In vitro antifungal activity of polyphenols-rich plant extracts against Phytophthora cinnamomi Rands

Castillo, Francisco Daniel Hernández; HernÃ¡ndez-Castillo, Francisco Daniel; Clemente-Constantino, Julio Alberto; Gallegos‐Morales, Gabriel; RodrÃ­guez-Herrera, RaÃol; Aguilar, CristÃ3bal NoÃ©

doi:10.5897/ajar2013.8072

Cited by 18 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, these emulsions inhibited the mycelium growth of R. solani at concentrations of 1.70 × 10 4 and 6.72 × 10 3 mg/L, respectively. Similarly, Francisco et al [119] confirmed the antifungal activity of lanolin extract of this species against P. cinnamomi at IC 50 of 23.07 mg/mL. In contrast, lanolin extract exhibited an antifungal concentration lower than ethanolic extract but similarly to aqueous extract (28.87 mg/mL), which suggested that lanolin emulsions can also be efficient solvents to recover bioactive compounds.…”

Section: Antifungal Activitymentioning

confidence: 81%

Hunting Bioactive Molecules from the Agave Genus: An Update on Extraction and Biological Potential

et al. 2021

View full text Add to dashboard Cite

Agaves are plants used in the production of alcoholic beverages and fibers. Ever since ancient times, pre-Hispanic cultures in Mexico have used them in traditional medicine to cure different ailments. Over the years, studies of the active principles responsible for the therapeutic benefits of agaves have increased. Leaves and fibers are the main agro-wastes generated in tequila and mezcal production, while fibers are the main waste product in the textile sector. Different investigations have referred to the agro-waste from agave processing as a source of bioactive molecules called secondary metabolites (SM). Among them, phenols, flavonoids, phytosterols, and saponins have been extracted, identified, and isolated from these plants. The role of these molecules in pest control and the prospect of metabolites with the biological potential to develop novel drugs for chronic and acute diseases represent new opportunities to add value to these agro-wastes. This review aims to update the biological activities and recent applications of the secondary metabolites of the genus Agave.

show abstract

Section: Antifungal Activitymentioning

confidence: 81%

Hunting Bioactive Molecules from the Agave Genus: An Update on Extraction and Biological Potential

et al. 2021

View full text Add to dashboard Cite

show abstract

“…(Munguía et al, 2014). Francisco et al (2015) assessed the in vitro antifungal activity of L. tridentata water, ethanol, lanolin, and cocoa butter extracts against P. cinnamomi, the ethanol extract completely inhibited mycelium, while the lanolin extract showed very low inhibition. L. tridentata ethanol and dichloromethane extracts reduced fungal growth by 75-100% against A. tenuissima, A. niger, Penicillium polonicum, and Rhizopus oryzae.…”

Section: Introductionmentioning

confidence: 99%

Polyphenolic Bioactive Compounds from Larrea tridentata (DC.) Coville: Extraction, Characterization, Antioxidant, and Antifungal Activities

