In vitro anti-amylase activity of some Indian dietary spices

Abstract

Pancreatic alpha amylase (PAA) inhibitory activity of cinnamon, cumin was evaluated in vitro to search new anti-diabetic agents as alternatives to synthetic medicines. Bark of cinnamon, seeds of cumin, fenugreek, nutmeg, fennel, and buds of clove were extracted with hot water, methanol, chloroform, benzene, and ethyl acetate and 30 extracts were tested for the presence of PAA inhibitory activity using qualitative and quantitative methods and their modes of inhibition were determined. Presence of alpha amylase inhibitors was identified in 18 extracts in quantitative assay. Benzene extracts of cinnamon, clove, fenugreek, and nutmeg and chloroform extract of cumin showed highest anti-amylase potential. The IC50 values of these potential extracts ranged between 2.09 ± 0.12 mg/ml and 2.89 ± 0.079 mg/ml with lowest IC50 value noted for benzene extract of cinnamon and highest IC50 value was noted for benzene extract of fenugreek. Based on the values of Vmax and Km compared to control, it was found that all these extracts display non-competitive mode of inhibition on amylase activity. In conclusion, active constituents of these five extracts possess anti-amylase properties and can be used in management of diabetes mediated complications.

HPLC-DAD-MS Characterization, Antioxidant Activity, α-amylase Inhibition, Molecular Docking, and ADMET of Flavonoids from Fenugreek Seeds

“…However, [87] findings were found to be in approximate agreement with our findings, in which the IC 50 value was 690 ± 0.01 µg/mL, whereas [88] did not notice any effect of methanol extract on the concentration of 2.5 mg/mL, in which IC 50 value used to inhibit α-amylase of aqueous, hydroalcohol, and ethanol extracts 6.476, 12.395, and 8.690 mg/mL, respectively [89]. Also, the inhibition ratio in the concentration of 1 mg/mL was 37.7% [90], whereas in the [91] research study, the α-amylase inhibition ratio was 41.64%.…”

Bazi Baharatlarin Anti̇di̇yabeti̇k Etki̇leri̇;genel Bakiş

“…Kimyonun anti-diyabetik etkisi; pankreas beta hücrelerin varlığını korumak, insülin sekresyonu ve glikojen depolarını artırması ile ilişkili olabileceği düşünülmektedir [50]. Ayrıca kimyon alfa amilaz enziminin inhibisyonunu sağlayarak karbonhidrat sindirimini geciktirmektedir [51,52]. Srivsatava…”