In-vitro anti-Vibrio spp. activity and chemical composition of some Tunisian aromatic plants

Abstract

The chemical composition of five aromatic plants (Mentha longifolia, M. pulegium, Eugenia caryophyllata, Thymus vulgaris and Rosmarinus officinalis) frequently used in food preparation in Tunisia was analysed by GC-MS. The antimicrobial effect of the essential oils obtained from these plants was tested against Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio fluvialis strains. Thyme oil exhibited a high level of antimicrobial activities against Vibrio spp. strains. The diameter of the zones of growth inhibition for V. parahaemolyticus species was interestingly high (ranging from 14.66 to 28 mm). The MIC and MBC values were interestingly low for thyme oil (MIC 0.078-0.156 mg/ml) and (MBC [0.31-1.25 mg/ml). These results showed that these plants especially thyme and clove, can be to be used for seafood preparation to protect against contamination by Vibrio spp. strains.

Effects of cold-storage facility characteristics on the virulence and sporulation of Penicillium expansum and the efficacy of essential oils against blue mold rot of apples

“…S. aromaticum essential oils from Tunisia show a high eugenol content ranging from 75.4% (Aguilar-Gonzalez et al 2015) to 85.3% (Pinto et al 2009). Studies in Morroco and Tunisia have found that the essence of M. pulegium is dominated by pulegone, with concentrations ranging from 44.27% to 85.4% (Snoussi et al, 2008;Hajlaoui et al, 2009;Hmiri et al, 2011).…”

Antagonistic activity of Thymus vulgaris extracts against Vibrio species isolated from seafoods

“…The sensitivity of microorganisms to thyme oil has been reported by Girova et al (2010). More so, the susceptibility of thymol, which is the most important constituent of thyme oil, has been examined by Snoussi et al (2008), Rivas et al (2010), Schirmer and Langsrud (2010) and Szczepaniak et al (2011).…”

“…Some studies have reported antibacterial activity of T. vulgaris (Ismail et al 2012). Snoussi et al (2008) also reported that plants like thyme and clove can be used for seafood preparation to protect against contamination by Vibrio species strains. Therefore there is a need to discover antibacterial compounds with innovative mechanisms of action for emerging antibiotic resistance Vibrio species.…”

Aceite esencial de Thymus vulgaris L (tomillo), su combinación con EDTA contra Cándida albicans y formulación de una crema

“…Es una planta introducida de Europa y en la medicina tradicional peruana se conoce como 'tomillo' y se utiliza el aceite esencial como antiséptico (6) . Se ha demostrado que el extracto de esta planta tiene efecto antiviral contra HSV-1, HSV-2 y HSV-1 resistente a aciclovir (7) y efecto relajante sobre anillos traqueales de cobayo comparable a teofilina (8) ; mientras que el aceite esencial de Thymus vulgaris ha mos-trado actividad antibateriana contra Staphylococcus aureus, Escherichia coli, Bacillus cereus, Proteus vulgaris, Vibrio spp, Listeria monocytogenes, Propionibacterium acnes (9,10,11) ; además, actividad citotóxica contra 3 líneas celulares de cáncer humano -células de carcinoma de próstata humano (PC-3), carcinoma pulmonar humano (A549) y cáncer de mama humano (MCF-7) (11) -, y significativa actividad antoxidante (12) .…”