In vitro antibacterial and antioxidant properties of Elettaria cardamomum Maton extract and its effects, incorporated with chitosan, on storage time of lamb meat

Abstract

________________________________________________________________________________________ SHARAFATI-CHALESHTORI, F., R. SHARAFATI-CHALESHTORI:In vitro antibacterial and antioxidant properties of Elettaria cardamomum Maton extract and its effects, incorporated with chitosan, on storage time of lamb meat. Vet. arhiv 87, 301-315, 2017. ABSTRACTIn this experimental study, ethanolic extract of Elettaria cardamomum Maton (ECM) was prepared, the amount of phenolic compounds and antioxidant properties were measured using colorimetric methods and β-carotene bleaching assay, respectively. The effects of chitosan with a concentration of 1% and 2% (w/w) of ECM extract at refrigerated temperature (4 ± 1 °C) on the microbial quality of lamb meat was evaluated. Changes in pH level, the total count of bacteria, psychrotrophic and lactic acid bacteria, Enterobacteriaceae, yeast, mold counts and also the organoleptic characteristics of lamb meat in specific storage times (day zero, 1 st , 3 rd and 5 th ) were examined. The total amount of phenols of ECM ethanolic extract was 19.27 ± 0.02 mg/g Gallic acid equivalent, and antioxidant capacity was 45.7 ± 7.5%. In all treated samples, a reduction in the total count of bacteria, Enterobacteriaceae, psychrotrophic and lactic acid bacteria, molds and yeasts were observed (P<0.05). Besides, the addition of extract incorporated with chitosan to meat increased its overall acceptance rate (P<0.05). It may be concluded that ethanolic extract of ECM mixed with chitosan can be used as a natural antibacterial and antioxidant coating, safe to be consumed with meat products.

Chemical Composition, Antioxidant and Antibacterial Activity of Bunium persicum, Eucalyptus globulus, and Rose Water on Multidrug-Resistant Listeria Species

“…Previous works have demonstrated the antioxidant activity and antibacterial properties of essential oils and extracts. 2 , 23 , 25 , 26 …”

“…Previous works have demonstrated the antioxidant activity and antibacterial properties of essential oils and extracts. 2,23,25,26 Abbreviations: E, ethanolic extract; EO, essential oil; NE, no effect. Our results showed that B persicum, E globulus, and rose water had antioxidant activities (Figure 1), which was supported by previous studies.…”

“…The obtained absorbance rate was posed at the standard curve and thus the total phenol content of the extract in mg/g gallic acid equivalent was estimated. 23 …”

Optimization of a chitosan solution as potential carrier for the incorporation of Santolina chamaecyparissus L. solid by-product in an edible vegetal coating on ‘Manchego’ cheese

“…The application of active compounds present in such residues may well be through their incorporation in diverse biodegradable and edible matrices as, among others, galactomannan, sodium alginate or particularly chitosan (Costa, Maciel, Teixeira, Vicente, & Cerqueira, 2018), which has demonstrated good preservative capacities on sheep's (Cerqueira et al, 2009) and goat's (Cano-Embuena et al, 2017) cheeses. Furthermore, chitosan has previously demonstrated capacities as carrier for the incorporation of different vegetal extracts (Bonilla & Sobral, 2016;Kalaycıoğlu, Torlak, Akın-Evingür, Özen, & Erim, 2017;Shahbazi, 2017;Sharafati-Chaleshtori & Sharafati-Chaleshtori, 2017;Siripatrawan & Harte, 2010) and incorporated as GRAS (Generally Recognized as Safe) by the Food and Drug Administration (FDA, 2002) and in the Union list of novel foods under the Regulation (EU) No 2017/2470 of 20 December 2017.…”

Chemical Composition, Antioxidant Potential, and Antimicrobial Activity of Elettaria cardamomum Essential Oil

“…They reported that the highest inhibitory activity was observed against S. typhi (26) . Sharafati et al (2017) reported that the green cardamom ethanolic extract had an antibacterial effect against Enterobacteriaceae (27) . Naeini et al (2011) examined the antifungal effects of 50 herbal medicines using the disk diffusion method.…”