In vitro antileishmanial activity of leaf and stem extracts of seven Brazilian plant species

Paula, Renata Cristina de; Silva, Sydnei Magno da; Faria, Karen Ferraz; Frézard, Frédéric; Moreira, Carlos Otávio Fiúza; Foubert, Kenn; Lopes, Júlio César Dias; Campana, Priscilla Rodrigues Valadares; Rocha, Marina Pereira; Silva, Andréia Fonseca; Silva, Cláudia Gontijo; Pieters, Luc; Almeida, Vera Lúcia de

doi:10.1016/j.jep.2018.12.026

Cited by 12 publications

(4 citation statements)

References 45 publications

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“…The aim of this study was to identify antileishmanial compounds from the leaves of P. guajava and their putative mechanism of action. According to the inhibition percentage at 25 µg/mL, the ethanolic leaf extracts of P. guajava killed more than 50% of parasites, which is more than the reported antileishmanial activity of seven Brazilian plant species [25] and plants from the northwest of Morocco [26]. Moreover, the apolar fractions have interesting antileishmanial activity, with IC 50 values ranging from 1.62 ± 0.46 µg/mL to 2.89 ± 0.49 µg/mL and an SI measured on L. infantum amastigotes of about 26.…”

Section: Discussionmentioning

confidence: 80%

Antileishmanial Compounds Isolated from Psidium Guajava L. Using a Metabolomic Approach

et al. 2019

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With an estimated annual incidence of one million cases, leishmaniasis is one of the top five vector-borne diseases. Currently available medical treatments involve side effects, including toxicity, non-specific targeting, and resistance development. Thus, new antileishmanial chemical entities are of the utmost interest to fight against this disease. The aim of this study was to obtain potential antileishmanial natural products from Psidium guajava leaves using a metabolomic workflow. Several crude extracts from P. guajava leaves harvested from different locations in the Lao People's Democratic Republic (Lao PDR) were profiled by liquid chromatography coupled to high-resolution mass spectrometry, and subsequently evaluated for their antileishmanial activities. The putative active compounds were highlighted by multivariate correlation analysis between the antileishmanial response and chromatographic profiles of P. guajava mixtures. The results showed that the pooled apolar fractions from P. guajava were the most active (IC 50 = 1.96 ± 0.47 µg/mL). Multivariate data analysis of the apolar fractions highlighted a family of triterpenoid compounds, including jacoumaric acid (IC 50 = 1.318 ± 0.59 µg/mL) and corosolic acid (IC 50 = 1.01 ± 0.06 µg/mL). Our approach allowed the identification of antileishmanial compounds from the crude extracts in only a small number of steps and can be easily adapted for use in the discovery workflows of several other natural products.Molecules 2019, 24, 4536 2 of 13 of the human population to the parasite, and human behavior [3]. The disease is characterized by four forms; visceral leishmaniasis, post-kala-azar dermal leishmaniasis, cutaneous leishmaniasis, and mucocutaneous leishmaniasis [4]. Available medical treatments are not sufficient because of their extensive toxicity (e.g., the nephrotoxicity of amphotericin B, the teratogenicity of miltefosine), their lack of efficacy, their expensive cost (amphotericin B), the development of drug resistance (sodium stibogluconate and meglumine antimoniate), and non-specific targeting [4,5]. Fexinidazole, a 5-nitroimidazole, was recently in phase II of clinical trials on visceral leishmaniasis but exhibited a lack of efficacy, and in 2018 no new lead molecules were included in clinical trials [4].Control of leishmaniasis by traditional approaches (vector control and treatment of patients) is facing the difficulties of identifying new cost-effectively produced bioactive molecules able to be produced on an industrial scale, rapid development of drug resistance, and the lack of access to drugs by the threatened populations. These issues are at the heart of concerns of the WHO regarding the control of tropical diseases [6]. Therefore, combination therapies are used to improve the efficacy of antileishmanial therapies and can shorten treatment duration [7]. Furthering our fundamental knowledge through original approaches, herein concerning the activity of natural compounds from a promising plant species active against Leishmania spp., is vital to ov...

