In vitro anti-microbial and in vivo cytokine modulating effects of different prepared Chinese herbal medicines

Lin, Shyh‐Jye; Chen, Chung-Shih; Lin, Shuwen; Chou, Ming‐Yung; Shih, Hung-Che; Lee, I-Pang; Kao, Chia‐Tze; Ho, Chuan-Chen; Chen, Fong-Lin; Ho, Yung‐Chuan; Hsieh, Kuanghui; Huang, Chi‐Ruei; Yang, Chi-Chiang

doi:10.1016/j.fct.2006.07.010

Cited by 23 publications

(17 citation statements)

References 31 publications

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“…However, certain cases of periodontitis may require additional antimicrobial agents in order to eliminate all the pathogens in the infected site (17). Chlorhexidine, antibiotics, and enzyme complexes are among the most frequently used antimicrobial agents, nevertheless, they are associated with certain disadvantages which limit their long-term application in cases of chronic periodontitis (13).…”

Section: Discussionmentioning

confidence: 99%

Assessing the Effect of Pineapple Extract Alone and in Combination With Vancomycin on Streptococcus sanguis

Khosropanah¹,

Bazargani²,

Ebrahimi³

et al. 2012

Jundishapur J Nat Pharm Prod

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Background: Periodontitis is a major problem that affects a large number of patients in the society. Various treatment alternatives have been proposed to control this pathologic condition. Streptococcus sanguis is one of the countless pathogens involved in periodontitis. Objectives: The current study aimed to assess the efficacy of pineapple extract per se and in synergy with vancomycin on the growth activity of S. Sanguis. Materials and Methods: An experimental study was designed to determine the minimum inhibitory concentration (MIC) of Pineapple extract per se and in conjunction with vancomycin. The study was carried out in three stages. Serial concentrations of the aqueous pineapple extract, vancomycin, and pineapple plus vancomycin were prepared by broth microdilution technique respectively and were exposed to the standard laboratory strain of S. sanguis (10556 ATCC). The lowest concentration of the pineapple extract and the mixed pineapple/vancomycin solution which inhibited bacterial growth was recorded as the MIC. Results:The minimum inhibitory concentration of vancomycin was determined 1 µg/ ml. The pineapple extract failed to show any inhibitory effects per se, however, once added to vancomycin, it reduced the MIC to 0.5µg/ml. Conclusion: Prescription of pineapple extract along with antibiotics increases the antibacterial effects of the drug, therefore reduces the minimum inhibitory concentration of the antibiotic.

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Section: Discussionmentioning

confidence: 99%

Assessing the Effect of Pineapple Extract Alone and in Combination With Vancomycin on Streptococcus sanguis

Khosropanah¹,

Bazargani²,

Ebrahimi³

et al. 2012

Jundishapur J Nat Pharm Prod

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“…In recent years, rather active investigations of its anti-tumor actvity were performed and this effect was from the improving immune functions (52). More recently, Ching-Wei-San and its individual herbal components, Coptidis rhizoma, Angelicae sinensis radix, Rehmanniae radixet rhizom, Moutan radicis cortex, and Cimicifuga foetida, were tested in our lab for in vitro inhibitory effects on three well-known plaque-causing bacteria, Porphyromonas gingivialis, Streptococcus sanguis, and Streptococcus mutans, and two common pathogens, Staphylococcus aureus and Escherichia coli (53). The cytokine modulating effects were also evaluated in BALB/c mice.…”

