In Vitro Antibacterial and Antitumor Activity of Total Triterpenoids from a Medicinal Mushroom Sanghuangporus sanghuang (Agaricomycetes) in Liquid Fermentation Culture

Xiao, Jie; Cai, C.; Liu, Jing Ju; Gao, Su; Zhao, Guo Zhu; He, Xiang Wei

doi:10.1615/intjmedmushrooms.2021038916

Cited by 10 publications

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“…Sanghuang possesses antitumor [ 28 ], antioxidant [ 29 ], anti-inflammatory [ 30 ], hypoglycemic, hypolipidemic [ 31 ], and immunomodulatory activities [ 32 ]. The bioactive compounds of Sanghuang mainly are comprised of triterpenoids [ 33 ], polysaccharides [ 34 ], and flavonoids [ 35 ]. Hispidin is an important bioactive flavonoid of Sanghuang [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Putative Polyketide Synthase Gene Involved in Hispidin Biosynthesis in Sanghuangporus sanghuang

Wei,

Liu,

Yuan

et al. 2023

Mycobiology

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Hispidin is an important styrylpyrone produced by Sanghuangporus sanghuang . To analyze hispidin biosynthesis in S. sanghuang , the transcriptomes of hispidin-producing and non-producing S. sanghuang were determined by Illumina sequencing. Five PKSs were identified using genome annotation. Comparative analysis with the reference transcriptome showed that two PKSs ( ShPKS3 and ShPKS4 ) had low expression levels in four types of media. The gene expression pattern of only ShPKS1 was consistent with the yield variation of hispidin. The combined analyses of gene expression with qPCR and hispidin detection by liquid chromatography-mass spectrometry coupled with ion-trap and time-of-flight technologies (LCMS-IT-TOF) showed that ShPKS1 was involved in hispidin biosynthesis in S. sanghuang. ShPKS1 is a partially reducing PKS gene with extra AMP and ACP domains before the KS domain. The domain architecture of ShPKS1 was AMP-ACP-KS-AT-DH-KR-ACP-ACP. Phylogenetic analysis shows that ShPKS1 and other PKS genes from Hymenochaetaceae form a unique monophyletic clade closely related to the clade containing Agaricales hispidin synthase. Taken together, our data indicate that ShPKS1 is a novel PKS of S. sanghuang involved in hispidin biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals the Putative Polyketide Synthase Gene Involved in Hispidin Biosynthesis in Sanghuangporus sanghuang

Wei,

Liu,

Yuan

et al. 2023

Mycobiology

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“…It was later distinguished as a new species, Inonotus sanghuang , in 2012 [ 2 ] and retitled S. sanghuang in 2016 [ 3 ]. Numerous studies have shown that S. sanghuang exhibits various biological activities such as hypoglycemic [ 4 ], antioxidant [ 5 , 6 ], antitumor, antibacterial [ 7 ], antidiabetic [ 8 ], and anti-inflammatory effects [ 9 ]. Triterpenoids are the main active components of S. sanghuang [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and Metabolome Integration Reveals the Impact of Fungal Elicitors on Triterpene Accumulation in Sanghuangporus sanghuang

Zhou

Zhang

et al. 2023

JoF

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Sanghuangporus sanghuang is a large wood-decaying mushroom highly valued in traditional Chinese medicine due to its medicinal properties, including hypoglycemic, antioxidant, antitumor, and antibacterial properties effects. Its key bioactive compounds include flavonoids and triterpenoids. Specific fungal genes can be selectively induced by fungal elicitors. To investigate the effect of fungal polysaccharides derived from Perenniporia tenuis mycelia on the metabolites of S. sanghuang, we conducted metabolic and transcriptional profiling with and without elicitor treatment (ET and WET, respectively). Correlation analysis showed significant differences in triterpenoid biosynthesis between the ET and WET groups. In addition, the structural genes associated with triterpenoids and their metabolites in both groups were verified using quantitative real-time polymerase chain reaction (qRT-PCR) and high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). Through metabolite screening, three triterpenoids were identified: betulinol, betulinic acid, and 2-hydroxyoleanolic acid. Excitation treatment increased the level of betulinic acid by 2.62-fold and 2-hydroxyoleanolic acid by 114.67-fold compared to WET. The qRT-PCR results of the four genes expressed in secondary metabolic pathways, defense gene activation, and signal transduction showed significant variation between the ET and WET groups. Overall, our study suggests that the fungal elicitor induced the aggregation of pentacyclic triterpenoid secondary metabolites in S. sanghuang.

