In Vitro Antifungal Evaluation of Seven Different Disinfectants on Acrylic Resins

Abstract

Objective. The aim of this study was to evaluate alternative methods for the disinfection of denture-based materials. Material and Methods. Two different denture-based materials were included in the study. Before microbial test, the surface roughness of the acrylic resins was evaluated. Then, the specimens were divided into 8 experimental groups (n = 10), according to microorganism considered and disinfection methods used. The specimens were contaminated in vitro by standardized suspensions of Candida albicans ATCC#90028 and Candida albicans oral isolate. The following test agents were tested: sodium hypochlorite (NaOCl 1%), microwave (MW) energy, ultraviolet (UV) light, mouthwash containing propolis (MCP), Corega Tabs, 50% and 100% white vinegar. After the disinfection procedure, the number of remaining microbial cells was evaluated in CFU/mL. Kruskal-Wallis, ANOVA, and Dunn's test were used for multiple comparisons. Mann Whitney U test was used to compare the surface roughness. Results. Statistically significant difference (P < 0.05) was found between autopolymerised and heat-cured acrylic resins. The autopolymerised acrylic resin surfaces were rougher than surfaces of heat-cured acrylic resin. The most effective disinfection method was 100% white vinegar for tested microorganisms and both acrylic resins. Conclusion. This study showed that white vinegar 100% was the most effective method for tested microorganisms. This agent is cost-effective and easy to access and thus may be appropriate for household use.

Investigation of Acrylic Resin Disinfection Using Chemicals and Ultrasound

“…C. albicans and S. oralis were selected to test the antimicrobial activity of the different treatment strategies in vitro, because C. albicans and streptococci are microorganisms commonly used in in vitro studies . On the other hand, in vivo studies demonstrate that the most common microorganisms normally found on the prosthetic materials are Staphylococcus aureus, C. albicans , and streptococci, Neisseria, micrococci, Pseudomonas , bacilli , and Acinetobacter species .…”

Comparison of Antimicrobial Activity between Chemical Disinfectants on Contaminated Orthodontic Pliers

“…2,7,11,[16][17][18] These microorganisms were effective in contaminating the pliers tested in this study, as showed in group 4. This group was designed with the aim of verifying the existence of pliers' contamination.…”

Effect of Denture Cleansers on Surface Roughness and Flexural Strength of Heat Cure Denture Base Resin-An In vitro Study

“…White vinegar is frequently used in concentration of 50% and 100% for disinfection of toothbrushes and denture base acrylic resins. Da Silva FC et al, Yildirim-Bicer AZ et al, advocated the use of 100% vinegar for 10 minutes as denture disinfectant especially against Candida Albicans [21,23].…”