In Vitro Antimicrobial Susceptibility of<i>Mycobacterium massiliense</i>Recovered from Wound Samples of Patients Submitted to Arthroscopic and Laparoscopic Surgeries

Cardoso, Alessandra Marques; Junqueira-Kipnis, Ana Paula; Kipnis, André

doi:10.1155/2011/724635

Cited by 13 publications

(9 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…bolletii , which was characterized by a specific rpoB sequevar (accession number EU117207) and two closely related pulsed field gel electrophoresis (PFGE) patterns, was responsible for these infections [4]. This strain was susceptible to amikacin and clarithromycin and resistant to tobramycin, ciprofloxacin, sulfamethoxazole, minocycline and doxycycline [6], [7], [8]. The strain was highly resistant to glutaraldehyde and susceptible to orthophthaldehyde and peracetic acid-based solutions [3], [9].…”

Section: Introductionmentioning

confidence: 99%

The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1β Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp. bolletii

et al. 2013

View full text Add to dashboard Cite

BackgroundAn extended outbreak of mycobacterial surgical infections occurred in Brazil during 2004–2008. Most infections were caused by a single strain of Mycobacterium abscessus subsp. bolletii, which was characterized by a specific rpoB sequevar and two highly similar pulsed-field gel electrophoresis (PFGE) patterns differentiated by the presence of a ∼50 kb band. The nature of this band was investigated.Methodology/Principal FindingsGenomic sequencing of the prototype outbreak isolate INCQS 00594 using the SOLiD platform demonstrated the presence of a 56,264-bp circular plasmid, designated pMAB01. Identity matrices, genetic distances and phylogeny analyses indicated that pMAB01 belongs to the broad-host-range plasmid subgroup IncP-1β and is highly related to BRA100, pJP4, pAKD33 and pB10. The presence of pMAB01-derived sequences in 41 M. abscessus subsp. bolletii isolates was evaluated using PCR, PFGE and Southern blot hybridization. Sixteen of the 41 isolates showed the presence of the plasmid. The plasmid was visualized as a ∼50-kb band using PFGE and Southern blot hybridization in 12 isolates. The remaining 25 isolates did not exhibit any evidence of this plasmid. The plasmid was successfully transferred to Escherichia coli by conjugation and transformation. Lateral transfer of pMAB01 to the high efficient plasmid transformation strain Mycobacterium smegmatis mc2155 could not be demonstrated.Conclusions/SignificanceThe occurrence of a broad-host-range IncP-1β plasmid in mycobacteria is reported for the first time. Thus, genetic exchange could result in the emergence of specific strains that might be better adapted to cause human disease.

show abstract

Section: Introductionmentioning

confidence: 99%

The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1β Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp. bolletii

et al. 2013

View full text Add to dashboard Cite

show abstract

“…abscessus subsp. massiliense isolates GO 01, GO 06, GO 07, GO 08, GO 13, and GO 18, were randomly chosen from a collection bank of isolates from a Brazilian epidemic outbreak that occurred in Goiânia, State of Goiás during 2005 and 2007 [ 5 ], and CRM0020 ( M . abscessus subsp.…”

Section: Methodsmentioning

confidence: 99%

“…massiliense infections is to use a combination of drugs to avoid the development of resistance. Currently, the most commonly used chemotherapy includes the use of clarithromycin (CLR) together with amikacin (AMK) [ 5 ], moxifloxacin (MXF) or cefoxitin (FOX) [ 6 ]. The major problem of chemotherapy, in addition to intrinsic resistance, is the development of resistant strains that vary from 4.5% resistance to CLR to 18% to FOX [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha

et al. 2016

Self Cite

View full text Add to dashboard Cite

Mycobacterium abscessus subsp. massiliense, a rapidly growing mycobacteria (RGM) that is becoming increasingly important among human infectious diseases, is virulent and pathogenic and presents intrinsic resistance to several antimicrobial drugs that might hamper their elimination. Therefore, the identification of new drugs to improve the current treatment or lower the risk of inducing resistance is urgently needed. Wasp venom primarily comprises peptides that are responsible for most of the biological activities in this poison. Here, a novel peptide Polydim-I, from Polybia dimorpha Neotropical wasp, was explored as an antimycobacterial agent. Polydim-I provoked cell wall disruption and exhibited non-cytotoxicity towards mammalian cells. Polydim-I treatment of macrophages infected with different M. abscessus subsp. massiliense strains reduced 40 to 50% of the bacterial load. Additionally, the Polydim-I treatment of highly susceptible mice intravenously infected with M. abscessus subsp. massiliense induced 0.8 to 1 log reduction of the bacterial load in the lungs, spleen, and liver. In conclusion, this is the first study to show the therapeutic potential of a peptide derived from wasp venom in treating mycobacteria infections. Polydim-I acts on the M. abscessus subsp. massiliense cell wall and reduce 40–90% of the bacterial load both in vitro and in vivo. The presented results encourage further studies on the use of Polydim-I as one of the components for M. abscessus subsp. massiliense treatment.

