In vitro Antimicrobial Properties of Pluronic F-127 Injectable Thermoresponsive Hydrogel

Abstract

Pluronic F-127 (PF-127) hydrogel is a versatile biomaterial with promising applications in drug delivery, tissue engineering, and regenerative medicine. PF-127 has antiadhesive activity that prevents bacterial adhesion by creating a hydrated layer on the bacterial surface. This property makes PF-127 suitable for preventing implant-associated infections. In this study, we aimed to evaluate the antibacterial properties of PF-127 using field isolates of Staphylococcus aureus (Gram-positive bacteria) and Escherichia coli (Gram-negative bacteria) and compare them with different antibiotic standards. The antimicrobial potential was assessed using disk diffusion assays with four standard concentrations (20%, 25%, 30%, and 40%). The test microorganisms were inoculated on agar plates, and sterile filter paper disks infused with PF-127 hydrogels were placed alongside standard antibiotic disks. After incubation, the inhibition zones were measured to determine antimicrobial activity. Our results showed that PF-127 lacked intrinsic antimicrobial activity against S. aureus and E. coli at the tested concentrations. In conclusion, PF-127 hydrogel is a promising neutral carrier hydrogel system for loading antibiotics and antimicrobial compounds. Its unique properties, such as biocompatibility and thermo-responsive behaviour, combined with its antiadhesive activity, make it an ideal candidate for various biomedical applications.

Pluronic F-127 Hydrogel for Delivering Antimicrobial Agents: A Bibliometric Analysis using Scopus Database

“…The unique properties of PF-127, including its thermosensitivity, biocompatibility, and ability to sol-gel transition, make it an attractive choice for controlled and sustained release of antimicrobial agents. 6,7 This hydrogel system offers several advantages, such as localized drug delivery, prolonged drug release, and the ability to create an optimal environment for wound healing while combating infections. 8 PF-127 hydrogel lacks intrinsic antimicrobial activity against Staphylococcus aureus and Escherichia coli, but its antiadhesive properties and biocompatibility make it a promising neutral carrier system for antibiotics and antimicrobial compounds in biomedical applications.…”

“…8 PF-127 hydrogel lacks intrinsic antimicrobial activity against Staphylococcus aureus and Escherichia coli, but its antiadhesive properties and biocompatibility make it a promising neutral carrier system for antibiotics and antimicrobial compounds in biomedical applications. 7 Its antiadhesive properties indirectly contribute to preventing microbial infections by inhibiting bacterial adhesion. PF-127 hydrogel reduces the likelihood of bacterial colonization and subsequent infection.…”