In vitro anthelmintic activity of Pimienta dioica and Origanum vulgare essential oils on gastrointestinal nematodes from sheep and cattle

Jiménez-Penago, Gerardo; González-Gardúño, Roberto; Martínez-Bolaños, Luciano; Maldonado‐Simán, Ema; Cruz-Tamayo, Alvar A.; Gives, Pedro Mendoza de

doi:10.1007/s12639-021-01401-1

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…In our first study, the EO of oregano showed a maximal inhibitory effect (100%) on the egg hatch inhibition for concentrations ranging from 0.049 to 50 mg/mL [3]. In a study by Jimenez-Penago et al [45], the EO of O. vulgare showed a similar effect against sheep GINs (H. contortus) as in the present study, with a concentration needed to reduce 50% hatching of 0.17-0.28 mg/mL, although the chemical composition of that sample was different (consists mainly from eugenol, 76.3%). In the same study, the EO also showed high activity against GINs isolated from cattle (Cooperia spp.…”

Section: Discussionmentioning

confidence: 66%

A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep

Štrbac

Krnjajić

Maurelli

et al. 2022

Animals

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The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of O. vulgare was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.

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Section: Discussionmentioning

confidence: 66%

A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep

Štrbac

Krnjajić

Maurelli

et al. 2022

Animals

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“…The use of essential oils (EOs) offers another alternative for the control of GIN, and it has been especially tried in sheep [37]. Several studies investigated the activity of EOs against ovine GIN by means of egg hatch tests [19,36] or through the larval development test [10]. In addition, some in vivo studies have been carried out on sheep [37].…”

Section: Introductionmentioning

confidence: 99%

Preliminary screening of plant essential oils from an oceanic climate zone (NW Spain) for the control of equine cyathostomins

Garduño,

Vázquez,

Monteiro

et al. 2024

Discov Appl Sci

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Cyathostomins (Strongylida: Cyathostominae) are gastrointestinal nematodes (GIN) that affect equines in many parts of the world. Due to anthelmintic resistance (AR), it is necessary to search for control alternatives. The objective of the study was to carry out a screening of 26 essential oils (EOs) from plants to determine in vitro their action on cyathostomins. Essential oils were obtained by hydro-distillation from leaves, flowers, fruits, and seeds of the selected species and used against the eggs of cyathostomins by means of the tests of egg hatching and larval motility inhibition. For each EO, different concentrations were prepared (250, 125, 62.5, 31.3, 15.6, 7.8, 3.9, 2.0, 1.0, 0.5 and 0.24 mg/mL). Oxfendazole at 2.5% mixed with closantel 5% was used as positive control, and PBS served as negative control. Cyathostomin eggs were obtained from feces of infected horses and infective larvae were recovered after coproculture. The in vitro test was carried out 36 h after the challenge with the EOs. The highest production of EOs was obtained from leaves of bay, eucalyptus, mallow, and basil, as well as from seeds of black pepper, coriander, cinnamon, anise, and peel of orange and cloves, cumin, and Lawson’s cypress. The oils extracted from laurel leaves, ground cinnamon, anise, cumin, and coriander seeds had a very high effect on cyathostomins eggs up to concentrations lower than 3.9 mg/mL. Therefore, in addition to the uses against other pathogens such as fungi and bacteria, some EOs might attain great importance as an alternative control strategy in the control of nematode eggs.

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Nematophagous fungi, an extraordinary tool for controlling ruminant parasitic nematodes and other biotechnological applications

Gives

Braga

Araújo

2022

Biocontrol Science and Technology

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In vitro anthelmintic activity of Pimienta dioica and Origanum vulgare essential oils on gastrointestinal nematodes from sheep and cattle

Cited by 4 publications

References 31 publications

A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep

A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep

Preliminary screening of plant essential oils from an oceanic climate zone (NW Spain) for the control of equine cyathostomins

Nematophagous fungi, an extraordinary tool for controlling ruminant parasitic nematodes and other biotechnological applications

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