In vitro antifungal potential of plant extracts against Fusarium oxysporum, Rhizoctonia solani and Macrophomina phaseolina.

Abstract

The antifungal activity of aqueous extracts of nine plants viz, Azadirachta indica, Parthenium hysterophorus, Momordica charantia, Allium sativum, Eucalyptus globules, Calotropis procera, Aloe vera, Beta vulgaris and Datura stramonium were assessed in vitro against Fusarium oxysporum f. sp. melongenae, Rhizoctonia solani and Macrophomina phaseolina, the soil borne phytopathogens. The assessment of fungitoxic effect was carried out by using three different concentrations i.e., 5, 10 and 20% against the test fungi, in terms of percentage of mycelial growth inhibition. The extract of A. sativum completely inhibited the mycelial growth of M. phaseolina at all the concentrations. The extracts of D. stramonium and E. globulus inhibited the mycelial growth of R. solani of 72%, and 70.7% respectively at 20% concentration, that of A. sativum, E. globulus and D. stramonium exhibited inhibition percentage of 63.3%, 61.8% and 61.1% respectively at 20% concentration on Fusarium oxysporum f. sp. melongenae. The application of plant extracts for disease management could be less expensive, easily available, non-polluting and eco-friendly.

The Extracts of Cinnamon and Clove as Potential Biofungicides against Strawberry Grey Mould

“…Petri dishes were incubated at 22 ± 2 • C in the dark for 7 days. The radial colony growth of the pathogen (plug excluded) was measured 2, 4 and 7 days after inoculation (DAI), and antifungal activity was expressed as the percentage inhibition of mycelial growth using the following formula [46]:…”

Phytochemical analysis and versatile in vitro evaluation of antimicrobial, cytotoxic and enzyme inhibition potential of different extracts of traditionally used Aquilegia pubiflora Wall. Ex Royle

“…The fungicidal behavior has also been documented by several authors in a large variety of taxa. The existence of antifungal compounds in higher plants is well known and considered useful for preventing plant diseases [ 62 ]. Five pathogenic fungal strains ( A. fumigatus, A. flavus, M. racemosus, F. solani and A. niger ) were tested against Aquilegia pubiflora leaf extracts using the well diffusion method at concentrations in the range of 0.5 mg/mL to 5 mg/mL.…”

“…Several authors have also observed fungicidal activity in a wide range of species. Antifungal chemicals are found in higher plants and are thought to be effective in avoiding plant infections [62]. Five pathogenic fungal strains (A. fumigatus, A. flavus, M. racemosus, F. solani and A. niger) were tested against Aquilegia pubiflora leaf extracts using the well diffusion method at concentrations in the range of 0.5 mg/ mL to 5 mg/mL.…”

Productivity and Post-Harvest Fungal Resistance of Hot Pepper as Affected by Potassium Silicate, Clove Extract Foliar Spray and Nitrogen Application

“…Among the fungal diseases affecting pepper, Alternaria alternata (Fries) Keissler is one of the most economically important; it is responsible for both pre- and postharvest crop losses, and consequently affects both yield and quality. The black mold infection can be developed during transport and storage of fresh pepper fruit [ 24 , 25 ]. The distinct fruit rot can cause substantial economic losses, since diseased fruits become unmarketable.…”