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Section: Discussionmentioning

confidence: 80%

Antileishmanial Compounds Isolated from Psidium Guajava L. Using a Metabolomic Approach

et al. 2019

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“…The correlation between Leishmania protease inhibition and anti-Leishmania activity was also investigated in leaf apolar extracts of Arrabidaea chica, Coccinia grandis and Aloe vera using hexane, ethyl acetate and ethanol extracts, respectively, a potato (Solanum tuberosum) tuber etanolic extract, and a Garcinia brasiliensis ethanolic pericarp extract. The correlation between PI and antileishmanial activity was only found in polar extracts containing polypeptides (de Paula et al 2019, Figueredo et al 2022. A serine protease polypeptide inhibitor (PTF3) from potato tubers also demonstrated strong antileishmanial activity against intracellular amastigotes of L. donovani and curative efficacy in infected hamsters (Paik et al 2020).…”

Section: Discussionmentioning

confidence: 98%

“…Promastigotes are employed in the initial investigation of leishmanicidal activity of natural products (Bekhit et al 2018, de Paula et al 2019). All C. spectabilis extracts were cytotoxic for L. amazonensis, and the leaf extracts were the most effective, especially CS-AE.…”

Section: Discussionmentioning

confidence: 99%

Antileishmanial effects of Crotalaria spectabilis Roth aqueous extracts on Leishmania amazonensis

PACHECO,

TEIXEIRA,

PASCHOAL

et al. 2023

An. Acad. Bras. Ciênc.

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Fifteen polar extracts from leaf, seed, pod, stem, flower and root of Crotalaria spectabilis were prepared using aqueous systems, based on the principles of green chemistry, and showed different protease inhibitor (PI) activities on trypsin, papain, pepsin and the extracellular L. amazonensis serine protease (LSPIII). The most pronounced inhibitory effect on LSPIII was observed in leaf (CS-P), root, stem, flower (CS-FPVPP) and pod (CS-VA) extracts. Crotalaria extracts exhibited low cytotoxicity on macrophages; however, they decreased the viability of L. amazonensis promastigotes and amastigotes, as observed in leaf (CS-AE, CS-P, CS-T and CS-PVPP), seed (CS-ST), flower and root (CS-RA) extracts. CS-P was chosen to study PI and secondary metabolites and a 10-12 kDa protein, analyzed by mass spectrometry, was identified as a serine PI homologous with papaya latex serine PI. Glycosylated flavonoids, such as quercetins, vitexin and tricin were the major secondary metabolites of CS-P. The presence of PIs in C. spectabilis is a new finding, especially in other organs than seeds since PIs have been reported only in seed legumes. Besides, this is the first report of antileishmanial activity of C. spectabilis extracts and the identification of serine polypeptide PI and glycosylated flavonoids from leaf.

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“…The sampling and analyses of native plants in the current work is authorized by Sistema Nacional de Gestão do Patrimônio Genético e do Conhecimento Tradicional Associado (SisGen), under license permit AD0077F [ 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 …”

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confidence: 99%

Essential Oils of Aromatic Plant Species from the Atlantic Rainforest Exhibit Extensive Chemical Diversity and Antimicrobial Activity

et al. 2022

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Microbial resistance, caused by the overuse or inadequate application of antibiotics, is a worldwide crisis, increasing the risk of treatment failure and healthcare costs. Plant essential oils (EOs) consist of hydrophobic metabolites with antimicrobial activity. The antimicrobial potential of the chemical diversity of plants from the Atlantic Rainforest remains scarcely characterized. In the current work, we determined the metabolite profile of the EOs from aromatic plants from nine locations and accessed their antimicrobial and biocidal activity by agar diffusion assays, minimum inhibitory concentration, time-kill and cell-component leakage assays. The pharmacokinetic properties of the EO compounds were investigated by in silico tools. More than a hundred metabolites were identified, mainly consisting of sesqui- and mono-terpenes. Individual plants and botanical families exhibited extensive chemical variations in their EO composition. Probabilistic models demonstrated that qualitative and quantitative differences contribute to chemical diversity, depending on the botanical family. The EOs exhibited antimicrobial biocidal activity against pathogenic bacteria, fungi and multiple predicted pharmacological targets. Our results demonstrate the antimicrobial potential of EOs from rainforest plants, indicate novel macromolecular targets, and contribute to highlighting the chemical diversity of native species.

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In vitro antileishmanial activity of leaf and stem extracts of seven Brazilian plant species

Cited by 12 publications

References 45 publications

Antileishmanial Compounds Isolated from Psidium Guajava L. Using a Metabolomic Approach

Antileishmanial Compounds Isolated from Psidium Guajava L. Using a Metabolomic Approach

Antileishmanial effects of Crotalaria spectabilis Roth aqueous extracts on Leishmania amazonensis

Essential Oils of Aromatic Plant Species from the Atlantic Rainforest Exhibit Extensive Chemical Diversity and Antimicrobial Activity

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