In Vitro Antibacterial Activity of Biological-Derived Silver Nanoparticles: Preliminary Data

Abstract

Silver nanoparticles (AgNPs) are promising alternatives to antibiotics. The aims of this study were to produce AgNPs using two biological methods and determine their antibacterial activity against Pseudomonas aeruginosa and Staphylococcus pseudintermedius. AgNPs were biosynthesized from an infusion of Curcuma longa (turmeric) and the culture supernatant of E. coli. Characterization was achieved by ultraviolet-visible spectroscopy and by Transmission Electron Microscopy (TEM). The antibacterial properties of NPs from C. longa (ClAgNPs) and E. coli (EcAgNPs), alone and in combination with carbenicillin and ampicillin, were investigated through the Kirby-Bauer disk diffusion assay and the minimum inhibitory concentration (MIC). Dimensions of NPs ranged from 11.107 ± 2.705 nm (ClAgNPs) to 27.282 ± 2.68 nm (EcAgNPs). Kirby-Bauer and MIC assays showed great antibacterial abilities for both NPs alone and in combination with antibiotics. EcAgNPs alone showed the most powerful antibacterial activities, resulting in MIC values ranging from 0.438 ± 0.18 µM (P. aeruginosa) to 3.75 ± 3.65 µM (S. pseudintermedius) compared to those of ClAgNPs: 71.8 ± 0 µM (P. aeruginosa) and 143.7 ± 0 µM (S. pseudintermedius). The antibiofilm abilities were strain-dependent, but no statistical differences were found between the two NPs. These results suggest the antibacterial potential of AgNPs for the treatment of infectious diseases.

Antibiofilm activity of silver nanoparticles against biofilm forming Staphylococcus pseudintermedius isolated from dogs with otitis externa

“…The abilities against S. pseudintermedius were not identified. In this study, we verified the antibiofilm abilities of AgNPs at various concentration compared with a study reported previously (Meroni et al., 2020).…”

“…Some products intended for dogs that contain AgNPs are also available, but little is known about their microbial activity against veterinary relevant pathogens (Bae et al., 2017). A single preliminary study of the antibiofilm activity of AgNPs on S. pseudintermedius isolated from dogs currently exists (Meroni et al., 2020).…”

A Biological Study of Anisotropic Silver Nanoparticles and Their Antimicrobial Application for Topical Use

“…The results show that the antimicrobial efficiency was followed by S1, S2, S3, S4 and S5, which can be divided into two groups: S1–S2 (MIC/MBC ≤ 32 µg/mL) and S3–S5 (MIC/MBC ≤ 100 µg/mL). Our results are the same as a previous report by Gabriele Meroni et al, 2020 and Katarzyna A et al, 2017 [ 39 , 40 ]; AgNPs have strong antibacterial abilities to S. pseudintermedius . In a comparison of all AgNPs, S1 and S2 were very close in MIC and MBC.…”

Biogenic Silver Nanoparticles Strategically Combined With Origanum vulgare Derivatives: Antibacterial Mechanism of Action and Effect on Multidrug-Resistant Strains

“…Combinations containing conventional antibacterials are already practiced in clinical settings to combat resistant Gram-positive and Gram-negative strains (Eades et al, 2017;Jacobs et al, 2017;Doi, 2019). BioAgNP showed antimicrobial synergistic or additive effect when combined with several essential oils, their main constituents, and other natural compounds (Cardozo et al, 2013;Biasi-Garbin et al, 2015;Otaguiri et al, 2016;Scandorieiro et al, 2016;Dehkordi et al, 2019), and some conventional antimicrobials and other materials or drugs (Kora and Rastogi, 2013;Longhi et al, 2016;Andrade et al, 2017;Bankier et al, 2019;Bocate et al, 2019;Figueiredo et al, 2019;Vazquez-Muñoz et al, 2019;Meroni et al, 2020) such as phenazine-1-carboxamide, eugenol, oregano oil, copaiba oil, carbon dots, tungsten carbide nanoparticles, cupper nanoparticles, simvastatin, fluconazole, carbenicillin, streptomycin, ampicillin, tetracycline, kanamycin, and chloramphenicol. Other studies have also reported an antimicrobial synergistic or additive effect of oregano derivatives in combination with others natural compounds (Ayari et al, 2020;Cho et al, 2020) and several conventional antimicrobials (Hamoud et al, 2014;Langeveld et al, 2014;Yap et al, 2014;Magi et al, 2015;Xiao et al, 2020) such as cinnamon essential oil, thyme-derived Thy, cinnamon bark essential oil, erythromycin, fluoroquinolones, doxycycline, lincomycin, vancomycin, amoxicillin, gentamicin, levofloxacin, ciprofloxacin, rifampin, and polymyxin.…”