In vitro antagonistic potential, plant growth-promoting activity and indole-3-acetic acid producing trait of bacterial isolates from spent mushroom substrate of Agaricus bisporus

Abstract

Spent mushroom substrate (SMS) is widely used as fertilizer and to control plant diseases. The microorganisms surviving in SMS play a crucial role in plant growth promotion and biocontrol properties of SMS. In this study, an effort was made to isolate and characterize the bacterial species present in the SMS of Agaricus bisporus and to study their antagonistic potential, plant growth-promoting ability and indole-3-acetic acid (IAA) producing trait. Six different bacterial isolates exhibiting morphological variabilities were obtained from the SMS by serial dilution technique. On the basis of 16S rRNA gene sequences, these isolates were identified as Staphylococcus epidermidis (Sh1 and Sh3), S. aureus (Sh2), Bacillus albus (Sh4), Delftia lacustris (Sh6) and Comamonas aquatica (Sh7). These bacterial strains were assayed for their antagonism against Pythium aphanidermatum, a phytopathogenic oomycete. The results of in vitro dual culture assay revealed that all the 6 bacterial isolates showed low levels of suppression of P. aphanidermatum and recorded less than 5 mm inhibition zone. Among the bacterial isolates, S. epidermidis Sh3 recorded the maximum inhibition zone of 4.2 mm. Plant growth promotion test using roll paper towel method revealed that C. aquatica Sh7, B. albus Sh4, D. lacustris Sh6 and S. epidermidis Sh3 caused a significant increase in seedling vigour of cucumber compared to control. The seeds treated with the bacterial isolate C. aquatica Sh7 showed the maximum seedling vigor. Assessment of in vitro production of IAA by the bacterial isolates revealed that the bacterial isolates highly varied (ranging from 0.28 to 9.25 mg L-1) in their potential for production of IAA. The maximum amount of IAA was produced by C. aquatica Sh7 (9.25 mg L-1), while the minimum was produced by S. epidermidis Sh1 (0.28 mg L-1).

The BIOHERBICIDAL ACTIVITY OF Pseudomonas SPP (PF-097) To SUPRESS THE ALLELOPATHIC STRESS OF Trianthema portulacastrum LEAF EXTRACT ON Vigna mungo

“…They produce high amount of physiologically active gibberellins and enhance stem elongation. PGPR also enhance the endogenous indole-3-acetic acid content and development of Vigna radiata bacterial strains of Micrococcus, Bacillus, Escherichia, Pseudomonas and Staphylococcus improve indole-3acetic acid (IAA) content and growth of wheat (Velazhahan et al, 2020). PGPR increased the number of tillers, shoot length, spike length, and seed weight.…”

Spent substrate from mushroom cultivation: exploitation potential toward various applications and value-added products

“…The bioactive compounds in SMS have antimicrobial properties [ 132 ], which could be exploited against plant pathogens. Although in vitro studies have shown the potential suitability of mushroom and mycelium extracts against plant pathogens [ 87 , 133 , 134 ], they do not necessarily reveal the in vivo effectiveness. SMS application has shown to be effective in suppressing plant disease incidence.…”