In vitro antidiabetic activities and GC-MS phytochemical analysis of Ximenia americana extracts

Shettar, Arun Kashivishwanath; Sateesh, M.K.; Kaliwal, B.B.; Vedamurthy, A. B.

doi:10.1016/j.sajb.2017.03.014

Cited by 47 publications

(25 citation statements)

References 30 publications

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“…Thus, given its wide geographical distribution and its biological potential, developing pharmaceutical formulations with X. americana is an innovative and promising proposal [ 8 , 15 , 17 , 18 ]. Within this context, the use of novel release forms as mucoadhesive tablets has resulted in a number of advantages over conventional formulations for treating oral infections, such as a longer residence time in the mucosa (saliva and mucosal movements remove conventional formulations quickly), increased permeability of the drug (against degrading agents present in the biological environment), and avoidance of the first-pass effect [ 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Tablet of Ximenia Americana L. Developed from Mucoadhesive Polymers for Future Use in Oral Treatment of Fungal Infections

et al. 2019

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The use of biocompatible polymers such as Hydroxypropylmethylcellulose (HPMC), Hydroxyethylcellulose (HEC), Carboxymethylcellulose (CMC), and Carbopol in solid formulations results in mucoadhesive systems capable of promoting the prolonged and localized release of Active Pharmaceutical Ingredients (APIs). This strategy represents a technological innovation that can be applied to improving the treatment of oral infections, such as oral candidiasis. Therefore, the aim of this study was to develop a tablet of Ximenia americana L. from mucoadhesive polymers for use in the treatment of oral candidiasis. An X. americana extract (MIC of 125 μg·mL−1) was obtained by turbolysis at 50% of ethanol, a level that demonstrated activity against Candida albicans. Differential Thermal Analysis and Fourier Transform Infrared Spectroscopy techniques allowed the choice of HPMC as a mucoadhesive agent, besides polyvinylpyrrolidone, magnesium stearate, and mannitol to integrate the formulation of X. americana. These excipients were granulated with an ethanolic solution 70% v/v at PVP 5%, and a mucoadhesive tablet was obtained by compression. Finally, mucoadhesive strength was evaluated, and the results demonstrated good mucoadhesive forces in mucin disk and pig buccal mucosa. Therefore, the study allowed a new alternative to be developed for the treatment of buccal candidiasis, one which overcomes the inconveniences of common treatments, costs little, and facilitates patients’ adhesion.

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Section: Introductionmentioning

confidence: 99%

Tablet of Ximenia Americana L. Developed from Mucoadhesive Polymers for Future Use in Oral Treatment of Fungal Infections

et al. 2019

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“…This compound has been reported to have antimicrobial and antioxidant activity as well as used in manufacture of detergents [44,42]. 1-Tridecene is a plant metabolized fatty acid which has been previously reported to have remarkable antibacterial activity as well as use in treatment of respiratory irritation in human beings [45]. The compound 1-Tridecene has been identified using GC-MS in Solanaceae species such as Solanum spirale and S. incanum [46].…”

Section: Gc-ms Results Of Solanum Incanummentioning

confidence: 99%

Phytochemical Composition and Antibacterial Activity of Fruit Extract of Solanum incanum L. against Ralstonia solanacearum

Karanja

K’Owino

Wangila

et al. 2021

AJACR

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Aims: To determine the phytochemical composition and antibacterial activity of Solanum incanum fruits against Ralstonia solanacearum. Study Design: Experimental design involving completely randomized design Place and Duration of Study: The study was conducted at department of Chemistry and Biochemistry, School of Sciences and Aerospace studies, Moi University, Kenya, between January and June 2021. Methodology: Extraction was done by maceration using ethanol as the extracting solvent. Phytochemical screening was done following standard procedures. Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) were determined using the Folin–Ciocalteu colorimetric method and aluminum chloride colorimetric assay respectively. The extract was further analyzed using Gas Chromatography Mass spectroscopy (GC-MS) and Fourier transformed Infrared (FT-IR). In vitro antibacterial activity was determined using disc diffusion method while in vivo studies was done under greenhouse conditions. Results: Phytochemical analysis showed presence of alkaloids, glycosides, steroids, tannins, flavonoids, phenols, saponins and terpenoids. The TPC and TFC were found to be 84.997 ± 0.2 mg GAE/g and 20.535 ± 0.2 mg/g QE of dried sample respectively. GC-MS analysis revealed the presence of 15 compounds, (9E)-1-Methoxy-9-Octadecene (26.85%), 9-Octadecenamide (Z) (21.43%), E-15-Heptadecenal (7.28%), E-14-Hexadecenal (6.28%), 2,4-Di-tert-butylphenol (4.96%) among others. FT-IR analysis revealed presence of OH, C-H, N-H, CO functional groups at wavenumbers 3348 cm-1, 2931 cm-1, 1589 cm-1, and 1218 cm-1 respectively. The antibacterial activity for in vitro studies at concentrations 0.01, 0.05, 0.10, and 0.15 g/10 mL, the diameters of zone of inhibition were 20.75 ± 1.3, 25.75 ± 0.5, 27.25 ± 0.5, and 30.75 ± 0.5 mm respectively. This was comparable (P= .02) to that of ampicillin (positive control) which had zones of inhibition of 26.75 ± 0.5, 28.75 ± 0.5, 31.75 ± 0.4, and 35.00 ± 0.0 mm at the concentrations respectively. For the in vivo studies the plant extract and ampicillin delayed the development of the disease by eight and ten days post-inoculation respectively while symptoms of bacterial wilt for water treatment (negative control) were observed four days post-inoculation. Conclusion: The plant extract had remarkable antibacterial activity and can be used to make viable formulations to control the devastating bacterial wilt disease.

