In vitro Antibacterial Activity of Ocimum suave Essential Oils against Uropathogens Isolated from Patients in Selected Hospitals in Bushenyi District, Uganda

Tibyangye, Julius; Okech, Matilda A.; Nyabayo, Josephat Maniga; Nakavuma, Jessica L.

doi:10.9734/bmrj/2015/17526

Cited by 19 publications

(22 citation statements)

References 30 publications

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“…Overall prevalence of UTI in this study was found to be 13.5% with higher prevalence among females (23.1%) than in the males (6.2%). The prevalence of UTIs in the current study is similar to the finding by Pradhan et al 13 but is lower than the studies by Mwaka et al (10%), 14 Tibyangye et al (22%) 15 and Odoki et al (32%) 16 from Uganda; and Khatiwada et al from Nepal. 17 The possible explanation for the variation in the prevalence in these studies could be the differences in the types of the populations studied.…”

Section: Discussionsupporting

confidence: 87%

Antibiotic sensitivity pattern in urinary tract infection among adults in central part of Nepal

Sedhain

Sapkota²,

Shrestha³

2019

J Chitwan Med Coll

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Background: Urinary tract infection (UTI) is characterized by pathological invasion of the urinary tract by microorganisms. Majorities of organisms causing UTI are gram negative bacteria, most common of which is E. coli. Urine culture and sensitivity test is used to isolate the organism and to identify the susceptible drug of choice for appropriate treatment. This hospital-based study was carried out to analyze the spectrum and antibiotic susceptibility of microorganisms causing UTI. Methods: A retrospective study was done to analyze the results of urine culture and sensitivity test done at Chitwan Medical College Teaching Hospital over a period of two and half years. Standard guideline and protocol were used to collect the urine sample and to perform the test. Data analysis was done using SPSS version 21.0. Result: Out of 12, 925 urine samples submitted for culture and sensitivity test during the study period, bacterial pathogens were isolated from 3, 173 (24.54%) samples, which was significantly higher among females (28%) than the males (17%). The most common organism isolated on the culture was E. coli (68.5%) followed by Klebsiella (18.4%). Sensitivity of the uropathogens was seen highest with colistin (79.2%) followed by teicoplanin (64.58%), Aztreonam (63.25%) and Nitrofurantoin (61.16%). Most common antibiotics that showed resistance to the microorganisms in this study were Ampicillin (60.93%), Cotrimoxazole (53.72%), Cefixime (40.57%) and Levofloxacin (32.93%). Conclusions: This study has found a usual pattern of UTI with higher prevalence among females and E. coli being the most common organism. Nitrofurantoin has been found to have a good susceptibility for the treatment of UTI.

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Section: Discussionsupporting

confidence: 87%

Antibiotic sensitivity pattern in urinary tract infection among adults in central part of Nepal

Sedhain

Sapkota²,

Shrestha³

2019

J Chitwan Med Coll

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“…Lastly, the high prevalence of the MDR strains in E. coli and S. aureus in this study is in conformity with reports by Regmi et al (2018) and Mahato et al (2018) respectively. The high prevalence of MDR strains in E. coli in this study is of high concern, since E. coli is the most common cause of bacterial UTIs (Tibyangye et al, 2015;Odoki et al, 2019).…”

Section: Discussionmentioning

confidence: 80%

“…Where d=margin of error of setting a significance level of 0.05 (5%); z=level of significance (1.96) for confidence interval of 95%; p = prevalence of UTIs in Bushenyi District of Uganda, among patients receiving medical treatment at selected hospitals was 22.33% (Tibyangye et al, 2015). Patients of the following category were included in the study: symptomatic UTIs, suspected to have UTIs, and residents of Bushenyi District that are receiving medical treatment at KIU-TH, Comboni Hospital kyamuhunga and Ishaka Adventist Hospital.…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic analysis of multidrug resistant E. coli isolates from the urinary tract in Bushenyi district, Uganda using the new Clermont phylotyping method

Odoki¹,

Aliero²,

Tibyangye³

et al. 2020

Afr. J. Microbiol. Res.

