In Vitro Anti-Inflammatory, Anti-Oxidant, and Cytotoxic Activities of Four Curcuma Species and the Isolation of Compounds from Curcuma aromatica Rhizome

Pintatum, Aknarin; Maneerat, Wisanu; Logie, Emilie; Tuenter, Emmy; Sakavitsi, Maria Eleni; Pieters, Luc; Berghe, Wim Vanden; Sripisut, Tawanun; Deachathai, Suwanna; Laphookhieo, Surat

doi:10.3390/biom10050799

Cited by 43 publications

(26 citation statements)

References 38 publications

(32 reference statements)

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“…Major chemical constituents found in C. aromatica were volatile terpenes, sesquiterpenes, and curcuminoids. ese compounds may react with the chemicals used in the total flavonoid assay leading to the overestimation of the values [21]. C. verum also showed high content of flavonoids, the major components being cinnamaldehyde and methyl eugenol [22].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Cytotoxic Activity against Breast, Cervical, and Ovarian Cancer Cells and Flavonoid Content of Plant Ingredients Used in a Selected Thai Traditional Cancer Remedy: Correlation and Hierarchical Cluster Analysis

Tuy-on

Itharat

Maki

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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This study aimed to investigate in vitro cytotoxic activity of selected plant ingredients from a traditional Thai remedy for the treatment of cancer patients against cancer cells occurring in women such as MCF-7 (breast cancer), SKOV3 (ovarian cancer), and HeLa (cervical cancer) cell lines. The plants and the remedy were macerated with 95% ethanol and boiled in water. Cytotoxic activity of the extracts was analyzed by SRB assay. Total flavonoid contents of the extracts were determined and their correlation with cytotoxic activity was evaluated. The hierarchical cluster analysis (HCA) was used to classify the extracts by their cytotoxic characteristics. A total of 66.7% of the plants was active against the tested cancer cell lines. Among the 44 plants in the remedy used for cancer treatment, nine plants that are also used in Thai cuisine exerted significant cytotoxicity against tested cancer cell lines. Eleven plants in the remedy were active against at least one of the tested cancer cell lines. All extracts were grouped into three groups and illustrated as heat map and hierarchical dendrogram. Total flavonoid content showed weak or no correlation with cytotoxic activity. A. dahurica, F. albopurpurea, and T. indica selectively exerted potent cytotoxic activity against MCF-7 with SI value more than 6. A. galanga, P. amarus, L. striatum, H. indicum, and F. vulgare exerted moderate cytotoxicity to all tested cell with low toxicity to normal cells. The correlation and HCA performed in this study provided an alternative way to investigate biological activities of plant ingredients in polyherbal traditional remedies.

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Section: Discussionmentioning

confidence: 99%

In Vitro Cytotoxic Activity against Breast, Cervical, and Ovarian Cancer Cells and Flavonoid Content of Plant Ingredients Used in a Selected Thai Traditional Cancer Remedy: Correlation and Hierarchical Cluster Analysis

Tuy-on

Itharat

Maki

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…including anti-inflammatory, hepatoprotective, neuroprotective and antioxidant activities (15,16). Thus, curcumenol may be considered a potential option for treating inflammation.…”

Section: Curcumenol Mitigates Chondrocyte Inflammation By Inhibiting the Nf-κb And Mapk Pathways And Ameliorates Dmm-induced Oa In Micementioning

confidence: 99%

Curcumenol mitigates chondrocyte inflammation by inhibiting the NF‑κB and MAPK pathways, and ameliorates DMM‑induced OA in mice

et al. 2021

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At present, an increasing number of individuals are affected by osteoarthritis (OA), resulting in a heavy socioeconomic burden. OA in knee joints is caused by the release of inflammatory cytokines and subsequent biomechanical and structural deterioration. To determine its anti-inflammatory function, the current study investigated the use of the plant-derived medicine, curcumenol, in OA treatment. curcumenol was not cytotoxic to ATdc5 chondrocytes and primary chondrocytes, as determined using a cell viability test. When these cells were treated with TNF-α and IL-1β to induce inflammation, curcumenol treatment inhibited the progression of inflammation by inactivating the NF-κB and MAPK signaling pathways, as well as decreasing the expression levels of MMP3 (as indicated by reverse transcription-quantitative PcR and western blotting). Moreover, to analyze metabolic and catabolic status in high-density and pellet culture, catalytic changes and the degradation of the extracellular matrix induced by TNF-α and IL-1β, were evaluated by alcian blue staining. These catalytic deteriorations were ameliorated by curcumenol. Using curcumenol in disease management, the mechanical and metabolic disruption of cartilage caused in the destabilization of medial meniscus (dMM) model was prevented in vivo. Thus, curcumenol mitigated inflammation in ATdc5 chondrocytes and primary mice chondrocytes, and also ameliorated OA in a dMM-induced mouse model.