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“…Until 1968, modern pharmacological investigation on sanghuang was unlocked based on the finding of its remarkable anti-tumor activity by Ikekawa et al ( 1968 ). As a group of ancient, precious, natural, and medicinal fungal resources, sanghuang has been validated by extensive scholars to have fascinating therapeutic functionalities, such as anti-tumor (Huang et al, 2011 ; He et al, 2021a ), antioxidant (Liu et al, 1997 ; Shon et al, 2003 ; Chen et al, 2016 ), anti-bacterial (Lu et al, 2020 ; Ma et al, 2021 ), anti-virus (Mirzadeh et al, 2020 ), anti-angiogenic (Lee Y. S. et al, 2010 ), anti-platelet (Kamruzzaman et al, 2011 ), anti-inflammatory (Kim B. C. et al, 2006 ; Lin et al, 2017 ), immunoregulatory (Meng et al, 2016 ; Azeem et al, 2018 ; Jung and Kang, 2020 ), and hepatoprotective activities (Shan et al, 2019 ; Yang et al, 2020 ), treating diabetes (Hwang et al, 2005 ; Ajith and Janardhanan, 2021 ), hyperlipidemia (Rony et al, 2014 ), and cardiovascular (Lee et al, 2007 ; Su et al, 2017 ) diseases, etc.…”

Section: Introductionmentioning

confidence: 99%

Current advances and potential trends of the polysaccharides derived from medicinal mushrooms sanghuang

Wang

Zhou

et al. 2022

Front. Microbiol.

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For thousands of years, sanghuang is distinctive as a general designation for a group of precious and rare Chinese medicinal mushrooms. Numerous investigations have revealed that polysaccharide is one of the important biological active ingredients of sanghuang with various excellent biological activities, including antioxidant, anti-aging, anti-tumor, immunomodulatory, anti-inflammatory, anti-diabetic, hepatoprotective, and anti-microbial functionalities. For the past two decades, preparation, structural characterization, and reliable bioactivities of the polysaccharides from fruiting bodies, cultured mycelia, and fermentation broth of sanghuang have been arousing extensive interest, and particularly, different strains, sources, and isolation protocols might result in obvious discrepancies in structural features and bioactivities. Therefore, this review summarizes the recent reports on preparation strategies, structural features, bioactivities, and structure-activity relationships of sanghuang polysaccharides, which will enrich the knowledge on the values of natural sanghuang polysaccharides and support their further development and utilization as therapeutic agents, vaccines, and functional foods in tonic and clinical treatment.

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In Vitro Antibacterial and Antitumor Activity of Total Triterpenoids from a Medicinal Mushroom Sanghuangporus sanghuang (Agaricomycetes) in Liquid Fermentation Culture

Cited by 10 publications

References 0 publications

Transcriptome Analysis Reveals the Putative Polyketide Synthase Gene Involved in Hispidin Biosynthesis in Sanghuangporus sanghuang

Transcriptome Analysis Reveals the Putative Polyketide Synthase Gene Involved in Hispidin Biosynthesis in Sanghuangporus sanghuang

Transcriptome and Metabolome Integration Reveals the Impact of Fungal Elicitors on Triterpene Accumulation in Sanghuangporus sanghuang

Current advances and potential trends of the polysaccharides derived from medicinal mushrooms sanghuang

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