In vitro Antibacterial Activity of Organic Extracts from North-West Moroccan Medicinal Plant Myrtus communis (L.)

Phenolic extracts of Centaurium erythraea with novel antiradical, antibacterial and antileishmanial activities

“…For the determination the diameter of inhibition of extracts against the tested bacteria, we have used agar-well diffusion assay as described in our previous studies [10][11][12]. A basal layer was prepared by Muller-Hinton agar.…”

“…MICs were determined using the broth micro-dilution assay as described in our previous studies [11,12]. LB medium containing 0.15% (w/v) of agar was used as a stabilizer of the extract-water mixture and the resazurin was used as a bacterial growth indicator.…”

“…After incubation at 37 °C for 18 h, 10 μL of resazurin was added to each well to assess bacterial growth. After further incubation at 37 °C for 2 h, the MIC was determined as the lowest concentration that prevented a change in resazurin color [11,12]. Bacterial growth was detected by reduction in blue dye resazurin to pink resorufin.…”

“…MBC corresponds to the lowest concentration of the extract yielding negative subcultures after incubation at appropriate temperature for 24 h [11,12]. It was determined in broth dilution tests by sub-culturing 10 μL from negative wells on PCA medium [11,12].…”

“…The most important sources of antioxidant, antibacterial and antiparasitic molecules are the natural bioactive products of plants such as flavonoids and phenolic compounds that have known for their several biological activies such as an antibacterial effect [10][11][12], suppress the production of free radicals [11,[13][14][15][16][17], delay oxidative stress reactions and have an important role against one of the world major human diseases like cancer [18]. In the search for such natural products and modern drugs, medicinal plants play an important role [19][20][21][22][23][24].…”