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“…20 Among the WWWTC prescription, the Cmnamomi Mmulus and Epimedii Folium, Rhizoma Wenyujin Concisum, Poria, and Scutellariae Radix were the Monarch, Minister, Assistant, and Guide drug, respectively. Many compounds in WWWTC displayed a positive effect on anti-inflammatory, immuno-enhancement, and autophagy activity including coumarin (46), 58 cinnamic acid (64), 59 cinnamaldehyde (68), 59 cinnamic alcohol (72), 59 and 2-methoxycinnamaldehyde(73) 60 derived from Monarch medicine of Cmnamomi Mmulus; epimedin C (60), 61 icariin (62), 61 baohuoside I (82), 62 and anhydroicaritin (87) 63 derived from Monarch medicine Epimedii Folium; germacrone (88), 64 the sesquiterpene compound in Minister medicine Rhizoma Wenyujin Concisum and pachymic acid (90), 65 the tetracyclic triterpenoid acids in Assistant medicine Poria; baicalin (53), 66 baicalein (71), 66 wogonin (81), 66 and wogonoside (66) 66 in Guide medicine Scutellariae Radix, respectively. Therefore, according to the theory of Q-marker proposed by Liu et al, 5,6 the 15 Q-markers in this study were selected out, and the contents of them in WWWTC were determined.…”

Section: Selection Of Q-markersmentioning

confidence: 99%

Screening the Q‐markers of TCMs from RA rat plasma using UHPLC‐QTOF/MS technique for the comprehensive evaluation of Wu‐Wei‐Wen‐Tong Capsule

et al. 2021

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The appropriate selection of quality marker (Q‐marker) for performing the comprehensive quality evaluation of traditional Chinese medicines (TCMs) has much more significance. Wu‐Wei‐Wen‐Tong Capsule (WWWTC), a TCMs prescription, is mainly utilized to treat rheumatoid arthritis (RA) in China. However, the comprehensive quality control for WWWTC has not been achieved because of lacking system analysis for the Q‐marker. In this study, a dual wavelength, 203 and 270 nm, was selected based on the feature of 15 Q‐markers, and a reliable UHPLC‐UV fingerprinting approach was established, achieving the comprehensive quality evaluation of WWWTC. First, we identified 91 prototypes in rat plasma after administering a set amount of WWWTC by using UHPLC‐QTOF/MS technique and selected them as the candidate Q‐markers. Next, based on the “five principles” of Q‐marker selection, 15 absorbed components among them including coumarin, cinnamic acid, cinnamaldehyde, cinnamic alcohol, and 2‐methoxycinnamaldehyde derived from Monarch medicine of Cmnamomi Mmulus; epimedin C, icariin, baohuoside I, and anhydroicaritin derived from Monarch medicine Epimedii Folium; germacrone, the sesquiterpene compound in Minister medicine Rhizoma Wenyujin Concisum; pachymic acid, the tetracyclic triterpenoid acids in Assistant medicine Poria; baicalin, baicalein, wogonin, and wogonoside in Guide medicine Scutellariae Radix, respectively, were seriously chosen as the Q‐markers, indicating preferable pharmacological effect on RA, characterization of transitivity and traceability as well as measurable components in WWWTC. The effective and meaningful strategy displayed a unique perspective for the exploration of Q‐markers in the quality evaluation and further ensured efficacy and safety of the TCMs.

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In Vitro Anti-Inflammatory, Anti-Oxidant, and Cytotoxic Activities of Four Curcuma Species and the Isolation of Compounds from Curcuma aromatica Rhizome

Cited by 43 publications

References 38 publications

In Vitro Cytotoxic Activity against Breast, Cervical, and Ovarian Cancer Cells and Flavonoid Content of Plant Ingredients Used in a Selected Thai Traditional Cancer Remedy: Correlation and Hierarchical Cluster Analysis

In Vitro Cytotoxic Activity against Breast, Cervical, and Ovarian Cancer Cells and Flavonoid Content of Plant Ingredients Used in a Selected Thai Traditional Cancer Remedy: Correlation and Hierarchical Cluster Analysis

Curcumenol mitigates chondrocyte inflammation by inhibiting the NF‑κB and MAPK pathways, and ameliorates DMM‑induced OA in mice

Screening the Q‐markers of TCMs from RA rat plasma using UHPLC‐QTOF/MS technique for the comprehensive evaluation of Wu‐Wei‐Wen‐Tong Capsule

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