In Vitro Antibacterial Activity of an Aqueous Extracts of the Tephrosia Vogelii Hook.f Combined To Imipenem on E. coli Strains

Abstract

Original Research ArticleBackground: To investigate, In Vitro, the antibacterial properties of Tephrosia Vogelii and its effects when combined with imipenem and to assess the level of bacterial resistance to imipenem and colistin. Methods: Sensitivity to colistin and imipenem was evaluated by the determination of Minimum Inhibitory Concentrations (MIC) in accordance with the recommendations of the Antibiogram Committee of the French Society of Microbiology (CASFM). The sensitivity of Tephrosia Vogelii was evaluated by the diffusion method in the wells in agar medium; while, the combined effects of the latter with imipenem were evaluated by the determination of MIC. Results: The MIC results showed that 55% of the E. coli strains are resistant to imipenem and 40% resistant to colistin. Tephrosia Vogelii has no antibacterial activity on E. coli strains. In addition, its association with imipenem led to the potentiation of the antibacterial activity of imipenem with a synergistic effect. Conclusion: The results may justify the use of the plant-antibiotic conventional combination against bacterial resistances to conventional antibiotics.

Colistin Resistance Profiles, Molecular Investigation of <i>mcr</i>-1 and <i>mcr</i>-2 Plasmid Genes and Investigation of Carbapenemase Production in <i>Pseudomonas</i> and <i>Acinetobacter</i> Strains

“…In Gabon, resistance to colistin was highlighted in 2019 by the work of Yala et al on enterobacteria, specifically E. coli, whose prevalence of resistance to colistin was 40% [17]. Furthermore, in the Salmonella and Shigella genera, the overall resistance to colistin was showed to be 20% [18].…”