Section: Anti-microbe and Anti-cancer Effectsmentioning

confidence: 99%

The Immunopharmaceutical Effects and Mechanisms of Herb Medicine

Huang¹,

Lin

Liao

et al. 2008

Cell Mol Immunol

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In recent years, studies on evaluation of the therapeutic and toxic activity of herbal medicinal products became available and popular. The advances in modern biotechnology have led to discovery of many new active constituents. However, it is a constant challenge to establish the pharmacological basis for efficacy and safety of herbal medicinal products. A better understanding of the effects and bioavailability of phytopharmaceuticals can help in discovering suitable and rational therapies. In this review, we present the bioavailability studies in immune system that has been conducted for some of the more important or widely used phytopharmaceuticals. Furthermore, various new drug targets worthy of using for drug development in immunomodulating herbal medicine area and their regulatory mechanisms are also discussed. Adverse effects, drug interactions, and contraindications are also discussed which show that caution should be exercised when combining phytopharmaceuticals with chemically derived pharmaceutical components. Cellular & Molecular Immunology. 2008;5(1):23-31.

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“…Interestingly, the cytokine modulation effects of salidroside (group D) were less prominent than those of RRSS (group B), although the salidroside content in both groups was nearly equal. Our previous study showed that the effects of the individual components alone cannot substitute for or represent the cumulative effects of herbal medicine (Lin et al , ). However, this finding is quite different from our recent study, in which serum concentrations of IL‐2, IL‐4 and IL‐10 but not IFN‐γ were significantly increased after treatment with 1 mg/kg body weight cinnamaldehyde, but not significantly affected by treatment with Cinnamomum osmophloeum leaf essential oil groups that contained similar cinnamaldehyde levels (Chou Lin et al , ).…”

Section: Discussionmentioning

confidence: 99%

“…Cytokines (IL‐2, IL‐4, IL‐10 and IFN‐γ) were assayed in triplicate using commercial ELISA kits (Pharmingen, San Diego, CA, USA) according to the manufacturer's instructions, as described elsewhere (Tsai et al , ; Lin et al , ; Ho et al , ; Huang et al , ; Lin et al , , ; Lin et al , ). Using 96‐well plates, 100 μL anti‐mouse capture antibody diluted 1:250 was added and incubated overnight at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

In Vivo Th1 and Th2 Cytokine Modulation Effects of Rhodiola rosea Standardised Solution and its Major Constituent, Salidroside

Lin

Chin

Chao

et al. 2011

Phytotherapy Research

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Although Rhodiola rosea (L.) is used widely and disseminated in Oriental medicine, its in vivo effects on cytokine modulation remain unclear. Among the biologically active components of Rhodiola rosea, salidroside was suggested to be the most active compound. The objectives of this study were to assess the toxicity and cytokine modulation effects of Rhodiola rosea standardised solution (RRSS) and salidroside. Quantitative high pressure liquid chromatography (HPLC) analysis determined the content of salidroside in RRSS to be 4.39% (w/v). Groups of Balb/c mice were fed daily with different doses of RRSS or salidroside, with CAPE or distilled water used as positive and negative controls, respectively. The acute and subacute toxicity tests did not reveal weight differences, pathological changes, or abnormalities in liver or kidney function indices among the treated groups. Ovalbumin-primed mouse cytokine assays demonstrated that both T helper (Th1) (IL-2 and IFN-γ) and Th2 (IL-4 and IL-10) cytokines were significantly increased by feeding with RRSS in a dose- and time-dependent manner (p < 0.05). Moreover, the cytokine modulation effects of salidroside were less prominent than that of RRSS treatment and not dose-dependent. These findings suggest that increased secretion of both Th1- and Th2-pattern cytokines can be achieved with RRSS and salidroside treatment.

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In vitro anti-microbial and in vivo cytokine modulating effects of different prepared Chinese herbal medicines

Cited by 23 publications

References 31 publications

Assessing the Effect of Pineapple Extract Alone and in Combination With Vancomycin on Streptococcus sanguis

Assessing the Effect of Pineapple Extract Alone and in Combination With Vancomycin on Streptococcus sanguis

The Immunopharmaceutical Effects and Mechanisms of Herb Medicine

In Vivo Th1 and Th2 Cytokine Modulation Effects of Rhodiola rosea Standardised Solution and its Major Constituent, Salidroside

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