In vitro angiotensin-converting enzyme and dipeptidyl peptidase-IV inhibitory, and antioxidant activity of blue mussel (Mytilus edulis) byssus collagen hydrolysates

Abstract

Large quantities of mussel byssus are generated annually as a co-product of the mussel-processing industry. This fibrous material is a rich source of collagen, which when extracted has potential uses as an alternative source of collagen for food applications. However, due the complex structure of the material, the extraction of the collagenous components using food-friendly strategies has proved challenging to date. An enzyme-aided method, using a proline endoproteinase, was employed for the extraction of collagen from mussel byssus yielding 138.82 ± 2.25 mg collagen/g dry weight. Hydrolysates of the collagen extract were generated using five food-grade enzyme preparations with Corolase® PP giving the highest extent of hydrolysis. Reversed-phase and gel permeation high-performance liquid chromatography of the extracted collagen and its enzymatic hydrolysates showed significant hydrolysis of collagen. The hydrolysates generated with Corolase® PP showed the highest in vitro bioactivities: angiotensin-converting enzyme (ACE) IC50 = 0.79 ± 0.17 mg/ml, dipeptidyl peptidase-IV (DPP-IV) IC50 = 0.66 ± 0.17 mg/ml and oxygen radical absorbance capacity (ORAC) activity = 311.23 ± 13.41 µmol trolox equivalents (TE)/g. The results presented herein indicate that in addition to acting as an alternative source of collagen for food applications, mussel byssus collagen-derived hydrolysates have potential applications as functional food ingredients for the management of metabolic diseases such as type II diabetes and hypertension.

Marine-Derived Peptides with Anti-Hypertensive Properties: Prospects for Pharmaceuticals, Supplements, and Functional Food

“…The blue mussel industry generates substantial amounts of by-products beyond these non-saleable co-products. Hydrolysis of blue mussel byssus, a collagenous protein strand, with corolase PP displayed ACE inhibitory activity higher than that of unhydrolyzed protein [29]. The processing of bivalves for food generates substantial quantities of shell by-products.…”

Developments for collagen hydrolysates as a multifunctional antioxidant in biomedical domains

“…Nrf2 is the most important regulator of cellular antioxidant activity and mediates antioxidant responses to inflammation and oxidative stress [27]. Another study showed that the antioxidant collagen hydrolysate from blue mussel byssus exhibited the highest ACE inhibition potencies, thereby having the potential for the management of cardiovascular diseases [111]. Alemán et al [48] demonstrated the presence of Leu residues in the antioxidant collagen peptide sequence (GPLGLLGFLGPLGLS), which appears to play an important role in their ACEinhibitory activity.…”