In vitro antidrepanocytary actvity (anti-sickle cell anemia) of some congolese plants

Mpiana, P. T.; Tshibangu, Damien S. T.; ., O.M. Shetonde; Ngbolua, Koto-te-Nyiwa

doi:10.1016/j.phymed.2006.05.008

Cited by 94 publications

(75 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of them exhibited anti-sickling properties in vitro. These include Piper guineensis, Pterocarpa osun, Eugenia caryophyllala and Sorghum bicolor [21]; Fagara zanthoxyloides [22]; Cajanus cajan [23]; Vigna unguiculata [24]; Carica papaya [25,26]; and Justicia secunda ( [27,28].…”

Section: Management Of Sickle Cell Disease Using Medicinal Plantsmentioning

confidence: 99%

Structure Activity Relationship (SAR) of Some Benzoic Acid Derivatives from Plant Origin that Exhibit Anti-Sickling Properties in vitro - (Review)

Pierre¹,

Moses²,

Peter³

2015

Nat Prod Chem Res 2015

View full text Add to dashboard Cite

Section: Management Of Sickle Cell Disease Using Medicinal Plantsmentioning

confidence: 99%

Structure Activity Relationship (SAR) of Some Benzoic Acid Derivatives from Plant Origin that Exhibit Anti-Sickling Properties in vitro - (Review)

Pierre¹,

Moses²,

Peter³

2015

Nat Prod Chem Res 2015

View full text Add to dashboard Cite

“…Spices, diuretic, anthelmintic, fever, antiparasitic, stomach troubles; diarrhoea, vermifuges (Abreu and Noronha, 1997;Neuwinger, 2000) Fruits Antimicrobial, antitumor and antileishmania, antidrepanocytary, Anthelmintic (Abreu et al, 1999;Mpiana et al, 2007) Aframomum kayserianum K.schum (Zingiberaceae) 18884/SRFC / Amomum kayserianum Fruits Spices, anti-mumps, diarrhoea, dysmenorrhoeas, vermifuge (Tane et al, 2005).…”

Section: Roots Fruitsmentioning

confidence: 99%

The 15-lipoxygenase inhibitory, antioxidant, antimycobacterial activity and cytotoxicity of fourteen ethnomedicinally used African spices and culinary herbs

Dzoyem

Kuete

McGaw

et al. 2014

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Ethnopharmacological relevanceCulinary herbs and spices are widely used ethnomedically across Africa. They are traditionally employed in the treatment of several ailments including inflammation disorders, pain alleviation and infectious diseases. Pharmacological studies are necessary to provide a scientific basis to substantiate their traditional use and safety. In this study, the 15-lipoxygenase inhibitory, antioxidant, antimycobacterial and the cytotoxic activities, total phenolic and flavonoid contents of fourteen edible plants were investigated. Materials and MethodsThe 15-lipoxygenase inhibitory activity was evaluated by the ferrous oxidation-xylenol orange (FOX) assay method. The antioxidant activity was determined using free-radical scavenging assays. The antimycobacterial activity was determined by a broth microdilution method against three species of mycobacteria: Mycobacterium smegmatis, Mycobacterium aurum and Mycobacterium fortuitum using tetrazolium violet as growth indicator. The cytotoxicity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on Vero monkey kidney cells. ResultsAll the extracts tested had some 15-lipoxygenase inhibitory activity ranging from 32.9-78.64%.Adansonia digitata (fruit) had the highest antioxidant capacity (IC 50 values of 8.15 μg/mL and 9.16 μg/mL in the DPPH and ABTS assays respectively; TEAC of 0.75 in the FRAP assay) along with the highest amount of total phenolics (237.68 mg GAE/g) and total flavonoids (16.14 mg QE/g). There were good correlations between DPPH and ABTS values (R 2 0.98) and between total phenolics and total flavonoids (R 2 0.94). Tamarindus indica had significant antimycobacterial activity against M. aurum (MIC 78 μg/mL). As could be expected with edible plants, all the extracts had a relatively low cytotoxicity with LC 50 values higher than 102 μg/mL with the exception of the two Aframomum species (33 and 74 μg/mL). 2 ConclusionsThis study provides scientific support for some of the the traditional uses and the pharmacological activities of the culinary herbs and spices investigated. The results suggest that increasing intake of some of these herbs may be useful in preventing or reducing the progression of lifestyle-related diseases. The diversity of the pharmacological activities of the extract from the fruit of Adansonia digitata suggested that this plant might be valuable for application in human and animal health.

