“…Those fractions were purified by preparative TLC on silica gel or by preparative HPLC to give isolicoflavonol (7) [22], isoglycycoumarin (8) [23], licoarylcoumarin (9) [24], formononetin (10) [25], and 6"-O-acetylliquiritin (11) [26]. On the other hand, the remaining part of the ethyl acetate fraction was directly subjected to column chromatography on ODS-gel, and fractions from the column were further purified by column chromatography on MCI-gel CHP-20P, and by preparative HPLC or preparative TLC, to give liquiritin (12) [27], p-hydroxybenzoic acid (13), semilicoisoflavone B (14) [28], glycyrol (15) [29], glycyrin (16) [29], gancaonin I (17) [30], isoglycyrol (18) [31], liquiritigenin (19) [27], gancaonin G (20) [36], glicoricone (25) [37], glycycoumarin (26) [38], licocoumarone (27) [29], licoricone (28) [39], glyasperin D (29) [40], isoangustone A (30) [41], and two additional compounds temporarily named compounds A (31), and B (32). Since several phenolics from licorice display potent antibacterial effects against methicillin-resistant Staphylococcus aureus (MRSA), and also show suppressing effects on the oxacillin resistance of MRSA, as we have reported previously [11], we have also investigated the effect of these licorice phenolics on VRE.…”