In vitro Antimicrobial Effects of Silver Nanoparticles on Microorganisms Isolated from Dog with Otitis External

Bae, Seulgi; Yun, Sung-Ho; Oh, Tae-Ho

doi:10.17555/jvc.2017.12.34.6.425

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“…Many products containing AgNPs are now used in human medical care. Some products intended for dogs that contain AgNPs are also available, but little is known about their microbial activity against veterinary relevant pathogens (Bae et al., 2017). A single preliminary study of the antibiofilm activity of AgNPs on S. pseudintermedius isolated from dogs currently exists (Meroni et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm activity of silver nanoparticles against biofilm forming Staphylococcus pseudintermedius isolated from dogs with otitis externa

Seo

Bae

2021

Veterinary Medicine & Sci

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Background Silver nanoparticles (AgNPs) are known to possess antimicrobial properties. Although the antibiofilm activity of AgNPs has been demonstrated in humans, this activity has not yet been elucidated in veterinary medicine. Objectives The purpose of this study was to evaluate the antibiofilm activity of silver nanoparticles against Staphylococcus pseudintermedius. Methods Ten isolates of S. pseudintermedius obtained from dogs with otitis externa were treated with AgNPs, and the antibiofilm activity was measured using a modified microtiter plate and Congo red agar (CRA) method and scanning electron microscopy. Results AgNPs displayed a significant dose‐dependent antibiofilm activity and reduced biofilm formation at concentrations of 20 and 10 µg/ml (p < 0.05). S. pseudintermedius exposed to 20 µg/ml of AgNPs formed less bacterial slime compared to the controls on CRA plates. Scanning electron micrographs showed that the biofilm had few individually scattered cells along its surface when treated with AgNP concentrations of 20 and 10 µg/ml. Untreated surfaces showed an aggregated biofilm. Conclusions Our results suggested that AgNP may be a valuable alternative antibiofilm agent for canine otitis externa.

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Section: Introductionmentioning

confidence: 99%