In vitro antimicrobial activity of Achyranthes coynei Sant.

Ankad, Gireesh M.; Upadhya, Vinayak; Pai, Sandeep R.; Hegde, Harsha V.; Roy, Subarna

doi:10.1016/s2222-1808(13)60039-0

Cited by 9 publications

(10 citation statements)

References 10 publications

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“…Plant derived drugs are safer than the synthetic medicines, thus resulting in their wider utility. Emergence of multidrug resistant, extreme drug resistant and total drug resistant pathogens have left us with very few therapeutically effective antibiotics and has necessitated screening of medicinal plants for their antimicrobial activity [9,10].…”

Section: Introductionmentioning

confidence: 99%

Total Polyphenols, Antioxidant and Antimicrobial Activity of Leaves and Stem Bark Extracts of Allophylus cobbe (L.) Raeusch

Ankad

Upadhya

Pai

et al. 2014

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Allophylus cobbe is a small tree and an ethnomedicinally important plant species belonging to the family Sapindaceae, traditionally used to treat various ailments. Previous work on the species led to show ethanolic extracts to be active against Ranikhet disease virus and have showed effects on central nervous system and hypothermia. However, literature on its polyphenolic content, antioxidant and antimicrobial activity is inadequate. Because of its wide use in traditional medicine, it was decided to evaluate total polyphenols, antioxidant activity and to screen the antimicrobial activity of leaves and stem bark of A. cobbe. Leaves yielded 63.20 mg tannic acid equivalent per gram total phenolics and 17.39 mg quercetin equivalent per gram total flavonoids. A 0.01 % extract was sufficient to produce 32.78 % radical scavenging activity (RSA). 2,2 0 -Azinobis (3-ethyl benzo thiazoline-6-sulfonic acid) activity using ascorbic acid and Trolox Ò as standards were higher than ferric reducing antioxidant power activity. Both the extracts were more effective on gram positive organism, than gram negative organisms and were ineffective on fungal strains. The results herein report methanolic leaf and stem bark extracts of A. cobbe to possess good antibacterial and antioxidant activity.

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Section: Introductionmentioning

confidence: 99%

Total Polyphenols, Antioxidant and Antimicrobial Activity of Leaves and Stem Bark Extracts of Allophylus cobbe (L.) Raeusch

Ankad

Upadhya

Pai

et al. 2014

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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“…Two-fold tube dilution method was followed as explained by Ankad et al, 2013 to determine the Minimum Inhibitory Concentration (MIC) of leaf and root extracts against mentioned microorganisms.…”

Section: Tube Dilution Methodsmentioning

confidence: 99%

“…The potential of these plants as source for new drugs largely remain unexplored till date. As estimated out of 500,000 plant species, only a small percentage has been investigated for their biological or pharmacological activity (Ankad et al, 2013). Since olden days, humans have used herbs to treat infectious diseases and some of these traditional medicines are still included as a part of treatment in various ailments.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of leaf and root methanolic extracts from Vinca pusilla Murr.

Ankad¹,

Pednekar²,

Pai³

et al. 2016

Plant Sci. Today

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Vinca pusilla Murr. is a traditional medicinal plant used to treat several diseases. To substantiate the traditional medicinal utility of the plant, the present study aims at screening the antimicrobial activity of methanolic extracts of leaves and roots against five Gram positive, five Gram negative bacterial and four fungal strains. The minimum inhibitory concentration (MIC) were determined by two fold dilution assay. The results indicated that, leaf and root extracts were more effective on Bacillus subtilis and Staphylococcus aureus strains (MIC < 1 mg/mL). The tested organisms were sensitive to root extract compared to leaf extract. Fungal strains were resistant than the bacterial strains to both the extracts. Thus the present study illustrates the antimicrobial potential of the plant.

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“…The plant is used in the treatment of various ailments by traditional practitioners of the region [1,6]. The plant has been scientifically studied for its antimicrobial and antioxidant activities, correlating its medicinal properties with the phytoconstituents present [6][7][8][9][10][11].…”

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In Vitro Rapid Multiplication Protocol for Ex Situ Conservation of the Rare, Endemic Medicinal Plant Achyranthes coynei

et al. 2016

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!Achyranthes coynei is a rare, endemic species with potential medicinal properties. The present study aimed to develop an efficient in vitro rapid multiplication protocol for A. coynei. It was successfully propagated in vitro using apical shoot tips as explants. A combination of 6-benzylaminopurine (3.0 mg · L −1 ), thidiazuron (0.5 mg · L −1 ), and kinetin (0.5 mg · L −1 ) in MS basal proved best for multiple shoot induction, yielding 4.8 shoots per explant after 60 days of culture. The shoots were rooted with a frequency of 80% after 30 days of culture on MS agar medium fortified with IBA/NAA (0.5 mg · L −1 ) before being acclimatized in the greenhouse. The developed protocol will serve as a tool to restore the rare status of the plant and will append its sustainable utilization. Key wordsAchyranthes coynei · Amaranthaceae · in vitro · rapid multiplication · kinetin , 3-5]. The plant is used in the treatment of various ailments by traditional practitioners of the region [1,6]. The plant has been scientifically studied for its antimicrobial and antioxidant activities, correlating its medicinal properties with the phytoconstituents present [6][7][8][9][10][11].A. coynei is a perennial, profusely branching shrub growing uneven on road sides and on canal bunds along with Achyranthes aspera L. (l " Fig. 1 a) [1]. Fast erosion of medicinal plants has been reported from the study region [12,13]. Parameters such has deforestation, reduced rainfall, dam construction, grazing, fire wood collection, and expansion of cultivation land are hindering the natural habitat and population of medicinal plants including A. coynei [1, 12, 13]. The plant needs immediate attention to retain its medicinal properties displayed by the presence of richer triterpenoids over A. aspera [2,6,11]. The endemic and rare status of the plant by virtue of its assorted distribution makes it a vulnerable component of the community. And as quoted, endemic species confined to threatened ecosystems will certainly hit by extinction episodes and are most in need of rapid and effective conservation action [1,14]. Under these circumstances there is a need to facilitate an alternative strategy for its conservation and sustainable use. An in vitro system provides such an alternative tool. To the best of the authors knowledge, no work has been carried out on the in vitro multiplication of this rare, medicinal plant. Thus, the study was taken up to develop an efficient protocol for its rapid multiplication and ex situ conservation. Germination studies (1 to 30 day) showed 70% seed germination, and the first seed germinated on 7th day. The germination was higher in seeds deprived of a persistent calyx (80%) compared to seeds bearing a calyx (60 %).Higher numbers of multiple shoots were observed after 4 weeks of inoculation on BAP (3.0 mg · L −1 ), TDZ (0.5 mg · L −1 ), and Kn (1.5 mg · L −1 ) added individually to MS basal (l " Table 1). The shoot length was below 1 cm in BAP 3.0 mg · L −1 and TDZ 0.5 mg · L −1 (l " Fig. 1 b, c), whereas Kn 1.5 mg · L...

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In vitro antimicrobial activity of Achyranthes coynei Sant.

Cited by 9 publications

References 10 publications

Total Polyphenols, Antioxidant and Antimicrobial Activity of Leaves and Stem Bark Extracts of Allophylus cobbe (L.) Raeusch

Total Polyphenols, Antioxidant and Antimicrobial Activity of Leaves and Stem Bark Extracts of Allophylus cobbe (L.) Raeusch

Antimicrobial activity of leaf and root methanolic extracts from Vinca pusilla Murr.

In Vitro Rapid Multiplication Protocol for Ex Situ Conservation of the Rare, Endemic Medicinal Plant Achyranthes coynei

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