In vitro antifungal activity of lasiodiplodin, isolated from endophytic fungus Lasiodiplodia pseudotheobromae J-10 associated with Sarcandra glabra and optimization of culture conditions for lasiodiplodin production

Abstract

A macrolide antibiotic, lasiodiplodin was isolated from the endophytic fungus (EF) Lasiodiplodia pseudotheobromae J-10 associated with the medicinal plant Sarcandra glabra. In vitro antifungal assay demonstrated the inhibitory activity of lasiodiplodin against the growth of eight phytopathogenic fungi, with the IC 50 values ranging between 15.50-249.10 µg/mL. The highest antifungal activities were recorded against Exserohilum turcicum, Colletotrichum capsici, and Pestalotiopsis theae, with IC 50 values of 15.50, 15.90, and 17.55 µg/mL, respectively. The underlying mechanism of the antifungal activity of lasiodiplodin against E. turcicum included the alteration of its colony morphology and disturbance of its cell membrane integrity. In addition, the optimization of L. pseudotheobromae J-10 culture conditions increased lasiodiplodin yield to 52.33 mg/L from 0.59 mg/L at pre-optimization. This is the rst report on the isolation and identi cation of antifungal compound from the EF L. pseudotheobromae J-10 associated with S. glabra, as well as on the optimization of L. pseudotheobromae J-10 culture conditions to increase lasiodiplodin yield. The results of this study support that lasiodiplodin is a natural compound with high potential bioactivity against phytopathogens, and provide a basis for further study of the EF associated with S. glabra.

In vitro antifungal effect of endophytic fungi of creole cucurbits of Yucatan

“…Compuestos antifúngicos secretados por L. pseudotheobromae inhibieron el crecimiento de R. solani, F. oxysporum y M. phaseolina, en 70, 65, 24% a los siete días, respectivamente (Sagaran y Sathiavelu 2023). Entre estos compuestos destaca la lasiodiplodina, causante de alteraciones en la morfología del micelio del fitopatógeno y la destrucción de su membrana celular (Luo et al 2023). Además de su efectividad en cuanto a competencia y micoparasitismo, la facilidad del aislamiento, el rápido crecimiento y la secreción de compuestos como la lasiodiplodina, hacen a L. pseudotheobromae una buena candidata para su uso en el biocontrol de una amplia gama de enfermedades en cultivos agrícolas.…”

“…Esto puede deberse a que las condiciones del cultivo de los hongos endófitos en el medio líquido no fueron las óptimas para la secreción de una concentración eficiente de compuestos antifúngicos. Por ejemplo, las mejores condiciones para la producción de lasiodiplodina son pH inicial de 7; temperatura de 28 ℃, volumen de líquido de 300/500 ml; relación C:N de 1:0 y tiempo de fermentación de 20 días (Luo et al 2023). No obstante, bajo las condiciones de cultivo y fermentación proporcionadas, F. equiseti demostró una mayor susceptibilidad contra los filtrados de los tres hongos endófitos seleccionados.…”

Synergistic Effects of Chemical Fungicides with Crude Extracts from Bacillus amyloliquefaciens to Control Northern Corn Leaf Blight

“…At present, active antimicrobial substances from other sources such as microbial metabolites have attracted more and more attention. Luo et al [25] isolated lasiodiplodin from the mycelium of Lasiodiplodia pseudotheobromae J-10, which had the strongest inhibitory effect on E. turcicum, with an EC 50 value of 15.50 µg•mL −1 . The mycelium treated with lasiodiplodin was severely distorted, nonseptate mycelia appeared, and the relative permeability of mycelium cells increased.…”