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Due to the increasing rates of multidrug resistance (MDR) among the Enterobacteriaceae that cause urinary tract infections (UTIs), selection of antimicrobial agents for empirical therapy is becoming a major challenge. This study determined the antimicrobial resistance profiles, multidrug resistance profiles, multiple antibiotic resistance indices (MARI), factors associated with MDR UTIs and the phylogenetic groups of MDR Escherichia coli strains isolated from the urinary tract among patients attending hospitals in Bushenyi District, Uganda. In this crosssectional study, a total of 86 bacterial uropathogens isolated from 267 study participants suspected to have UTIs were subjected to antimicrobial susceptibility tests using the Kirby Bauer Disk diffusion method. Data for the factors associated with MDR were obtained by the use of questionnaires. Phylogenetic groups of the MDR E. coli were determined using the new Clermont method for phylotyping E. coli. Descriptive and multiple logistic regression statistical tools were used to determine phylogenetic groups, and assess for statistically significant relationship between MDR UTIs and factors suspected to be associated with MDR UTIs respectively. The isolates assigned as group B2 9/12 (75.0%), B1 2/5 (40.0%) and A 2/7 (28.6%) by using the old Clermont method could not be phylotyped using the new Clermont method and were grouped as non-typeable strains of E. coli. Our study demonstrated high prevalence of the non-typeable strains of MDR E. coli, we therefore recommend the use of modern DNA sequencing-based approaches which is the gold standard for genotyping bacteria, that this current study could not afford.

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“…The MIC was determined by the liquid microdilution method as previously described by several authors [17,21]. Briefly, two-fold geometric dilutions were carried out (from 1/2 to 1/256) and stock solutions of EHs were made in a preparation of Tween-20…”

Section: Minimum Inhibitory Concentration (Mic) Value Determination Amentioning

confidence: 99%

Assessment of the antibacterial activity of four essential oils and the biobactericide Neco.

Yala

Mabika

Camara

et al. 2017

ijpm

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The purpose of this study was to assess in vitro the activity of four essential oils (Cymbopogon citratus, Eucalyptus citriodora, Lippia multiflora, Melaleuca quinquenervia) and the biobactericide Neco® on Gram-positive bacteria.The aromatogram and antibiogram were assessed by the agar well diffusion method and the Muller Hinton disk-agar diffusion method, respectively. Also, the minimum inhibitory concentration and the minimum bactericidal concentration were determined by the microdilution method in liquid medium.The aromatogram showed that the biobactericide Neco® induced the largest inhibition diameters (34.53 ± 11.82 - 43.92 ± 5.38 mm) of all strains combined, followed by the essential oils of Eucalyptus Citriodora (30.01 ± 3.02 - 41.89 ± 1.77 mm) and Lippia multiflora (20.72 ± 4.72 - 37.61 ± 2.80 mm). However, the essential oils of Melaleuca quinquenervia (19.99 ± 3.93 - 26.20 ± 13.27 mm) and Cymbopogon citratus (13.52 ± 3.59 - 29.08 ± 2.35 mm) had the smallest inhibition diameters. Moreover, the comparison of the activities of the aromatogram and antibiogram revealed generally that activities were higher with essential oils than with antibiotics.At the end of this study, the essential oils of Cymbopogon citratus, Eucalyptus citriodora, Lippia multiflora, Melaleuca quinquenervia and the biobactericide Neco® had an antibacterial activity on Gram+ bacteria.

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In vitro Antibacterial Activity of Ocimum suave Essential Oils against Uropathogens Isolated from Patients in Selected Hospitals in Bushenyi District, Uganda

Cited by 19 publications

References 30 publications

Antibiotic sensitivity pattern in urinary tract infection among adults in central part of Nepal

Antibiotic sensitivity pattern in urinary tract infection among adults in central part of Nepal

Phylogenetic analysis of multidrug resistant E. coli isolates from the urinary tract in Bushenyi district, Uganda using the new Clermont phylotyping method

Assessment of the antibacterial activity of four essential oils and the biobactericide Neco.

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