In vitro antibody response of human lymphocytes

Heijnen, Cobi J.; Uytdehaag, Fons G. C. M.; Ballieux, R. E.

doi:10.1007/bf00199926

Cited by 12 publications

(4 citation statements)

References 77 publications

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“…The Tpy-fraction is probably a rather heterogeneous pool of T-cells. It contains, apart from the precursor (target) suppressor cells (Heijnen et al, 1980), a precursor pool for the supply of Tp-cells, which can be detected after a concomitant interaction with various mitogenic stimuli and thus goes along with a simultaneous increase of Fc IgM receptor in up to 50% of the cells (Heijnen et aZ, 1981). In our experiments 68.4&12*9% of Tpylymphocytes were positive to the DAP IV reaction.…”

Section: Discussionmentioning

confidence: 99%

Enzymehistochemical staining of Tμ lymphocytes for glycyl-proline-4-methoxy-beta-naphthylamide-peptidase (DAP IV)

et al. 1982

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Section: Discussionmentioning

confidence: 99%

Enzymehistochemical staining of Tμ lymphocytes for glycyl-proline-4-methoxy-beta-naphthylamide-peptidase (DAP IV)

et al. 1982

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“…The identity of the in vitro generated T,-effector cells with the T3'8+y' suppressor-effector cell present in vivo is further supported by the observation that the former perform .their function via the production of a soluble suppressor factor ( [37] and manuscript in preparation), which is partly, if not completely, identical to the already described factor TSF120-0va [38] produced by T3' 8' y' blood cells [lo]. It should be emphasized, however, that the expression of the FcyR on the T cell is not a prerequisite for the capacity of the cell to exert T,-effector activity [23]. This assumption is supported by data depicted in Table 6, which show that Tar' cells, when activated for only 24 h (instead of 72 h) with primed Tp' cells, can already exert a T,-effector function, although no FcyR' T cells are detectable at this time.…”

Section: Discussionmentioning

confidence: 77%

Characterization of human T suppressor‐inducer, ‐precursor and ‐effector lymphocytes in the antigen‐specific plaque‐forming cell response

Heijnen

Pot

Ballieux³

1982

Eur J Immunol

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Suppression of an antigen-specific plaque-forming cell response of human blood lymphocytes can be effected by T mu+ cells that have been primed previously by antigen in vitro for 6 days. While lacking the capacity to suppress the plaque-forming response directly, these primed T mu+ suppressor-inducer cells stimulate a subpopulation of unprimed T mu gamma- cells to differentiate to T gamma + suppressor-effector cells. The T mu+, T gamma+ and T mu gamma- subsets have been shown to be heterogeneous populations of cells. Therefore, the functionally defined T suppressor-inducer, -precursor and -effector cells were characterized by OKT monoclonal antibodies and by the capacity to form rosettes with autologous erythrocytes (ar+). Evidence will be presented that in vitro a T4+mu+ar- cell induces a T8+mu gamma-ar+ precursor cell to differentiate to a T8+gamma+ar- suppressor-effector cell. A similar T suppressor-effector cell can also be isolated directly from peripheral blood of normal donors.

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“…In earlier investigations we established that the helper function in the antigeninduced PFC response resides in the T// subpopulation and is lacking in Ty* cells (Heijnen et al 1979a). Subsequent studies revealed that adequate helper activity could also be generated in the small subset of peripheral T lymphocytes, lacking receptors for IgM and IgG (Tfiy' cells), by stimulation with antigen in cell cultures (Table I) (Heijnen et al 1980), The T(iy'subset almost completely overlaps with the subset of peripheral blood T lymphocytes expressing affinity Tuy' cells isolated by depleting the total T cell fraction of Ifi and Ty* cells, or T//* cells isolated by rosetting with Eox-IgM complexes and subsequent density centrifugation, are cultured for 6 days in the presence of B cells, 5 % monocytes and 3//g OA/ml, When indicated ( t) the Tyuy" cells or the T/i* cells were irradiated with 1000 Rad, Cell numbersX IO' (n=3). for autologous erythrocytes (Tar*) (Heijnen et al 1982a); these T cells are considered to be postthymic precursor T lymphocytes (Palacios et al 1981).…”

