In Vitro Antimicrobial Activity of an Experimental Dentifrice Based on Ricinus Communis

Abstract

This study evaluated the antimicrobial activity of a Ricinus communis-based experimental dentifrice for denture hygiene against the following standard strains: Staphylococcus aureus, Escherichia coli, Streptococcus mutans, Enterococcus faecalis, Candida albicans and Candida glabrata. The minimum inhibitory concentration (MIC) assay was performed with R. communis in pure oil at 2.5%. Only E. coli was not inhibited by R. communis, but the MIC (0.0781%) was effective against the other microorganisms. From these results it was determined the R. communis concentrations for experimental dentifrices, 1, 2, 5 and 10%, which were evaluated by the test-well diffusion in agar. The commercial dentifrices Colgate, Trihydral and Corega Brite were tested for comparative purposes. The diameter of the zones of bacterial growth inhibition produced around the wells was measured (in mm) with a rule under reflected light. Data were analyzed statistically by analysis of variance and Tukey's post-hoc test (α=0.05). Neither the commercial nor the experimental dentifrices were effective against E. coli. The experimental dentifrices containing R. communis at 2, 5 and 10% presented action against S. mutans, S. aureaus and E. faecallis. The experimental dentifrices showed no antimicrobial activity against Candida spp. and E. coli in any of the tested concentrations. Trihydral was the most effective. Comparing the experimental dentifrices, the product with 10% R. communis produced the largest zones of bacterial growth inhibition and had similar antimicrobial activity to the commercial dentifrices, except against S. aureus.

Clinical trial for evaluation of Ricinus communis and sodium hypochlorite as denture cleanser

“…In addition, it is also biocompatible 9 and has detergent properties. There are studies evaluating the efficiency of R. communis solution in achieving complete denture hygiene, although experimental designs are diverse and results are inconclusive 2 , 10 , 19 , 20 , 22 , 23 .…”

“…Since most of the current methods used for denture hygiene do not present all these characteristics, numerous studies have been conducted in an attempt to find an optimal protocol 2 , 4 , 7 , 10 , 16 , 19 , 20 , 22 - 24 , 26 , 27 , 29 .…”

“…It may be subjected to various chemical processes to obtain by-products used in the pharmaceutical and cosmetic industry, in the production of lubricants, polymers, biodiesel 11 , 12 , and more specifically in medical and dental products. Although a few studies have focused on the use of R. communis in complete dentures, the available results are promising 2 , 4 , 19 , 20 , 22 , 23 , particularly at a concentration of 10% 10 , 22 , 23 . However, although controlled clinical trials assess this oil’s ability to remove biofilm, its antimicrobial properties and patient acceptability are inconclusive, and call for further investigation.…”

In Vitro Evaluation of Resilient Liner after Brushing with Conventional and Experimental Ricinus communis‐Based Dentifrices

“…Studies have shown that the prevalence of different Candida species is significantly higher in denture wearers and that Candida albicans is frequently implicated in denture stomatitis (DS), inflammation of the mucosa underlying a complete denture . Denture cleansers containing Ricinus communis are in development with different formulations and concentrations . In a preliminary study, Leite et al evaluated the antimicrobial activity of an R. communis ‐based experimental dentifrice for denture hygiene and demonstrated that the R. communis dentifrice at 10% caused the largest zones of bacterial growth inhibition and that it had similar antimicrobial activity to the commercial dentifrices, except against S. aureus .…”

“…15 Denture cleansers containing Ricinus communis are in development with different formulations and concentrations. 6,[16][17][18][19] In a preliminary study, Leite et al 17 evaluated the antimicrobial activity of an R. communis-based experimental dentifrice for denture hygiene and demonstrated that the R. communis dentifrice at 10% caused the largest zones of bacterial growth inhibition and that it had similar antimicrobial activity to the commercial dentifrices, except against S. aureus. Roselino et al 20 concluded that the same formulation of the experimental R. communis dentifrice did not cause surface roughness and color alteration in artificial teeth.…”