In vitro Antibacterial Activity and In vivo Acute Toxicological Studies of Nelsonia campestris Aqueous Leaf Exrtact

Muhammad, Hadiza Lami; Makun, Hussaini Anthony; Kabiru, Adamu Yusuf; Mann, A.; Busari, Musa Bola; Abdullah, Abubakar Siddique; Aziz, Fátima

doi:10.9734/ijbcrr/2015/16081

Cited by 7 publications

(9 citation statements)

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“…Qualitative phytochemical analysis clearly demonstrated the presence of a number of important active constituents and revealed that both n-hexane and ethyl-acetate fractions of N. campestris have similar phytochemical constitution of alkaloids, phenols, tannis, flavonoids, saponins and steroids. The presence of these phytochemicals has been previously reported for crude aqueous extract of Nelsonia campestris [13]. Only the glycoside was present in n-hexane fraction but absent in ethyl-acetate fraction.…”

Section: Discussionmentioning

confidence: 53%

“…The collected fresh samples of Nelsonia campestris were washed with clean-water, dried at room temperature and finally grounded using a grinder mill and dried at room temperature. Extraction of plant materials was performed by weighing 50 g of the powdered plants and extracted by soxhlet extraction using 200 ml each of methanol [13]. The resulting extract was concentrated in a water bath and then fractionated with n-hexane and ethyl-acetate to give nhexane and ethyl-acetate fractions respectively.…”

Section: Sample Preparation and Extractionmentioning

confidence: 99%

“…The traditional healers use the root, fruit, and leaves of the plant for different disease conditions [12]. This plant, known as "Isa bakaggi," by the traditional practitioners of Nupe speaking people of Niger state, Nigeria, is used to treat measles caused by morbillivirus of the Paramyxovirus family, with concurrent development of other opportunistic infections such as pneumonia, conjunctivitis, diarrhea, and infection of the otitis media [13]. It is also used traditionally for the treatment of skin infections such as chicken pox, fevers, constipation and gastric ulcer, in addition to measles and lots of others.…”

Section: Introductionmentioning

confidence: 99%

“…It is also used traditionally for the treatment of skin infections such as chicken pox, fevers, constipation and gastric ulcer, in addition to measles and lots of others. Studies has documented the antibacterial and safety profile of the crude extract of this plant [12][13][14]. However, scientific literature on antimicrobial properties of solvent fractions of this plant is currently unavailable, the present study therefore evaluated the secondary metabolites compositions and in vitro antimicrobial profile of n-hexane and ethyl-acetate fractions of Nelsonia campestris leaf.…”

Section: Introductionmentioning

confidence: 99%

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Comparative secondary metabolite compositions and anti-microbial properties of n-hexane and ethyl-acetate fractions of Nelsonia campestris

Hadiza¹,

Lami

Hussaini³

2019

GSC Biol. and Pharm. Sci.

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Nelsonia campestris is a medicinal plant commonly used to treat measles caused by morbillivirus. This study assessed the phytochemical composition, in vitro antimicrobial activity of n-hexane and ethyl-acetate fractions of Nelsonia campestris. Phytochemical composition was determined using standard procedures. Antimicrobial test against six microorganisms, including K. pneumonia, S. aureus, B. subtilis, E. coli, P. aeruginosa and S. typhi was investigated using agar diffusion method while minimum inhibitory concentrations were determined by broth micro dilution method. Results revealed the presence of alkaloids, phenols, tannis, flavonoids, saponins and steroids in both n-hexane and ethyl-acetate fraction. Glycoside was present in n-hexane fraction but absent in ethyl-acetate fraction while volatile oil and anthraquinone were completely absent. Quantitatively, n-hexane fraction had higher phenol (57.12±0.01 mg/100g), tannins (19.76±0.04 mg/100g), and saponins (104.78±0.05 mg/100g) while ethyl-acetate had higher flavonoid (27.85±0.50 mg/100g) and alkaloids (16.62±0.01 mg/100g). The ethyl-acetate fraction was highly active against all test organisms with inhibition diameters in the range of 7.50±0.56 mm (P. aeruginosa) to 21.50±0.32 mm (E. coli) The MIC ranges between 2.5-20 mg/mL while the MBC ranged between 5.0-30 mg/ml for the ethyl-acetate fraction. The n-hexane fraction was active only against P. aeruginosa and B. subtilis with inhibition diameters in the range of 12.50±0.10 mm-17.55±1.00 mm. This study has shown that the n-hexane and ethyl-acetate fractions of Nelsonia campestris contain some useful potential bioactive principles that are inhibitory to some pathogenic organisms. The ethyl-acetate fraction was more active than n-hexane fraction and thus could be considered as a natural source of antimicrobials for therapeutic purposes.

