In vitro androgenic response of minipaprika (Capsicum annuum L.) genotypes in different culture media

In vitro Androgenesis in Capsicum (Capsicum annuum L.)

“…These results, i.e. genotype dependence on androgenic response are in consistent with the reports of several earlier workers (Dumas de Vaulx et al, 1981;Rodeva et al, 2004;Lantos et al, 2009;Zhao et al, 2010;Niklas et al, 2012;Liljana et al, 2013;Luitel andKang, 2013 andOlszewska et al, 2014).…”

“…Beneficial effect of auxin and cytokinins added to the culture media on haploid callus and embryo induction was reported by ; Supena and Custers (2011);Lantos et al, (2012) ;Luitel and Kang (2013) and Olszewska et al, (2014). In both the hybrids, the percentage of callusing anthers was significantly higher on medium supplemented with zeatin 0.5 at mg/l along with 2, 4-D at 0.2 mg/l and AgNO 3 at 15.0 mg/l (Plate 2b&c).…”

“…Taskin et al, (2011) obtained embryos from anthers after heat pretreatment at 35°C. Similarly, Luitel and Kang (2013) cultured pepper anthers and kept them in dark at 35°C for seven days. Although the mode of action is not clear, a thermal treatment seems to activate the anthers for androgenesis.…”

Morphological characteristics of doubled haploid plants of pepper produced using microspore/anther in vitro culture of the interspecies hybrids of Capsicum annum L. and C. chinense Jacq

“…It is possible to assume that the genotypes able to synthesize capsaicin are more responsive to the induction of embryogenesis from microspores. Historically, the most efficient DH technologies initially were developed for the varieties of hot pepper, apparently more responsive to the induction of androgenesis [5,6], and then for the sweet pepper varieties [7,8]. These technologies are intended for the varieties of both sweet and hot C. annuum, and the number of studies of producing the DH and H plants for other species of pepper are limited.…”

Embryogenesis in the anthers of different ornamental pepper (Capsicum annuum L.) genotypes

“…However, the presence of embryos was not verified (only calli were present), so embryogenesis induction was only achieved when these anthers were cultured in C medium. Luitel and Kang (2013) also obtained inferior results in three genotypes of minipaprika (C. annuum L.) cultured in MS medium as compared to C medium. These results differ from the findings of this experiment where a larger quantity of embryos was found in MS media corresponding to M1 and M2 media.…”