Bamidele,

Pérez,

Sandoval-Cortes

et al. 2024

Preprint

View full text Add to dashboard Cite

The significance of medicinal plants in inhibiting microbial growth in food and agricultural production as well as their economic viability cannot be overstated. These plants contain secondary metabolites, which are abundant in antimicrobial compounds, such as flavonoids, tannins, saponins, and alkaloids, and their extracts have demonstrated antimicrobial properties against a variety of plant pathogens. The primary objective of this study was to explore the possibility of using bioactive compounds in plant defenses and their biological applications. To achieve this, antifungal polyphenolic bioactive compounds were extracted from the stems and leaves of L. tridentata using conventional methods. The total polyphenol and antioxidant potential of the extracts were assessed and characterized using high-performance liquid chromatography (HPLC). This study compared the polyphenolic constituents of extracts from emerging maceration and Soxhlet extraction techniques in the leaves and stems of Larrea tridentata. The extracts were evaluated for total polyphenolic content (hydrolyzable (HT) and condensed tannins (CT)) and antioxidant activity (ABTS, FRAP, and DPPH). Reverse-Phase High-Performance Liquid Chromatography Electrospray Ionization coupled with mass spectrometry (RP-HPLC-ESI-MS) was used for qualitative identification of antimicrobial phytochemicals. Furthermore, the extracts were analyzed in vitro for antifungal activity against Fusarium oxysporum and Alternaria alternata. The results revealed that 60:40 ethanol:water macerated leaf extract gave the highest hydrolysable tannins (6.41 ± 0.08 mg GAE/g), while its equivalent showed the highest condensed tannins (2.81 mg CE/g). Soxhlet ethyl acetate leaf (SOX ELL) extract showed 1.14 times more condensed tannin content than that of the stems. The antioxidant potential of the extract increased with increasing polarity of the extraction solvent. SOX ELL had higher antifungal effects against F. oxysporum and A. alternata, whereas the 60:40 ethanol: water ratio resulted in 52% inhibition against A. alternata and 43% inhibition against F. oxysporum. Polyphenols with antifungal properties were found in the extracts, including caffeic acid 4-O-glucoside, rhamnetin, protocatechuic acid 4-O-glucoside, kaempferol, (+)-gallocatechin, luteolin, guteolin 7-O-(2-apiosyl-glucoside), gallic acid 4-O-glucoside, cumaric acid 4-O-glucoside, quercetin, NDGA, piceatannol 3-O-glucoside, pterostilbene, tetramethylscutellarein, and cirsimaritin. L. tridentata leaf extracts exhibit potential effectiveness in the development of biological control agents, which can not only enhance crop protection, but also contribute to overall agricultural sustainability.

show abstract

Interactive effects of biotic stressors and provenance on chemical defence induction by holm oak (Quercus ilex)

Rodríguez-Romero

Gallardo

Pérez

et al. 2021

Trees

View full text Add to dashboard Cite

Key Message The patterns of induced chemical defences in Quercus ilex leaves are specific to the biotic stress factor that causes them. Interactive effects between stressors depend on provenance. Abstract Quercus forests are suffering serious decline worldwide, closely linked to the consequences of climate change. The increase of biotic stressors threatens the survival of the holm oak (Quercus ilex), a dominant tree species in the Mediterranean Basin. A better understanding of its resistance mechanisms is urgently required to enable a better control of its decline. In this work, the ability of holm oaks from six Iberian provenances to respond to multiple biotic damage is studied through an analysis of their induced chemical defence patterns. Using 2016 seedlings established in a common garden trial (6 regions × 12 families/region × 7 seedlings/family × 4 treatments), biotic damage was induced at the root level (by infection with the widespread pathogen Phytophthora cinnamomi) and at the above-ground level (by mechanical defoliation). The levels of constitutive and induced total phenols, total tannins and condensed tannins were measured. Results showed that (1) the defensive chemical patterns present significant local and geographical variation, (2) survival to stress is more related to constitutive defences than induced ones, (3) the induced response is stressor-specific, and (4) there is an interactive effect amongst stressors whose sign (induction/inhibition) depends on the provenance. These findings on biotic stressor effects on the chemical defences and survival of holm oak can contribute to the development of genetic material selection programs in the integrated control of the widespread decline of Quercus.

show abstract

In vitro antifungal activity of polyphenols-rich plant extracts against Phytophthora cinnamomi Rands

Cited by 18 publications

References 15 publications

Hunting Bioactive Molecules from the Agave Genus: An Update on Extraction and Biological Potential

Hunting Bioactive Molecules from the Agave Genus: An Update on Extraction and Biological Potential

Polyphenolic Bioactive Compounds from Larrea tridentata (DC.) Coville: Extraction, Characterization, Antioxidant, and Antifungal Activities

Interactive effects of biotic stressors and provenance on chemical defence induction by holm oak (Quercus ilex)

Contact Info

Product

Resources

About