show abstract

“…The consequences of this defect are hemolytic anemia and tissue damage brought about by the blockage of blood vessels by the sickled cells. The complications can be severe and include retarded growth, periodic attacks of pain and progressive organ dysfunction leading in most cases to a much reduced life expectancy [3]. Each year about 300,000 children are born with pathological hemoglobin of which 70% are affected by SCD.…”

Section: Introductionmentioning

confidence: 99%

“…Most of them die before the age of five years when they do not receive regular medical care. In Democratic Republic of the Congo, almost 2% of the population is sicklers [3][4][5]. The first-line clinical management of SCD includes medullar transplantation, repeated blood transfusion to stabilize the patient's hemoglobin level, and the use of chemical agents which interfere with the mechanism and/or kinetics of the sickling process.…”

Section: Introductionmentioning

confidence: 99%

Ethno-Pharmacological Survey, In Vitro Anti-Sickling and Free Radical Scavenging Activities of Carapa Procera DC. Stem Bark (Meliaceae)

Ngbolua¹,

Bishola²,

Mpiana³

et al. 2014

nova-jmbs

View full text Add to dashboard Cite

Drepanocytosis is a genetic and neglected disease, endemic in negroids population. One of the main characteristics of this pathology is the production of a large amount of free radicals, leading to a severe oxidative stress and the consumption of NO by free oxygen radicals, and/or by cell-free plasma heme. The consequences of this defect are hemolytic anemia and tissue damage brought about by the blockage of blood vessels by the sickled cells. The present study evaluated the antisickling and radical scavenging activities of extracts from Carapa procera stem bark using Emmel's test and the DPPH assay. Carapa procera was selected through an ethnopharmacological. The results showed that methanolic, ethyl acetate and dichloromethane soluble fractions, anthocyanins and organic acids exhibited a significant antisickling as revealed by the observed normal biconcave form of sickle erythrocyte (normalization rate > 70%) in hypoxic conditions. Methanolic extract exhibited a good radical scavenging activity (ED50 = 1.698 ± 0.079 µg/mL). The chemical screening performed on the plant revealed the presence of anthocyanins and organic acids which were then extracted. Total anthocyanins and organic acids revealed interesting antisickling and antioxidant properties that could justify the integration of Carapa procera in Congolese pharmacopoeia for the management of sickle cell disease. Bioactive extracts from this plant species could increase nitric oxide by scavenging free oxygen radicals. For the best our knowledge, Carapa procera has not been yet previously reported as antisickling plant in the traditional medicine database of Democratic Republic of the Congo.

show abstract

In vitro antidrepanocytary actvity (anti-sickle cell anemia) of some congolese plants

Cited by 94 publications

References 2 publications

Structure Activity Relationship (SAR) of Some Benzoic Acid Derivatives from Plant Origin that Exhibit Anti-Sickling Properties in vitro - (Review)

Structure Activity Relationship (SAR) of Some Benzoic Acid Derivatives from Plant Origin that Exhibit Anti-Sickling Properties in vitro - (Review)

The 15-lipoxygenase inhibitory, antioxidant, antimycobacterial activity and cytotoxicity of fourteen ethnomedicinally used African spices and culinary herbs

Ethno-Pharmacological Survey, In Vitro Anti-Sickling and Free Radical Scavenging Activities of Carapa Procera DC. Stem Bark (Meliaceae)

Contact Info

Product

Resources

About