In Vitro Antibacterial Experiment of Fuzheng Jiedu Huayu Decoction Against Multidrug-Resistant Pseudomonas aeruginosa

Abstract

Objective: Drawing a growth curve of multidrug-resistant Pseudomonas aeruginosa (MDR-PA) provides a foundation for susceptibility testing. By observing in vitro antibacterial activity and ultrastructure cthanges on MDR-PA of the effective components in the drug-containing serum of rats after the administration of Fuzheng Jiedu Huayu decoction (FJHD), we evaluated the inhibition and direct destruction effect of bacteria by TCM alone or combined with antibiotics. Methods: The absorbance values of MDR-PA were determined at different detection time points, and a growth curve was drawn. After gavage with FJHD, drug-containing serum was collected from the rats. Using Imipenem/cilastatin sodium as the positive drug control, the in vitro antibacterial potency of FJHD and its drug-containing serum alone or in combination with antibiotics against MDR-PA was observed. The ultrastructural changes of MDR-PA treated by FJHD combined with antibiotics were observed by transmission electron microscopy. Results: Growth of the experimental strain manifested a lag phase in the first 1-4 h, an exponential growth phase at 5-20 h, and a plateau phase after 20 h. The best detection time during the susceptibility test was 16-20 h. The minimum inhibitory concentration (MIC) value of the FJHD extract group was 0.2 g/mL. The MIC value of the pure Imipenem/ cilastatin sodium group was 16 mg/mL. The MIC values of Imipenem/cilastatin sodium + blank serum, 0.5-, 1-, and 2-fold drug-containing serum groups were all 16 mg/mL. The MIC values of Imipenem/cilastatin sodium + 4-and 8-fold drug-containing serum groups were both 8 mg/mL. By observation under a transmission electron microscope, Imipenem/ cilastatin sodium + 0.5-, 1-, and 2-fold drug-containing serum groups showed bacterial structural damage. The degree of bacterial destruction was more obvious and the quantity

Copper/Zinc-Modified Palygorskite Protects Against Salmonella Typhimurium Infection and Modulates the Intestinal Microbiota in Chickens

“…The inhibition ability of bacteria can be estimated by a susceptibility assay in vitro ( Jiang et al, 2019 ; Gámez et al, 2020 ), and a bacterial growth curve forms the basis of the susceptibility test ( Xu H. et al, 2020 ). The current study showed that there was no inhibition zone around natural Pal, which is consistent with a previous study that natural clay minerals have no antibacterial activity ( Rapacz-Kmita et al, 2017 ).…”

In Vitro Antibacterial Experiments of Qixingjian Decoction and Its Synergistic Interaction with Oxacillin against Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

“…The growth curve of MRSA was performed according to Xu et al [ 15 ] with some modifications. The optimum detection wavelength of MRSA absorbance was selected at 630 nm.…”

Traditional Chinese Medicine is an Alternative Therapeutic Option for Treatment of Pseudomonas aeruginosa Infections

“…A variety of TCMs have shown bactericidal effects against respiratory pathogens by directly killing or inhibiting their growth (Ooi et al, 2006;Liu et al, 2007;Yamada et al, 2016), and some of them could enhance the in vitro antibacterial effects of antibiotics in treatment of MDR P. aeruginosa infections (Liu et al, 2007;Xu H. et al, 2019), possibly due to the enhanced stability of antibiotics by the reductive components of TCM (Gim et al, 2009;Chen et al, 2013;Matkowski et al, 2013;Sun W. et al, 2014;Chen et al, 2020), and the inhibitory effects of TCM on bacterial efflux pumps (Huang et al, 2015;Wang et al, 2018;Wang J. et al, 2019), suggesting that TCM could be a good alternative or complement for synthetic antibiotics. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of TCM against P. aeruginosa were summarized in Table 4.…”

“…ma-yuen (Rom.Caill.) Stapf (Poaceae) with a mixture ratio of 2.5: 2.5:2.5:2.5:5:1:5:5 (Xu H. et al, 2019). Furthermore, the Fuzheng Jiedu Huayu formula decoction has been applied to treat elderly patients with pneumonia in clinical trials, and no obvious adverse reactions were manifested (Xu et al, 2018).…”

“…Furthermore, the Fuzheng Jiedu Huayu formula decoction has been applied to treat elderly patients with pneumonia in clinical trials, and no obvious adverse reactions were manifested (Xu et al, 2018). Xu et al revealed that the Fuzheng Jiedu Huayu decoction with an MIC of 200 mg/ml against P. aeruginosa combined with Imipenem/cilastatin sodium could enhance the in vitro bactericidal and bacteriostatic effects of Imipenem/cilastatin sodium on MDR P. aeruginosa (Xu H. et al, 2019).…”