In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum

Abstract

a b s t r a c tFood spoilage caused by mycotoxigenic moulds represents an important problem in food security. The antimicrobial peptides are compounds of natural origin constituted by a variable number (5e100) of amino acids held together through peptide bonds. In this work, the cell free supernatants (CFSs) containing peptides obtained from four strains of LAB were lyophilized, filtered and tested to determine the antifungal activity against Aspergillus Parasiticus and Penicillium expansum. CFS obtained by Lactobacillus plantarum showed the highest inhibition activity. CFS was fractionated by size exclusion chromatography and injected into the liquid chromatography coupled to diode array detector. One of the recollected fractions resulted interesting for the presence of three peaks that were purified by the technique of the LC-DAD using a semi preparative C18 column. Finally, the antifungal activity of the purified peptides was studied against A. Parasiticus and P. expansum in liquid medium. The MALDI-TOF/TOF mass spectrometry was used for the peptides identification. The three purified peptides presented an amino acidic sequence identified by a bioinformatics program of SGADTTFLTK, LVGKKVQTF, and GTLIGQDYK. The first peptide purified reduced 58% and 73% the growth of P. expansum and A. parasiticus, respectively, in liquid medium after 48 h incubation.

Lactic Acid Bacteria as Antifungal and Anti‐Mycotoxigenic Agents: A Comprehensive Review

“…The analysis of the cell‐free supernatant (CFS) revealed a novel protein of 1256.6 kDa being responsible for this activity, the mechanism of action of which remained unknown (Rather et al., ). Similarly, L. plantarum strains have recently been reported to produce anti‐fungal bioactive peptides against Aspergillus parasiticus and P. expansum (Luz, Saladino, Luciano, Mañes, & Meca, ). L. plantarum strains have also shown strong antifungal activity against Aspergillus fumigatus , Botrytis elliptica (Ilavenil et al., ), Rhodotorula mucilaginosa , P. brevicompactum (Lačanin et al., ), Trichothecium roseum (Lv et al., ), Mucor circinelloides , Fusarium verticillioides (Quattrini, Bernardi et al., ), Eurotium rubrum (Muhialdin, Hassan, Abu Bakar, & Saari, ), Rhizopus stolonifer , Mucor racemosus (Gupta & Srivastava, ), Sclerotinia minor (Sathe et al., ), Rhizopus nigricans , and Penicillium oxalicum (Zhang et al., ).…”

“…There has been great progress in understanding and exploring novel antifungal metabolites of LAB during the last few years. Apart from some novel antifungal peptides produced by many LAB species (Gerez, Torres, Font de Valdez, & Rollán, 2013;Luz et al, 2017;McNair et al, 2018;Muhialdin et al, 2016;Muhialdin, Hassan, & Saari, 2018;Rather et al, 2013), many other novel antifungal metabolites of LAB have been reported recently that included 10-hydroxy-12-octadecenoic acid (10-HOE), 13-hydroxy-9-octadecenoic acid (Chen, Liang, Curtis, & Ganzle, 2016), benzeneacetic acid, 2-propenyl ester (Wang, Yan, Wang, Zhang, & Qi, 2012), phenolic antioxidants (such as, 2,4 di-tert-butyl phenol) (Sellamani et al, 2016;Varsha et al, 2015), 2-hydroxy-(4-methylthio) butanoic acid, 2-hydroxy-3-methylbutanoic (Honoré et al, 2016) and spermine-like and short cyclic polylactates (Mosbah et al, 2018). Aunsbjerg et al (2015) reported the role of volatile compounds (diacetyl) produced by Lactobacillus paracasei DGCC 2132 in inhibiting the growth of two fungal species belonging to the genus Penicillium: P. solitum DCS 302 and Penicillium sp.…”

Control of Aflatoxigenic Molds by Antagonistic Microorganisms: Inhibitory Behaviors, Bioactive Compounds, Related Mechanisms, and Influencing Factors

“…In another study of Ahlberg et al [26], 171 LAB strains were tested against A. flavus, and the species with the highest antifungal ability was identified as L. plantarum. The genus Lactobacillus, mainly the species L. plantarum, has been widely found to inhibit aflatoxigenic strains in various living environments [22,[58][59][60][61]. LAB strains are mainly divided into four genera: Lactobacillus, Lactococcus, Pediococcus, and Leuconostoc.…”

Bioactive peptides and proteins as alternative antiplatelet drugs

“…Peptides have recently gained significant attention as potential therapeutic agents for treating a plethora of ailments . Over 7000 naturally‐occurring peptides have essential roles in modifying human pathophysiology, falling under groups such as growth factors, anti‐infective agents, hormones, ion channel ligands, and neurotransmitters .…”

“…Peptides have recently gained significant attention as potential therapeutic agents for treating a plethora of ailments. [39][40][41][42][43][44][45][46] Over 7000 naturally-occurring peptides have essential roles in modifying human pathophysiology, falling under groups such as growth factors, anti-infective agents, 47 hormones, ion channel ligands, 48 and neurotransmitters. 49 They represent a unique class of pharmaceutical compounds, molecularly poised between small molecules and proteins, but with different biochemical and biological properties as compared to both.…”