In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract

Abstract

This study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic crude extracts: hexane, dichloromethane, ethyl acetate and butanolic of Alpinia zerumbet and A. purpurata by the agar drop diffusion method in order to screen the main compounds involved in antimicrobial activity. Leaves of A. zerumbet (Pers.) B.L. Burtt et R.M. Sm. and A. purpurata (Vieill) K. Schum adult plants were collected and then dried and macerated in 70% ethanol. Leaf extracts were further partitioned using solvents in increasing polarity. Dichloromethane fractions were analyzed by gas chromatography/mass spectrometry (GC/MS). The major compound in the dichloromethane fraction of both species was the hexadecanoic acid (palmitic acid). By drop diffusion assay, the antimicrobial activity of crude extract, as well as fractions of hexane, dichloromethane, ethyl acetate and butanolic, was evaluated against pathogenic bacteria and fungi. No bacteria were inhibited. However, the dichloromethane fraction exhibited promising antifungal activity against the following fungi tested: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis and M. gypseum.

Phytogenics in Ginger, Origanum vulgare, and Syzygium aromaticum and Their Potential as a Feed Additive against Clostridium perfringens in Broiler Production

“…Alpinia spp. belongs to the ginger family, Zingiberaceae, and its extracts have been found to contain active compounds for therapeutic properties [99], including antioxidant, anti-inflammatory, and anti-microbial activities (Table 3) [100][101][102][103][104][105][106][107][108][109][110][111][112][113]. Effective against food contaminants that cause hepatotoxicity; possesses anti-inflammatory properties [112,113] 5,6-dehydrokawain (DK) and dihydro-5,6-dehydrokawain (DDK), found in the rhizomes and leaves of Alpinia zerumbet, exhibit antioxidant [101] characteristics and pro-foundly inhibit collagenase, elastase, hyaluronidase, and tyrosinase activity [100].…”

“…The ethanolic extracts from the dried seeds of A. katsumadai have anti-adhesive effects against Campylobacter jejuni [109], while methanolic extracts from flowers and rhizomes are effective against Micrococcus luteus and have been used to treat intestinal infections and other diseases [110]. The palmitic acid present in the leaf extracts of Alpinia zerumbet and Alpinia pupurpurata also have anti-fungal properties against Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis, and M. gypseum [111]. Alpinia zerumbet leaves and rhizomes can be hydro-distilled to produce zerumbone [112], which has been proven to possess anti-inflammatory and protective effects against dietary contaminants that cause hepatotoxicity [113].…”

Technological mapping of plant-based patents for treating bovine mastitis

“…Plants are known to have a composition of bioactive metabolites, which have antimicrobial, antiin ammatory, and healing properties (Simoni, 2011;BrandeLLI, 2017;Freitas et al, 2019;Sera m et al, 2020;Victorio et al, 2021). In addition to their antimicrobial activity, the anti-in ammatory and healing properties of plants also contribute to the treatment of bovine mastitis, and because of this, they are used in the composition of medicinal products (Avancini et al, 2008;Vidal et al, 2023).…”