In-vitro Antibacterial Activity of Acacia Nilotica against Lactobacillus casei

Khan, Abid Ali; Ismail, Muhammad Owais; Baig, Mirza Tasawer; Kashif, Sadia Suri; Jabeen, Aisha; Sheikh, Shumaila; Shahid, Uzma

doi:10.9734/jpri/2021/v33i27a31489

JPRI

2021

DOI: 10.9734/jpri/2021/v33i27a31489

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In-vitro Antibacterial Activity of Acacia Nilotica against Lactobacillus casei

Abid Ali Khan

Muhammad Owais Ismail

Mirza Tasawer Baig

et al.

Abstract: Background: The role of Ethno-pharmacology is important to discover the new biologically active compounds. The process usually starts with searching of useful plants from different records to the development of methods for the industrial production of drugs. World Health Organization (WHO) states that more than 80% of population of the world makes the use of plants for the treatment of diseases. The extensive use of plants for therapeutic purposes has the history of centuries. Herbal pharmaceuticals have treat… Show more

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2024

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Cited by 2 publications

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Biological control of nosemosis in Apis mellifera L. with Acacia nilotica extract

El-Sayed,

Fathy,

Labib

et al. 2024

Sci Rep

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Nosemosis is one of the most devastating diseases of Apis mellifera (Honey bees) caused by the single-celled spore-forming fungi Nosema apis, N. ceranae and N. neumanii, causing a severe loss on the colony vitality and productivity. Fumagillin, a MetAP2 inhibitor, was a certified treatment for controlling nosemosis, nevertheless, due to its deleterious effects on honey bees and humans, it is prohibited. So, searching for novel biological agents with affordable selectivity to target Nosema species infecting Apis mellifera, with nil toxicity to bees and humans is the main objective of this study. Nosema species were isolated from naturally infected honey bees. The methanolic extracts of Acacia nilotica, Elaeis guineensis, and Catharanthus roseus were tested to selectively control the growth of Nosema spp of honeybees. The spores of Nosema species were molecularly and morphologically identified. Among the tested plant extracts, the methanolic extracts (0.1%) of A. nilotica had the most activity towards Nosema spp causing about 37.8 and 32.5% reduction in the spores’ load at 5- and 9-days post-infection, respectively, compared to the untreated control. At 0.1%, the A. nilotica methanolic extract exhibited the highest inhibitory effect for Nosema spores, without any obvious bee mortality. Catharanthus roseus displayed a reduction of spores by 27.02%, with bee mortality rate of 27.02%. At 1% for 5 dpi, the A. nilotica extracts led to 18.18% bee mortality, while the C. roseus extracts resulted in 100% mortality, as revealed from the toxicity and quantification bioassays. So, the extracts of A. nilotica and C. roseus had a significant effect in controlling the N. apis and N. ceranae titer compared to the infected untreated control at both time points. The titer of N. apis and N. ceranae was noticeably decreased by more than 80% and 90%, in response to A. nilotica, compared to the control. From the metabolic profiling by GC–MS analysis, the most frequent active compounds of A. nilotica were 2,4,6-trihy-droxybenzoic acid, 1,2-dihydroxybenzene, myristic acid, and linoleic acid. These compounds were analyzed in silico to assess their binding affinity to the ATP binding protein, methionine aminopeptidase and polar tube protein of Nosema species as target enzymes. The compound 2,4,6-trihydroxybenzoic acid had the lowest energy to bind with ATP binding protein, methionine aminopeptidase and polar tube protein of Nosema, followed by 1,2-dihydroxybenzene and myristic acid, compared to fumagilin. So, from the experimental and molecular docking analysis, the extracts of A. nilotica had the highest activity to attack the cellular growth machinery of Nosema species without an obvious effect to the honeybees, ensuring their prospective promising application.

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Biological control of nosemosis in Apis mellifera L. with Acacia nilotica extract

El-Sayed,

Fathy,

Labib

et al. 2024

Sci Rep

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Antimicrobial, Antioxidant Activities, and Phytochemical Characterization of Acacia nilotica and Hibiscus sabdariffa

Sara BA,

Esraa MA,

Azza HA

et al. 2024

Int J Agric Sc Food Technol

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Antibiotic abuse has caused the emergence of an alarming number of resistant pathogens. Hence, there is an urgent need for novel agents. Most of the Sudanese people in rural areas rely on traditional medicine for the treatment of many infectious diseases. This study was carried out to investigate the antimicrobial antioxidant activities and phytochemical contents of Hibiscus sabdariffa fruits and Acacia nilotica pods. Antimicrobial assays of the ethanolic extracts of both plants showed that they exhibit diverse inhibitory activity against Gram-positive Staphylococcus aureus, and three Gram-negative bacteria Salmonella typhi, Klebsiella and Escherichia coli, and one fungi Candida albicans, with the zone of inhibition ranging from 16 to 20 mm in length. The antioxidant activity of A. nilotica was about 91 %; it was highly significant compared with the standard (Propyl gallate) which gave (94%). While the extract of H. sabdariffa showed weak antioxidant activity. Phytochemical screening of A. nilotica indicates the presence of phenols, tannins, flavonoids, triterpenes, and saponins, while alkaloids were absent. The extract H. sabdariffa was rich in phenols, flavonoids, and alkaloids, but no tannins, triterpenes, or saponins were detected. These findings provide scientific evidence to support the traditional medicinal uses of these extracts and indicate the promising potential of these plants for medicinal purposes.

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In-vitro Antibacterial Activity of Acacia Nilotica against Lactobacillus casei

Cited by 2 publications

References 10 publications

Biological control of nosemosis in Apis mellifera L. with Acacia nilotica extract

Biological control of nosemosis in Apis mellifera L. with Acacia nilotica extract

Antimicrobial, Antioxidant Activities, and Phytochemical Characterization of Acacia nilotica and Hibiscus sabdariffa

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