In vitro antioxidant activities of root extract of Asparagus racemosus Linn.

Abstract

ObjectiveThe purpose of the study is to investigate potential of antioxidant property of ethanolic root extract of Asparagus racemosus Linn (EEAR).MethodsIn vitro evaluation antioxidant property of EEAR was done using various methods like DPPH scavenging activity, hydroxyl radical scavenging activity, and nitric oxide scavenging activity. HPTLC fingerprint analysis was performed for qualitative determination of possible number of components from the ethanolic extract. Acute toxicity study was performed in Wistar rat and an OECD guideline 423 was followed.ResultsThe yield value was found 0.96% from EEAR. A concentration of 468.57 ± 3.002 μg/ml of probable antioxidant material from EEAR was required to scavenge 50% of DPPH. The IC50 value of EEAR were found to be 508.17 ± 7.37 μg and 416.57 ± 5.08 μg when determined by hydroxyl radical and nitric oxide scavenging assay respectively. The reducing powers of EEAR was 0.295 ± 0.0037 at 125 μg/ml and increased to 0.934 ± 0.0005 at 500 μg/ml. HPTLC fingerprint data supports several basic informations like isolation, purification, quality evaluation and standardization. No sign of toxicity was observed after treated with 2000 mg/kg of EEAR.ConclusionThe obtained data highlight the potential role of EEAR as a source of natural antioxidants.

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“…According to this study, it may be assumed that DPPH gets reduced to its resultant hydrazine form by EEAN. This reduction clues to the color conversion of DPPH from purple to yellow based on a number of electrons taken up [12,[34][35].…”

“…Mother Nature provides different ailments to prevent or cure various diseases and among them, several plants possess numbers of medicinal properties [9][10][11][12]. Traditional healers use their knowledge to cure or treat different diseases with the help of medicinal plants [13][14][15].…”

Analysis of alkaloids (indole alkaloids, isoquinoline alkaloids, tropane alkaloids)

“…Plant secondary metabolites are a diverse range of biologically active molecules having multiple pharmacological actions like antimicrobial, stimulant, analgesic, anthelmintic, anticoagulant, antiacne, and antioxidant, among others (Debnath et al, 2012;Bhakta, 2012, 2013;Dey et al, 2012bDey et al, , 2014Karuna et al, 2018aKaruna et al, , 2018bKundu et al, 2019). Different synthetic compounds show potential biological activity in vitro as well as in vivo ( Jeon et al, 2017;Jeong et al, 2017a, b;Kim et al, 2018;Lee et al, 2018Lee et al, , 2019Richa et al, 2019;Tae et al, 2018), and various formulations of existing drugs are also developed to increase the safety profile (Gorain et al, 2014;Karmakar et al, 2015;Maity et al, 2016).…”

Therapeutic value of steroidal alkaloids in cancer: Current trends and future perspectives

“…According to the World Health Organization (WHO), approximately 80% of the global population (representing approximately four billion people) living in developing countries depend on plant‐based traditional medicines for their primary health care . Ethnomedicinal properties of traditional medicines, along with their diverse medical applications in various diseases, have been well‐characterized in several publications . Goat bitter apple or soda apple ( Solanum aculeastrum ), thorn apple ( Solanum incanum L.), tomato ( Lycopersicon esculentum M.), false daisy ( Eclipta alba L.) and Christmas box ( Sarcococca saligna ) are well‐known plants possessing anticancer activity.…”

“…18,19 Ethnomedicinal properties of traditional medicines, along with their diverse medical applications in various diseases, have been well-characterized in several publications. [20][21][22][23][24][25][26] Goat bitter apple or soda apple (Solanum aculeastrum), thorn apple (Solanum incanum L.), tomato (Lycopersicon esculentum M.), false daisy (Eclipta alba L.) and Christmas box (Sarcococca saligna) are well-known plants possessing anticancer activity. Solamargine, tomatidine, solasonine, αsolanine and α-chaconine are natural products isolated from terrestrial plants and exhibit potent anticancer activity (Supporting Information S1, .…”