In Vitro Antifungal Activity of <i>Satureja horvatii</i> ssp. <i>macrophylla</i> Against 3 Tomato Phytopathogenic Fungi

Yfanti, Paraskevi; Patakioutas, G.; Douma, Dimitra; Lekka, Marilena E.

doi:10.1177/1934578x211025165

Cited by 5 publications

(2 citation statements)

References 33 publications

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“…The essential oils, isolated from the aromatic plants, were analyzed by a gas chromatograph (GC) coupled with a mass spectrometer (MS) (GC-2030, GCMS-QPSERIES, Shimadzu, Kyoto, Japan). The essential oil constituents were separated using a Mega 5-MS capillary column (30 m × 0.25 mm × 0.25 μm), helium as carrier gas with a flow rate of 0.9 mL min −1 and a temperature program, previously described [ 36 ]. For the analysis, 1 μL of the essential oil was diluted in n-hexane (1:200) and then injected by autosampler (AOC-20i/s Shimadzu), while the injector was set at split mode (split ratio: 30).…”

Section: Methodsmentioning

confidence: 99%

Enrichment of Olive Oils with Natural Bioactive Compounds from Aromatic and Medicinal Herbs: Phytochemical Analysis and Antioxidant Potential

Yfanti,

Lazaridou,

Boti

et al. 2024

Molecules

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Olive oil and herbs, two key components of the Mediterranean diet, are known for their beneficial effects on humans. In our study, we incorporated aromatic and medicinal herbs into local monovarietal olive oils via maceration procedures for enrichment. We identified the herbal-derived ingredients that migrate to olive oils and contribute positively to their total phenolic content and functional properties, such as radical scavenging activity. Thus, we characterized the essential oil composition of the aromatic herbs (GC-MS), and we determined the phenolic content and antioxidant capacity of the additives and the virgin olive oils before and after enrichment. The herbal phenolic compounds were analyzed by LC-LTQ/Orbitrap HRMS. We found that olive oils infused with Origanum vulgare ssp. hirtum, Rosmarinus officinalis and Salvia triloba obtained an increased phenolic content, by approximately 1.3 to 3.4 times, in comparison with the untreated ones. Infusion with S. triloba led to a significantly higher antioxidant capacity. Rosmarinic acid, as well as phenolic glucosides, identified in the aromatic herbs, were not incorporated into olive oils due to their high polarity. In contrast, phenolic aglycones and diterpenes from R. officinalis and S. triloba migrated to the enriched olive oils, leading to a significant increase in their phenolic content and to an improvement in their free radical scavenging capacity.

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Section: Methodsmentioning

confidence: 99%

Enrichment of Olive Oils with Natural Bioactive Compounds from Aromatic and Medicinal Herbs: Phytochemical Analysis and Antioxidant Potential

Yfanti,

Lazaridou,

Boti

et al. 2024

Molecules

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“…This Ascomycota causes signi cant economic losses pre-and post-harvest when climatic conditions are favorable (i.e., low temperatures and humid conditions) (Bahammou et al, 2017). The symptoms of gray mold disease include velvety, gray-brown mold on stems and leaves and soft rot with whitish "ghost spots" (small 3-8 mm rings) on the fruit surface that lead to serious damage during all development stages through storage and result in regrettable losses in total yields (Yfanti et al, 2021). Moreover, the disease is responsible for approximately $10-100 million in economic losses in tomato production worldwide annually, decreasing yields by as much as 60% ( Several control approaches based on the integration of various management strategies for this airborne fungus have been documented, including efforts to prevent appressorium formation and inhibit spore germination (Bahammou et al, 2017;Li and Zou, 2017).…”

Section: Introductionmentioning

confidence: 99%

Valorization of Capsicum annuum seed extract as an antifungal against Botrytis cinerea

