In Vitro Antibacterial and Antiproliferative Potential of Echinops lanceolatus Mattf. (Asteraceae) and Identification of Potential Bioactive Compounds

Abstract

Many species belonging to the genus Echinops are widely used in traditional medicine to treat infectious diseases and cancers. The present study aimed to evaluate the antibacterial and antiproliferative properties of Echinops lanceolatus Mattf. (Asteraceae). The activity of the methanolic extract and subsequent partition fractions was investigated against drug-resistant bacteria (Gram-negative and Gram-positive) and human tumor cell lines using broth microdilution and sulforhodamine B (SRB) assay, respectively. Our findings revealed weak to moderate antibacterial activities of tested extracts, with the recorded minimal inhibitory concentrations ranging from 256 to 1024 µg/mL. The ethyl acetate fraction (EL-EA) was found to be the most effective. Likewise, that fraction displayed strong antiproliferative potential with recorded IC50 of 8.27 µg/mL and 28.27 µg/mL on A549 and HeLa cells, respectively. An analysis based on the ultra-performance liquid chromatography–electrospray ionization tandem mass spectrometry (UPLC–ESI–MS/MS) of the EL-EA fraction allowed the identification of 32 compounds, of which quinic acid and derivatives, cinnamic acid derivatives, dihydrokaempferol, naringenin-7-O-glucoside, apigenin-7-O-d-glucoside, naringin, apigenin, rhoifolin, coniferyl aldehyde, and secoisolariciresinol are well-known compounds of biological importance. This study is first to report on the biological activity and phytochemical profile of E. lanceolatus. We provide a baseline to consider E. lanceolatus as a valuable source of anti-infective and antiproliferative agents.

LC-MS/MS Screening of Phenolic Compounds in Wild and Cultivated Grapes Vitis amurensis Rupr.

“…The anthocyanins identified in V. amurensis in this study were previously identified and annotated in the vines [17] Solanium nigrum [18], Gaultheria Antarctica [19], and Vitis vinifera [20] and wheat [21]. Our identification of flavonoid compounds agrees with bibliographic data for Echinops [22], Rhodiola rosea [23], Ocimum [24], Alpinia officinarum [25], Brazilian propolis [26], Vitis vinifera [20], Rubus occidentalis [27], C. edulis [28], and Vaccinium macrocarpon [29].…”

“…In general, the diversity of phytochemicals identified in wild and cultural grape V. amurensis resulted in the following descending order (number of metabolites in parenthesis): VZK (52) > ART (46) > SPB-2 (39) > SPB-1 (28) > SPB-4 (27) > PAK (25) > RIK (22) > KAL (20) > SPB-3 (19) > ARS (18). The most diverse metabolome was identified in the grapes collected in the vicinity of Vyazemsky, Khabarovsk Territory, which was rich in flavanols and phenolic acids.…”

Rosa davurica Pall., Rosa rugosa Thumb., and Rosa acicularis Lindl. Originating from Far Eastern Russia: Screening of 146 Chemical Constituents in Three Species of the Genus Rosa

“…The flavonols Kaempferol (compound 20), Dihydrokaempferol (compound 21), Kaempferol-3-O-α-L-rhamnoside (compound 30), Kaempferol diacetyl hexoside (compound 34), Kaempferol 3-O-rutinoside (compound 35), and Kaempferol 3-O-deoxyhexosylhexoside (compound 36) have been already characterized as components of F. glaucescens [25], Andean blueberry [28], Rhus coriaria (Sumac) [32], Lonicera japonica [34], Potato leaves [35], Rapeseed petals [36], Echinops lanceolatus [37], Camellia kucha [38]. Thus, the flavonol Kaempferol was found in extracts of R. rugosa, R. davurica, and R. acicularis.…”

Understanding the Ethnobotany, Chemistry, Pharmacology, and Distribution of Genus Hydnora (Aristolochiaceae)

“…Additionally, activity results were given in minimum inhibitory concentration (MIC). In plants, antibacterial activity is low if the minimum inhibitory concentration is greater than 625 µg/mL, high when the MIC is less than 100 µg/mL, and moderate if the MIC is between 100 µg/mL and 625 µg/mL [ 76 ]. This criterion is normally applied in deducing both antibacterial and antifungal activities.…”