show abstract

“…bolletii strains. In addition, previous publications have confirmed that outbreak strains, whether harboring the pMAB01 plasmid or not, are fully susceptible to amikacin (AMI) (MIC ϭ 4 g/ml or 8 g/ml) (8)(9)(10).…”

mentioning

confidence: 93%

Demonstration of Plasmid-Mediated Drug Resistance in Mycobacterium abscessus

et al. 2014

View full text Add to dashboard Cite

b Plasmid-mediated kanamycin resistance was detected in a strain of Mycobacterium abscessus subsp. bolletii responsible for a nationwide epidemic of surgical infections in Brazil. The plasmid did not influence susceptibility to tobramycin, streptomycin, trimethoprim-sulfamethoxazole, clarithromycin, or ciprofloxacin. Plasmid-mediated drug resistance has not been described so far in mycobacteria. P lasmid-mediated drug resistance is uncommon in mycobacteria. A 56,267-bp circular plasmid of the broad-host-range IncP-1␤ subgroup was detected in a strain of Mycobacterium abscessus subsp. bolletii (formerly known as Mycobacterium massiliense [1] and Mycobacterium abscessus subsp. massiliense [2]) that was responsible for a nationwide outbreak of mycobacterial surgical infections in Brazil in 2004Brazil in to 2008. This plasmid, named pMAB01, contains a complete system for conjugative DNA transfer and two genetic load regions carrying antimicrobial resistance genes, including the Tn5393c streptomycin resistance (str) transposon and a typical class 1 integron with an incorporated gene cassette encoding an aminoglycoside 6=-N-acetyltransferase (aac[6=]-Ib), the dihydropteroate synthase type 1 gene (sul1), and a conserved open reading frame (orf5) that carries a GCN5-like N-acetyltransferase (3). We investigated the contribution of this plasmid to antimicrobial drug resistance in M. abscessus and in Escherichia coli.The MICs of streptomycin (STR), kanamycin (KAN), tobramycin (TOB), trimethoprim-sulfamethoxazole (SXT), clarithromycin (CLR), and ciprofloxacin (CIP) were evaluated against the prototype M. abscessus subsp. bolletii outbreak strain INCQS 00594, the INCQS 00594 strain cured of the plasmid after 10 in vitro passages in liquid medium, and two additional outbreak isolates, one harboring the pMAB01 plasmid and one lacking it (Fig. 1A). Two E. coli strains, BL21(DE3) and a spontaneous mutant of E. coli C600 (5) showing nalidixic acid resistance (C600Nal r ), and the same strains harboring the pMAB01 plasmid were tested (Fig. 1B). The experiments performed to introduce pMAB01 into E. coli were detailed in a previous publication (3). In brief, competent E. coli BL21(DE3) isolates were transformed by electroporation with the pMAB01 plasmid purified from the INCQS 00594 isolate. In addition, mating experiments were carried out to confirm the conjugative capacity of the pMAB01 plasmid. Conjugative transfer of pMAB01 from M. abscessus to E. coli BL21(DE3) occurred at a frequency of 5.24 ϫ 10 Ϫ8 transconjugants/recipient and from the E. coli BL21(DE3) transconjugant to another E. coli strain, C600Nal r , at a frequency of 1.14 ϫ 10 Ϫ6 transconjugants/recipient (3). The E. coli C600Nal r strain was used as recipient in mating experiments with two E. coli strains to simplify the screening of E. coli transconjugants on nalidixic acid plates.The MICs were determined by the broth microdilution method according to Clinical and Laboratory Standards Institute documents M24-A2 and M100-S23 (6, 7). M. abscessus subsp. bolletii ...

show abstract

In Vitro Antimicrobial Susceptibility ofMycobacterium massilienseRecovered from Wound Samples of Patients Submitted to Arthroscopic and Laparoscopic Surgeries

Cited by 13 publications

References 22 publications

The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1β Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp. bolletii

The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1β Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp. bolletii

Antimycobacterial Activity of a New Peptide Polydim-I Isolated from Neotropical Social Wasp Polybia dimorpha

Demonstration of Plasmid-Mediated Drug Resistance in Mycobacterium abscessus

Contact Info

Product

Resources

About