2011
DOI: 10.5897/ajmr10.692
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In vitro antimicrobial activity of leaf extract of Berlina grandiflora Hutch. and Dalz.

Abstract: Antimicrobial properties of Berlina grandiflora Hutch. and Dalz. leaf extract were investigated against both clinical and laboratory isolates. Susceptibility of these isolates to the extract was determined using disc diffusion method. The antimicrobial screening had wide range of activity on Escherichia coli, Stapylococcus aureus and Streptococcus spp. The diameter of zone of inhibition by the extract was 7, 8.7 and 9 mm respectively. The minimum inhibitory concentrations (MIC) were 32.81, 19.38 and 11.72 µg/m… Show more

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Cited by 5 publications
(3 citation statements)
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“…The antimicrobial activity of the ethanol extract was assayed using disc agar diffusion method of Akuodor et al (10). Before the assay, purity plates of required microbial isolates were isolated and biochemical tests carried out to confirm the identity of the organisms.…”
Section: Antimicrobial Assaymentioning
confidence: 99%
“…The antimicrobial activity of the ethanol extract was assayed using disc agar diffusion method of Akuodor et al (10). Before the assay, purity plates of required microbial isolates were isolated and biochemical tests carried out to confirm the identity of the organisms.…”
Section: Antimicrobial Assaymentioning
confidence: 99%
“…No inhibitory effect was observed for P. notatum and R. stolonifer. The minimum inhibitory concentration (MIC) ( Table 3) varied with organism: 25 mg/ml for S. aureus and E. coli, 50 mg/ml for B. substilis, S. typhi and C. albicans and 100 mg/ml for P. aeruginosa, K. pneumoniae and A. niger and are comparable to essential oils of other origins [28,41,42]. These results indicate that the essential oil of the plant M. alternifolius possesses antibacterial activity.…”
Section: Minimum Inhibitory Concentration (Mic) Of Essential Oil Of Mmentioning
confidence: 77%
“…Discrete colonies of fresh cultures from different bacterial isolates were carefully mixed in 5 ml nutrient broth and incubated for 24 h at 37 °C prior to antimicrobial susceptibility testing. The antimicrobial activity of the ethanol leaf extract of S. lehmbachii was assayed using agar well diffusion method of Kumar et al ( 2012 ) and Akuodor et al ( 2011 ). Broth cultures, 0.5 ml, of the isolate containing 10 5 cfu/ml of organism were properly introduced into sterile petri-dish and 15 ml of Muller Hinton agar was added.…”
Section: Methodsmentioning
confidence: 99%