IN-VITRO ANTIBACTERIAL, PHYTOCHEMICAL, ANTIMYCOBACTERIAL ACTIVITIES AND GC-MS ANALYSES OF Bidens pilosa LEAF EXTRACT

Abstract

The phytochemical constituents, antimicrobial activity, anti-mycobacterial activity and gas chromatography-mass spectrometry (GC-MS) analysis of the West African ecotype of Bidens pilosa was investigated for possible medicinal properties. The antimicrobial activity of the hexane, dichloromethane, ethyl acetate and methanol extracts from the leaf of Bidens pilosa was evaluated using agar dilution method. The qualitative and quantitative phytochemical screening was carried out according to standard procedures. Partitioned fractions of the methanolic extract was subjected to anti-mycobacterial bioassay. Different fractions of the leaf were subjected to GC-MS to ascertain the compounds present. The antimicrobial analysis revealed the methanolic fraction as having the highest number of activity against test organisms such as: Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans and Rhizopus sp. between 10 – 40 mm. The minimum inhibitory concentration showed the methanolic fraction to be active against Candida albicans and Rhizopus sp. at the concentration of 6.25 g/ml and 3.25 g/ml respectively. The phytochemical screening revealed the presence of alkaloids, cardiac glycosides and terpenoids in all the solvents. Tannin was present in all the solvent fractions except hexane fraction. Saponin was not found in any of the solvents. The hexane-methanol interface of the partitioned solvents was sensitive to the anti-mycobacterial activity while other solvents showed resistance. The GC-MS and the chromatogram gave insight into the volatile components of the leaf extract. The findings reveals Bidens pilosa as a medicinal plant with potentials for the treatment of tuberculosis.

Analysis of bioactive compounds present in different crude extracts of Benincasa hispida and Cucurbita moschata seeds by gas chromatography-mass spectrometry

“…Antibacterial activity [ 23 , 24 ]. 13-octadecenal(Z) and Hexadecanoic acid, 2-hydroxy-1- (hydroxyme) have antibacterial activities [ 25 ] and antioxidant properties respectively [ 22 ].…”

Bidens pilosa Linn.: Beautiful Weed for the Healthy Mouth – A Mini Review

“…[3, 13-19, 22, 38]. The alcoholic extracts inhibited the growth of Grampositive and Gram-negative bacteria [16,17,19,38]; even toward drug resistance bacteria [21], and Mycobacterium [17]; yeast, and fungi [3,16,17,19]. Other effects are also proven, including analgesic activity [18]; anti-inflammatory [3,13,14]; antioxidants [3,13,19,22]; antiplasmodial activity [3,15]; larvicidal activity [19]; haematopoietic strength and modulator of white blood cell differential count [3,12]; anticancer and antipyretic activity [3,19,20]; antiallergic activity, hypotensive effects, gastric antisecretory and antiulcer activities [3].…”

“…For antimicrobial purposes, it revealed that hydroethanolic extract were more potent compared to water extracts [38,75]. The extracts derived from leaves were reported active against wide range of microbes [17,19,38,29,49]. The B. pilosa extracts were also exhibited antifungal activity [16,17,19,49] and antiviral activity [32].…”

“…The properties are no longer folklores because many studies have been proven the activities in vitro, in vivo, and ex vivo assays [3,7,9,[12][13][14][15][16][17][18][19][20][21][22][23]. Currently, the plant become a market commodity, such as dried plant powder, B. pilosa tea, extracts, and tinctures.…”

Analytical Methods for Extraction and Identification of Primary and Secondary Metabolites of Apple (Malus domestica) Fruits: A Review

“…From a nutritional point of view, they contain a high number of sugars, balanced by the concentration of fibers [8]. In the last ten years, apples have been the subject of research and studies aiming to evaluate their effects on human health [9]. The daily intake of apples and apples-related products is often proposed in with weight control diets and has proved to have a statistically significant impact on weight reduction [10].…”