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Section: Discussionmentioning

confidence: 53%

Section: Sample Preparation and Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative secondary metabolite compositions and anti-microbial properties of n-hexane and ethyl-acetate fractions of Nelsonia campestris

Hadiza¹,

Lami

Hussaini³

2019

GSC Biol. and Pharm. Sci.

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“…The experiment was performed under OECD guidelines 423. The methanolic seed extract of T. grandis was studied for acute toxicity study as reported by Muhammad et al with a little modification of two phases [21] . In first phase, a single dose of 10, 50, 100, 500 and 1000mg/kg (dissolved in water) body weight in test group, while mice in control group received normal saline was administered.…”

Section: Acute Toxicity Studiesmentioning

confidence: 99%

Acute oral toxicity study of Tectona grandis LInn. methanolic seed extract in albino mice

Dokuparthi¹,

Khan²,

Anusha³

et al. 2017

J Phytopharmacol

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Objective: To evaluate the acute toxicity studies of methanolic seed extract of Tectona grandis in albino mice. Method: The present study has been carried out to evaluate the adverse and hazardous effects of methanolic seed extract of Tectona grandis in albino mice through oral route. Acute oral toxicity of methanolic extract was carried out in two phases. In first phase, a single dose of 10, 50, 100, 500 and 1000mg/kg (dissolved in water) body weight in test group, while mice in control group received normal saline was administered. All the animals were individually studies for the mortality, wellness parameters and body weight for 14 days. The experiment was performed under OECD guidelines 423. No mortality or any significant physiological changes in the animals were observed up to 1000mg/kg bodyweight. The experiment was again repeated for second phase with a single doses of 1000, 1250, 1500 and 2000 mg/kg. Mortality was found at 1250 mg/kg. Therefore LD50 of the extract was estimated to be more than 1000mg/kg body weight. Finally, administration of a single dose of 10, 50, 100, 500, 1000 mg/kg of the extract did not showed any significant differences in food and water consumption and other behavioral changes. The extract starts showing toxicity at a dose of 1250 mg/kg body weight. Conclusion: The analysis of these results with the information of signs, behavior and health monitoring leads to the conclusion that the oral administration of T.grandis methanolic seed extract for 15 days does not cause acute toxicity. But, higher doses on long term administering can cause considerable toxicity.

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Acute and sub-acute toxicity studies of aqueous and methanol extracts of Nelsonia campestris in rats

Olaniyan

Muhammad

Makun

et al. 2016

Journal of Acute Disease

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In vitro Antibacterial Activity and In vivo Acute Toxicological Studies of Nelsonia campestris Aqueous Leaf Exrtact

Cited by 7 publications

References 0 publications

Comparative secondary metabolite compositions and anti-microbial properties of n-hexane and ethyl-acetate fractions of Nelsonia campestris

Comparative secondary metabolite compositions and anti-microbial properties of n-hexane and ethyl-acetate fractions of Nelsonia campestris

Acute oral toxicity study of Tectona grandis LInn. methanolic seed extract in albino mice

Acute and sub-acute toxicity studies of aqueous and methanol extracts of Nelsonia campestris in rats

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