Variations in the effects of probiotics on growth performance of broiler chickens may be attributed to differences in the strains of bacteria used as dietary supplements (Angel et al, 2005;Timmerman et al, 2006;O'Dea et al, 2006;Lutful Kabir, 2009;Blajman et al, 2015 andOlnood et al, 2015). Differences between physical and environmental conditions of the trials may also bias the results from these studies (Olnood et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Dietary Inclusion of Probiotics on Growth and Intestinal Morphology of Broiler Chickens

Gülmez¹,

Gülmez²,

Bingol³

et al. 2019

jwpr

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Probiotics are currently under investing the most valuable substances alternative to antibiotic growth promoters in poultry breeding practice. This research was performed to evaluate the effect of supplementing broiler drinking water with probiotics (Pediococcus acidilactici and Bacillus subtilis) at a concentration of ≥10 8 CFU/ml during 42 days of feeding period on growth performance and gut health. A total of 144 one-day-old Ross 308 broiler chicks (mixed gender) with an average initial BW of 42.3 g were used. The chicks were allotted to pens with 12 birds per pen and six replications per treatment with food and water provided ad libitum. Feed intake of Probiotic group was 4134 g, 338 g less than that of control group. Live weight of probiotic group was 2537 g and a 113 g more than that of control group. The feed conversion ratio of probiotic group was 1.61, 0.22 less than that of control group. The crypt depth of probiotic group (1110.46 ± 224.016 µm) was statistically deeper than that of control group (949.39 ± 114.166 µm) in ileum. Continuously use of probiotics in drinking water of commercial poultry flocks appears to be alternative to AGPs. The results of this study provide a greater understanding of the impact of long-life use of probiotics on broiler health and growth performances.

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Section: Discussionmentioning

confidence: 99%

The Effect of Dietary Inclusion of Probiotics on Growth and Intestinal Morphology of Broiler Chickens

Gülmez¹,

Gülmez²,

Bingol³

et al. 2019

jwpr

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“…En el presente estudio (tabla 2) se obtuvieron mejores resultados de ICA con los tratamientos que contenían el inóculo bacteriano, posiblemente debido a que este grupo bacteriano tiene influencia positiva sobre la digestibilidad de los alimentos, lo que conlleva a mejorar la conversión alimenticia (Guerra et al, 2007). Además, Blajman et al (2015) indican, además, que estas bacterias promueven el crecimiento e inhiben el desarrollo de microorganismos patógenos. Estudios similares de aplicación de inóculos bacterianos en animales de granja demuestran mejoras en ganancia de peso (Blajman et al, 2015).…”

Section: Resultados Y Discusiónunclassified

“…Además, Blajman et al (2015) indican, además, que estas bacterias promueven el crecimiento e inhiben el desarrollo de microorganismos patógenos. Estudios similares de aplicación de inóculos bacterianos en animales de granja demuestran mejoras en ganancia de peso (Blajman et al, 2015). Nota: a,b Superíndices diferentes dentro de columnas indican diferencia significativa entre tratamientos (p < 0,05).…”

Section: Resultados Y Discusiónunclassified

Feeding behavior of pre-weaned piglets dosed orally with bacteria of the digestive tract of the piglet

