In Vitro Antimelanoma Properties of Verbena officinalis Fractions

Nisar, Rabia; Adhikary, Sanjay; Ahmad, Saeed; Alam, Mohammad A.

doi:10.3390/molecules27196329

Cited by 3 publications

(2 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…β-ionone identified in Sb-L-A ( Table 5 ) is also known to have significant antiproliferative, antimetastatic, and apoptosis-induction activities, both in vitro and in vivo [ 42 ]; however, its content was not high in Sb-L-A (0.89%). Lupulone isolated from hop ( Humulus lupulus L., Cannabaceae) [ 43 , 44 ] and the traditional medicinal plant Verbena officinalis [ 45 ] exhibits strong cytotoxic activity against several human cancer cell lines. Villosin is a naturally-occurring diterpenoid and abundant in Sb-L-A (25.48%) that also exhibits potent cytotoxic activities against several tumor cell lines [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata

Chen

Lin

et al. 2023

Plants

View full text Add to dashboard Cite

Sinningia bullata is a tuberous member of the flowering plant family Gesneriaceae. Prior to this work, the antibacterial, antioxidant, and cytotoxic properties of S. bullata were undetermined. Here, we prepared different extracts from the leaf, stem, and tuber of S. bullata and investigated their pharmacological activities. The leaf extract of S. bullata, obtained by 100% acetone (Sb-L-A), had the highest total flavonoid content, antioxidation capacity, and cytotoxic and antibacterial activities. Sb-L-A displayed a broad range of antibacterial activities against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The inhibition zones of Sb-L-A ranged from 8 to 30 mm and were in the following order: S. aureus > E. coli > P. aeruginosa. Incubation of B16F10 melanoma cells with Sb-L-A at a concentration of 80 μg/mL caused deaths at the rate of 96%, reduced migration by 100%, suppressed proliferation and colony formation by 99%, and induced apoptosis, which was observed in 96% of the B16F10 cells. In addition, the cytotoxic activities of Sb-L-A were synergistically enhanced when coacting with the antitumor drug epothilone B. Sb-L-A was also used to determine the cytotoxic effects against 4T1 mammary carcinoma cells. Sb-L-A of 60 μg/mL boosted the distribution of the G2 phase from 1.4% to 24.4% in the B16F10 cells. Accordingly, Sb-L-A might suppress melanoma cell proliferation by inducing G2 cell-cycle arrest. The most abundant compounds in Sb-L-A were identified using gas chromatography–mass spectrometry. The top two contents in this extract were adlupulone and villosin. Overall, the collective data in this study may indicate the pharmacological potentials of Sb-L-A for possible medical applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata

Chen

Lin

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…The protective efficacy against reactive oxygen species and free radicals is greater with natural antioxidants including polyphenols, carotenoids, and flavonoids due to their increased bioavailability. Chemical components that acted synergistically in the crude extract and allowed for its maximum activities were identified using spectroscopy of the separated ingredients [5].…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative Effects of Methanolic Root Extracts of Eichhornia crassipes Against a Skin Melanoma Cell Line: An In Vitro Study

Noufal¹,

Rajesh²,

Nair³

2023

Cureus

View full text Add to dashboard Cite

BackgroundMelanoma is the most aggressive form of skin cancer, accounting for 3% of all malignant cancers. Phytochemicals and their related compounds are found in various parts of the plant Eichhornia crassipes and have a variety of pharmacological actions. The current research was intended to compare and evaluate the anti-proliferative action of methanolic extracts of E. crassipes roots and petioles against the Sloan Kettering Melanoma (SK-Mel-5) cell line. Materials and methodsThe waters around Ezhikkara, Ernakulum, Kerala, were discovered to contain E. crassipes. We used a Soxhlet extractor to get this concentrated liquid. For this test, we employed a methanolic extract of roots and petioles to determine the extent to which different concentrations of the extract inhibited cell proliferation. Data on absorbance were reported as a mean standard deviation. Using Probit analysis, the IC 50 was calculated by evaluating the gradient of the regression line to get a value. ResultsConcentrations of methanolic root and petiole extracts of 12.5 µg/ml, 25 µg/ml, 50 µg/ml, 100 µg/ml, and 200 µg/ml were analyzed. The methanol petiole extract reduced the viability of SK-Mel-5 cells more than the root extract, with IC 50 values of 323.59 µg/ml and 174.70 µg/ml of the test sample concentration, respectively.The regression equation for the root extract was y = -0.1264x + 90.902 and R 2 = 0.845, and for the petiole extract, it was y = -0.2187x + 88.206 and R 2 = 0.917. ConclusionThe current study found that increasing the concentration of methanolic extracts of roots and petioles of E. crassipes exhibited an increased cell growth inhibition rate. However, methanolic petiole extracts were more cytotoxic than the roots. Thus, the current study demonstrated the therapeutic use of E. crassipes as an anticancer agent, thereby providing a valuable alternative for enabling the early management of melanoma.

show abstract

Vermicompost alleviates the growth, quality, photosynthetic and biochemical traits of Verbena officinalis under salt stress

Çiçek

Yücedağ

2023

Biologia

View full text Add to dashboard Cite

In Vitro Antimelanoma Properties of Verbena officinalis Fractions

Cited by 3 publications

References 45 publications

Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata

Cytotoxic, Antibacterial, and Antioxidant Activities of the Leaf Extract of Sinningia bullata

Antiproliferative Effects of Methanolic Root Extracts of Eichhornia crassipes Against a Skin Melanoma Cell Line: An In Vitro Study

Vermicompost alleviates the growth, quality, photosynthetic and biochemical traits of Verbena officinalis under salt stress

Contact Info

Product

Resources

About