In vitro antibacterial and cytogenotoxicological properties of the aqueous extract of Cymbopogoncitratus Stapf (DC) leaf

Abstract

BackgroundMicrobial infection of the genital tract or semen is one of the leading causes of male infertility. Consequently, there is a need to seek alternative products from natural sources.ObjectivesThe antibacterial, phytochemical and cytogenotoxicological assessments of the aqueous extract of Cymbopogon citratus leaf were evaluated.MethodsThe antibacterial potential of the extract was done via agar-well diffusion and microdilution techniques. The phytochemical analysis was done via standard protocols.The cytogenotoxicity of the extract were analyzed using the Allium cepa assay.ResultsAll test organisms were found to be sensitive to the extract except Pseudomonas. aeruginosa where no measurable zone of inhibition could be ascertained at all concentrations assessed.The highest mean inhibition diameter of 21.33±1.20mm against S. sapophyticus was recorded and a concentration-dependent susceptibility noticed. The phytochemical results revealed the presence of saponins, flavonoid, glycoside, steroids, terpenoid and alkaloids. The Alliumcepa root showed reduced mitotic indices following aconcentration-dependent increase in the extract.It can be said that the aqueous extract of C. citratus had inhibitory activities against the tested pathogenic organisms with relative anti-tumour potential.ConclusionThis study indicated, C. citratus could be a potential source for antibacterial compounds for the possible treatment of male reproductive related infections.

Effect of Adding Different Levels of Aqueous Extract of Lemongrass Leaves on Reproductive Characteristics, Testis Weight and Testicular Tissues of Male Broiler Mothers ROSS308

“…It noted a decrease in the fourth treatment with a concentration of 400 ml/litre. This result agreed with the researchers [23]. The high concentration of lemongrass aqueous extract significantly affects the values of semen and leads to the formation of abnormal cells with increasing concentrations and may be toxic at high concentrations, and the effect on Sertoli cells that participate in improving the reproductive performance of roosters is not raising the properties of the fluid but it also stops the formation of free roots [24].…”

Potential of Antibacterial Activity in Natural Ingredients (Betel Leaves, Basil Leaves, Avocado Seeds, Lemongrass, Garlic) As Natural Hand Sanitizers: Journal Review

“…The phytochemical analysis identifies active compounds that have the potential as antibacterial substances in natural extracts [25]. A literature review of several journals (listed in Table 2) found that some of the phytochemical content contained in betel leaves, basil leaves, avocado seed, lemongrass, and garlic [1,4,5,12,17]. Table 2 proves that betel leaves, basil leaves, avocado seeds, lemongrass, and garlic contain phytochemical compounds in alkaloids, flavonoids, saponins, phenols, tannins, and terpenoids.…”

“…Plants classified as antibacterial compounds are terpenoid, flavonoid, saponin, alkaloid, tannin, and phenol [1]. Some of these compounds are present in betel leaves [2], basil leaves [3], avocado seeds [1], lemongrass [4], and garlic [5]. Research [6] showed that eugenol compounds in methanol extract of betel leaves could inhibit the growth of gram-positive bacteria: Bacillus sp; E.Faecalis; S. aureus; S. agalactiae and gram-negative bacteria: A. hyrophila; E.coli; K. pneumoniae; P. aeruginosa; V. alginolyticus with an inhibition zone of 0.5-2.0 cm.…”

Infections: HIV and related-diseases, anti-microbial resistance and neglected tropical diseases

“…Nigerian authors report that Dengue is a growing public health problem in their country12, and that levels of stigma against Buruli ulcer disease are high13. On the surgical front, Salako et al report that 10% of patients who underwent prostatectomy in a Nigerian Teaching Hospital suffered from a surgical site infection14, while Omorogiuwa et al report that Cymbopogoncitratus Stapf leaf is a potential treatment of male reproductive related infections15.…”