Hajji-Hedfi,

Rhouma,

Al-Judaibi

et al. 2023

Waste Biomass Valor

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Botrytis cinerea Pers., the causal agent of gray mold, is an airborne pathogen that causes signi cant damage to tomato crops worldwide at all development stages and post-harvest. In this study, the aqueous extract of Capsicum annuum seeds was screened for its phytochemical constituents and assessed at various concentrations (10, 20, 30, and 60%) for antifungal activity in vitro. Selected biochemical, pathological, agronomical, physicochemical, and morphometrical traits were investigated to determine the effectiveness of applying the aqueous seed extract and salicylic acid either separately or in combination to tomato seeds and fruits in vivo. Phytochemical screening of the aqueous seed extract showed the presence of 2, 2-diphenyl-1picrylhydrazyl, phenolic and avonoid contents, quinic acid, protocatechuic acid, syringic acid, p-coumaric acid, trans-ferulic acid, rutin, quercetin-3-o-rhamonosic, kaempferol, naringenin, and apigenin at various concentrations. The ndings suggested that the aqueous extract at a concentration of 60% was most e cient in vitro where mycelial growth was < 3.8 mm, mycelial growth inhibition was > 52%, and mycelial growth rate of < 1.05 mm/h. In vivo, the combined treatments of tomato seeds produced the greatest reduction in gray mold damage (disease severity index 8.67%) and the most favorable growth parameters of seedlings were chlorophyll a > 1.50 mg/g.f.Wt.; chlorophyll b > 1.76 mg/g.f. Wt.; total chlorophyll content > 3.26 mg/g.f.Wt.; seedling fresh weight > 0.43 g; seedling length > 12.43 cm, respectively. Combined preventive treatment applied to tomato fruits inoculated with B. cinerea resulted in the lowest disease severity (percentage of fruit area covered by gray mold < 33.33%; disease severity index < 46.67%) and the most favorable physicochemical attributes (water content < 98.28%; juice yield > 53.35%; pH < 3.59; titratable acidity > 1.37 g/10 ml juice; Brix degree > 4.73; nitrate content < 383.33 mg/kg; electrical conductivity < 2.47 mS/cm) and morphometrical attributes (fruit rmness > 3.03). The combined treatments resulted in the strongest activity of peroxidase (> 4.162 units/mg/min), ascorbate peroxidase (> 31.66 µmol/mg/min), and malondialdehyde (> 3.90 µmol/g) on the tomato fruits.The aqueous extract of C. annuum seeds combined with salicylic acid had positive effects in terms of inhibiting B. cinerea and is thus a promising and environmentally friendly alternative substitute for chemical fungicides towards sustainable agriculture under climate change. Statement of noveltyCapsicum annuum seeds extracts inhibited signi cantly the growth rate of B. cinerea. Combining salicylic acid with the seed extracts reduced the fruit gray mold disease severity. The seed extracts can improve the tomato fruit's biochemical proprieties and enhance some ROS enzymes. The use of C. annum seeds as bio-fungicide origin is innovative and promising tool to control the gray mold disease of tomato.

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Novel nanoemulsion-loaded hydroxyl propyl methyl cellulose films as bioactive food packaging materials containing Satureja khuzestanica essential oil

Aghajani,

Rafati,

Aliahmadi

et al. 2024

Carbohydrate Polymer Technologies and Applications

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In Vitro Antifungal Activity of Satureja horvatii ssp. macrophylla Against 3 Tomato Phytopathogenic Fungi

Cited by 5 publications

References 33 publications

Enrichment of Olive Oils with Natural Bioactive Compounds from Aromatic and Medicinal Herbs: Phytochemical Analysis and Antioxidant Potential

Enrichment of Olive Oils with Natural Bioactive Compounds from Aromatic and Medicinal Herbs: Phytochemical Analysis and Antioxidant Potential

Valorization of Capsicum annuum seed extract as an antifungal against Botrytis cinerea

Novel nanoemulsion-loaded hydroxyl propyl methyl cellulose films as bioactive food packaging materials containing Satureja khuzestanica essential oil

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