Sánchez¹,

Ochoa²,

Alfaro³

2017

SCIÉNDO

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Comportamiento alimenticio de lechones en predestete dosificados vía oral con inóculos bacterianos del tracto digestivo del lechónFeeding behavior of pre-weaned piglets dosed orally with bacteria of the digestive tract of the piglet RESUMENSe evaluó el efecto alimenticio de la administración oral de inóculos de bacterias ácido láctico (BAL) nativas, extraídos y aisladas del tracto digestivo de un lechón e 21 días de edad. Los inóculos evaluados fueron mezclas en proporciones iguales de Lactobacillus johnsonii, Lactobacillus brevis, Enterococcus hirae y Pediococcus pentosaceus y dosificadas en concentraciones obtenidas mediante densidad óptica de 1,5x10 8 , 3x10 8 y 4,5x10 8 UFC/ml, fue administrado vía oral a 36 lechones de 14 días de edad y una semana antes del destete (nueve lechones por tratamiento). No hubo diferencias significativas en ganancia diaria de peso; sin embargo, el índice de conversión alimenticia fue mejor en los tres tratamientos con inoculo (p < 0,05), específicamente el tratamiento que contenía 3x10 8 UFC/ml. Se concluye que los inóculos bacterianos nativos aplicados vía oral tuvieron un efecto positivo en la conversión alimenticia en el predestete de lechones.Palabras clave: bacterias ácido lácticas; inóculo bacteriano; bacterias nativas; destete. ABSTRACTThe nutritional effect of oral administration of inoculums of native lactic acid bacteria (BAL), extracted and isolated from the digestive tract of a piglet and 21 days of age, was evaluated. The evaluated inoculums were mixtures of Lactobacillus johnsonii, Lactobacillus brevis, Enterococcus hirae and Pediococcus pentosaceus in equal proportions and dosed at concentrations measured by optical density of 1,5x10 8 , 3x10 8 and 4,5x10 8 CFU / ml orally given to 36 piglet of 14 day and one week before weaning (nine piglets per treatment). There were no significant differences in daily weight gain; However, the feed conversion index was better in the three treatments with inoculum (p < 0.05), specifically the treatment containing 3x10 8 CFU / ml. It is concluded that native bacterial inoculums applied orally had a positive effect on feed conversion in the pre-weaned of piglets.

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“…LAB are described as part of a biosystem of apathogenic and pathogenic bacteria in equilibrium [ 43 ]. The contribution of LAB to maintain or restore a healthy vaginal flora in humans and animals has been the aim of an increasing number of research projects and is still not completely elucidated [ 32 , 33 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality

Schulze¹,

Schäfer²,

Simmet³

et al. 2018

PLoS ONE

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The presence of pathogenic bacteria in ejaculates has been a topic in boar semen preservation over the last decades. Since little information is available on commensal bacteria in boar semen, the aim of the present study was to identify commensal lactobacilli in fresh cryopreserved boar semen and to examine their influence on boar semen quality. Therefore, 111 boar ejaculates were investigated for the presence of Lactobacillus species. Thirty samples (27%) contained viable Lactobacillus species (e.g. L. amylovorus, L. animalis, L. reuteri and Weisella minor). L. animalis and L. buchneri DSM 32407 (isolated from the bovine uterus) qualified for further examinations based on their growth rate in six antibiotic-free boar semen extenders. After a 120 min short-term incubation with an antibiotic-free BTS-extender, progressive motility was diminished (P = 0.001) upon addition of 105 and 106 colony forming units (CFU/mL) L. animalis. The supplementation with L. buchneri DSM 32407 had no significant (P > 0.05) influence on sperm quality during short-term co-incubation. After 168 h long-term co-incubation, motility analysis revealed a negative (P = 0.026) impact of 105 CFU/mL L. buchneri DSM 32407. A concentration- and storage-dependent effect is particularly obvious (P < 0.001) using 106 CFU/mL L. buchneri DSM 32407. Most notably, the thermo-resistance (TRT) for 106 CFU/mL L. buchneri DSM 32407 (P = 0.001) was inferior to BTS with and without gentamicin after 72 and 168 h of semen co-incubation. The supplementation of 105 CFU/mL L. buchneri DSM 32407 impaired progressive motility to a lesser extent. The percentage of mitochondrially active spermatozoa after 96 h (P = 0.009) and membrane-intact spermatozoa after 168 h (P < 0.001) was lower when 106 CFU/mL L. buchneri DSM 32407 were suspended compared with all other groups. Finally, the addition of L. buchneri DSM 32407 to BTS-extended boar semen had no competitive effect on the total amount of bacteria 48 h after co-incubation. In summary, the present study demonstrated that there are Lactobacillus species present in the porcine seminal plasma, which can be cultivated using standard procedures. However, long-term co-incubation of lactic acid bacteria with spermatozoa had a negative influence on spermatozoa.

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In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens

Cited by 41 publications

References 36 publications

The Effect of Dietary Inclusion of Probiotics on Growth and Intestinal Morphology of Broiler Chickens

The Effect of Dietary Inclusion of Probiotics on Growth and Intestinal Morphology of Broiler Chickens

Feeding behavior of pre-weaned piglets dosed orally with bacteria of the digestive tract of the piglet

Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality

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