In vitro and in vivo evaluation of electrospun nanofibers of PCL, chitosan and gelatin: A comparative study

Gomes, Susana R.; Rodrigues, Gabriela; Martins, Gabriel G.; Roberto, Maria Angélica; Mafra, Manuela; Henriques, Célia; Silva, Jorge

doi:10.1016/j.msec.2014.10.051

Cited by 224 publications

(96 citation statements)

References 48 publications

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“…Electrospinning of pure chitosan solution leads to the formation of beads instead of fine nanofibers. Then, taking into account data from literature [10,17,18,35,36,42], it was decided to mix chitosan with PEO, allowing for better electrospinning.…”

Section: Resultsmentioning

confidence: 99%

“…The use of ionic liquid was also mentioned with 1,1,1,3,3,3-hexafluoro-2-propanol [28]. More generally, it has been shown that fine nanofibers can be obtained in chitosan blends with poly (vinyl alcohol) (PVA) [23,25,29,30], gelatin or collagen [2,7,31,32,33,34], silk fibroin [5], polycaprolactone [35] but mainly with poly(ethylene oxide) (PEO) [6,17,18,36,37,38,39,40,41]. Usually, to generate composite fibers the spinning solutions are obtained by mixing the two polymers solutions prepared separately in the same solvent.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly(ethylene oxide)

Lemma

Bossard

Rinaudo³

2016

IJMS

102

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Electrospinning was employed to obtain chitosan nanofibers from blends of chitosans (CS) and poly(ethylene oxide) (PEO). Blends of chitosan (MW (weight-average molecular weight) = 102 kg/mol) and PEO (M (molecular weight) = 1000 kg/mol) were selected to optimize the electrospinning process parameters. The PEO powder was solubilized into chitosan solution at different weight ratios in 0.5 M acetic acid. The physicochemical changes of the nanofibers were determined by scanning electron microscopy (SEM), swelling capacity, and nuclear magnetic resonance (NMR) spectroscopy. For stabilization, the produced nanofibers were neutralized with K2CO3 in water or 70% ethanol/30% water as solvent. Subsequently, repeated washings with pure water were performed to extract PEO, potassium acetate and carbonate salts formed in the course of chitosan nanofiber purification. The increase of PEO content in the blend from 20 to 40 w% exhibited bead-free fibers with average diameters 85 ± 19 and 147 ± 28 nm, respectively. Their NMR analysis proved that PEO and the salts were nearly completely removed from the nanostructure of chitosan, demonstrating that the adopted strategy is successful for producing pure chitosan nanofibers. In addition, the nanofibers obtained after neutralization in ethanol-aqueous solution has better structural stability, at least for six months in aqueous solutions (phosphate buffer (PBS) or water).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly(ethylene oxide)

Lemma

Bossard

Rinaudo³

2016

IJMS

102

View full text Add to dashboard Cite

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“…However, such scaffolds are usually isotropic and provide no guidance cues for cells. In this study we focused on PCL fiber scaffolds produced through electrospinning, since these have been thoroughly evaluated for cell culturing and tissue engineering purposes [23]. PCL has a low degradation rate (many months in vivo and even longer in vitro )[24] and high elongation parameters, both necessary properties for clinical grafts and implants, where the material is required to be stable over sufficient time, but still enables bending, contraction and expansion [25].…”

Section: Discussionmentioning

confidence: 99%

Accelerated Wound Closure - Differently Organized Nanofibers Affect Cell Migration and Hence the Closure of Artificial Wounds in a Cell Based In Vitro Model

2017

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Nanofiber meshes holds great promise in wound healing applications by mimicking the topography of extracellular matrix, hence providing guidance for crucial cells involved in the regenerative processes. Here we explored the influence of nanofiber alignment on fibroblast behavior in a novel in vitro wound model. The model included electrospun poly-ε-caprolactone scaffolds with different nanofiber orientation. Fibroblasts were cultured to confluency for 24h before custom-made inserts were removed, creating cell-free zones serving as artificial wounds. Cell migration into these wounds was evaluated at 0-, 48- and 96h. Cell morphological analysis was performed using nuclei- and cytoskeleton stainings. Cell viability was assessed using a biochemical assay. This study demonstrates a novel in vitro wound assay, for exploring of the impact of nanofibers on wound healing. Additionally we show that it’s possible to affect the process of wound closure in a spatial manner using nanotopographies, resulting in faster closure on aligned fiber substrates.

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“…This property, along with good compatibility and easy processing (melting point at 60°C), makes PCL an interesting substrate for tissue engineering (20)(21)(22)(23)(24)(25). However, like other synthetic polymers, PCL also lacks surface wettability and functional surface groups improving the cell attachment that are essential in tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering

et al. 2016

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Abstract. In this study, biodegradable poly(ε-caprolactone) (PCL) nanofibers (PCL-NF), collagen-coated PCL nanofibers (Col-c-PCL), and titanium dioxide-incorporated PCL (TiO 2 -i-PCL) nanofibers were prepared by electrospinning technique to study the surface and structural compatibility of these scaffolds for skin tisuue engineering. Collagen coating over the PCL nanofibers was done by electrospinning process. Morphology of PCL nanofibers in electrospinning was investigated at different voltages and at different concentrations of PCL. The morphology, interaction between different materials, surface property, and presence of TiO 2 were studied by scanning electron microscopy (SEM), Fourier transform IR spectroscopy (FTIR), contact angle measurement, energy dispersion X-ray spectroscopy (EDX), and Xray photoelectron spectroscopy (XPS). MTT assay and cell adhesion study were done to check biocompatibilty of these scaffolds. SEM study confirmed the formation of nanofibers without beads. FTIR proved presence of collagen on PCL scaffold, and contact angle study showed increment of hydrophilicity of Col-c-PCL and TiO 2 -i-PCL due to collagen coating and incorporation of TiO 2 , respectively. EDX and XPS studies revealed distribution of entrapped TiO 2 at molecular level. MTT assay and cell adhesion study using L929 fibroblast cell line proved viability of cells with attachment of fibroblasts over the scaffold. Thus, in a nutshell, we can conclude from the outcomes of our investigational works that such composite can be considered as a tissue engineered construct for skin wound healing.

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In vitro and in vivo evaluation of electrospun nanofibers of PCL, chitosan and gelatin: A comparative study

Cited by 224 publications

References 48 publications

Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly(ethylene oxide)

Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly(ethylene oxide)

Accelerated Wound Closure - Differently Organized Nanofibers Affect Cell Migration and Hence the Closure of Artificial Wounds in a Cell Based In Vitro Model

Structural and Surface Compatibility Study of Modified Electrospun Poly(ε-caprolactone) (PCL) Composites for Skin Tissue Engineering

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