Section: Helper/inducer T Cells: Functional and Phenotypical Heterogementioning

confidence: 99%

“…Whether the T/zy'ar* population contains mature T helper cells, lacking Fc//R, or contains T helper precursor lymphocytes is as yet unknown. The observation that Tiiy'a.r' cells, when activated by antigen, express Fc//R after only 5 h of culture, suggests that the Tar* population contains T helper precursor cells (Heijnen et al 1980). A further heterogeneity within the Tn* 'helper' population was encountered when Ifi* cells, isolated from unselected T cells, were primed by preculturing the cells with antigen for 5 or 6 days.…”

Section: Helper/inducer T Cells: Functional and Phenotypical Heterogementioning

confidence: 99%

Immunoregulatory T Cell Subpopulations in Man: Dissection by Monoclonal Antibodies and Fc‐Receptors

Ballieux¹,

Heijnen

1983

Immunological Reviews

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Until now, most of the studies on regulatory T cells have been based on culture systems in which human peripheral blood cells are stimulated by polyclonal stimulators like Pokeweed Mitogen (PWM). Our present contribution, however, deals with T cell-mediated regulation of the antigen-induced B cell activation, which exclusively leads to an antigen-specific IgM production (Heijnen et al. 1979a). Some authors' reports on regulatory activities of T cells, as tested in systems using polyclonal stimulators, differ from ours. This may be due to: a) as a result of polyclonal stimulus, various types of regulatory T cells are activated at the same time b) in contrast to a primary antigen, a polyclonal stimulator induces a rapid proliferation of the various regulatory T cells c) a polyclonal stimulator induces the differentiation of B cells in various maturational stages, that might each require additional or different regulatory signals. For example, Thomas et al. (1981) have shown that freshly isolated T4+ cells can induce suppressor activity in unprimed T8+ cells in the presence of PWM, whereas T4+ cells, precultured for 24 h in the presence of PWM, can exert a suppressor activity themselves without an apparent need for T8+ cells. In the antigen-specific system, however, we have neither been able to detect T suppressor effector activity in a population of primed T4+ cells, nor been able to demonstrate T suppressor inducer activity in unprimed T4+ cells (Heijnen et al. 1982a). Therefore the state of activation of the total T4+ population will dictate the balance of the total T helper and T suppressor activity. As a result of proliferation induced by polyclonal mitogens, small subsets of regulatory T cells, which are functionally undetectable in the primary antigen-specific assay, can expand sufficiently to have a measurable effect. Thomas et al. (1980) have shown that the T4+ suppressor inducer cell in the PWM system is radio-sensitive, which is in contrast with our data in the antigen-specific system. This may imply that we are looking at different subsets of T suppressor inducer cells in these different systems, but it might also indicate that T suppressor inducer cells need to proliferate in order to be able to measurably regulate the large pool of responding cells generated in the PWM system. Apart from such quantitative effects, polyclonal B cell activators like PWM are capable of inducing the differentiation of B cells in various maturational stages (Kuritani & Cooper 1982, Stevens 1982, Peters & Fauci 1983). Since it is highly likely that the regulation of these various B cell subsets might require different regulatory signals, the PWM model might be a very complicated model to study regulatory effects of single T cell subsets.(ABSTRACT TRUNCATED AT 400 WORDS)

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In vitro antibody response of human lymphocytes

Cited by 12 publications

References 77 publications

Enzymehistochemical staining of Tμ lymphocytes for glycyl-proline-4-methoxy-beta-naphthylamide-peptidase (DAP IV)

Enzymehistochemical staining of Tμ lymphocytes for glycyl-proline-4-methoxy-beta-naphthylamide-peptidase (DAP IV)

Characterization of human T suppressor‐inducer, ‐precursor and ‐effector lymphocytes in the antigen‐specific plaque‐forming cell response

Immunoregulatory T Cell Subpopulations in Man: Dissection by Monoclonal Antibodies and Fc‐Receptors

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