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“…The distinct method of Shettar et al [2] was followed to determine the glucose uptake in yeast cells. 1% suspension of commercially available baker's yeast (Saccharomyces cerevisiae) cells was set at 25°C.…”

Section: Determination Of In Vitro Antidiabetic Activity By Glucose Uptake In Yeast Cell Modelmentioning

confidence: 99%

“…[1] Among them, diabetes mellitus (DM) is considered as a 7th leading source of death in 2030 as per WHO (World Health Organization) reports. [2] The defective insulin action or insulin secretion ensue the rise of blood glucose levels in the body by hampering the uptake of glucose into the cells leading to a variety of…”

mentioning

confidence: 99%

In Vitro Antidiabetic Activity by Glucose Uptake of Yeast Cell Assay and Antioxidant Potential of Annona Reticulata L. Leaf Extracts

Pulivarthi

Penchalaneni

Naidu

2020

Int. J. Pharm. Sci. Drug Res.

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The undesirable adverse effects of present available synthetic drugs endorse the modern medicine to search the superior choice for the treatment of metabolic diseases. Herbal medicine turns out to be a hopeful therapy for the effective treatment of diabetes in foreseeable future. In an effort to render a scientific evidence for the antidiabetic potential of Annona reticulata L., the present research, with the objective to evaluate the ability of A. reticulata leaf extracts on antihyperglycemic property under in vitro using yeast cell model was performed. Besides, as the diabetes and its complications are highly associated with the oxidative stress, the current study was also focussed on antioxidant property of A. reticulata leaf extracts. The scavenging ability of plant extracts on free radical 2, 2 – diphenyl- 1- picrylhydrazyl (DPPH), ferric reducing power assay and total antioxidant activity were performed to establish the antioxidant potential of A. reticulata leaf extracts. The in vitro antidiabetic ability of A. reticulata leaves was evaluated by glucose uptake method using yeast cell model. Among the four chosen solvent extracts- aqueous, methanol, ethyl acetate and n-hexane, methanol extract (MeE) exhibited high antioxidant potential. MeE exhibited 62.28% of DPPH inhibition at 200µg/ml, with total antioxidant activity 164.72 ± 2.63µg/ml and higher absorbance in reducing power assay (1.15 ± 0.03). In vitro antidiabetic activity by glucose uptake of yeast cell assay was evaluated and observed dose dependant rise in % of glucose uptake in methanol, ethylacetate and aqueous extracts of A. reticulata. MeE showed 48.55% of glucose uptake at 500µg/ml concentration. Hence the present study could be concluded as A.reticulata leaves possess potent antioxidant activity and antidiabetic activity under in vitro conditions. With the outcome of the present initial study, research work could be extended further; thereby the exact pharmacological action of the plant compounds could be discovered.

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In vitro antidiabetic activities and GC-MS phytochemical analysis of Ximenia americana extracts

Cited by 47 publications

References 30 publications

Tablet of Ximenia Americana L. Developed from Mucoadhesive Polymers for Future Use in Oral Treatment of Fungal Infections

Tablet of Ximenia Americana L. Developed from Mucoadhesive Polymers for Future Use in Oral Treatment of Fungal Infections

Phytochemical Composition and Antibacterial Activity of Fruit Extract of Solanum incanum L. against Ralstonia solanacearum

In Vitro Antidiabetic Activity by Glucose Uptake of Yeast Cell Assay and Antioxidant Potential of Annona Reticulata L. Leaf Extracts

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