In Vitro Antidiabetic Activity and Phytochemical Analysis of Lumnitzera Racemosa Leaves

Abstract

The present study was designed to evaluate the antidiabetic property and phytochemical composition of Lumnitzera racemosa leaves. Mangrove plant L. racemosa was collected in the month of May from Bhatye beach area Ratnagiri coast, Maharashtra, India. Leaves separated, powdered and exhaustive extraction was carried out in methanol, ethanol, ethyl acetate, and pet ether solvents with increasing polarity using Soxhlet apparatus and in vitro antidiabetic activity was determined by α-amylase and α-glucosidase inhibitory assays using acarbose as standard. Phytochemical analysis was carried out as per Wagner and Bladt (1996). Results have confirmed antidiabetic activity in methanol extract which was comparable to standard acarbose followed by ethyl acetate, ethanol and pet ether extracts. The phytochemical results showed the presence of flavonoid, saponin, essential oil, alkaloid, anthraquinones, bitter principles, phenol, triterpenoids, coumarin, and tannin in the leaves.

Antidiabetic Activity of an Alkaloid (4a-Methyl-5-(6-Methylhept- 5-En-1-Yl)Octahydro-1H-Cyclopenta[A]Pyridazine) Isolated From Lumnitzera racemosa in Streptozotocin-Induced Diabetic Wistar Rats

“…In our earlier study, we found L. racemosa to be a potent inhibitor of α-amylase and α-glucosidase enzymes in vitro and a rich source of secondary metabolites like alkaloid, flavonoid, and saponin as well which are proved to possess antidiabetic activity. 12 So in continuation of our previous work, the present study has been undertaken to isolate an antidiabetic principle from L. racemosa leaves and evaluate its antidiabetic efficacy in vivo on Streptozotocin-induced Wistar rats. Thus, a bioactive compound was isolated from L. racemosa which is characterized as an alkaloid, named 4a-methyl-5-(6-methylhept-5-en-1-yl)octahydro-1Hcyclopenta[a] pyridazine (MOCP).…”

“…All three fractions were tested for their in vitro antidiabetic activity, which was estimated by α-amylase and α-glucosidase inhibition assays by following the method mentioned in our previous report. 12…”

“…This plant has antibacterial, 9 hepatoprotective, antioxidant, 10 cytotoxic, 11 and antidiabetic activities. 12 Based on our in vitro antidiabetic activity of L. racemosa leaves, 12 the present study's main objective was to isolate the antidiabetic principle from L. racemosa leaves and to check its antidiabetic efficacy in vivo.…”