In Vitro Antioxidant Activity and Hepatoprotective Effects of Lentinula edodes against Paracetamol-Induced Hepatotoxicity

Abstract

Background: The objective of this study was to investigate the antioxidant and hepatoprotective effects of methanolic extracts of L. edodes and the determination of their total phenolics content. Results: The amount of total phenolics was estimated to be 70.83 mg Gallic Acid Equivalent (GAE) per gram of dry extract. The antioxidant activity of the L. edodes extract was 39.0% at a concentration of 1 mg/mL and was also concentration dependant, with an EC50 value of 4.4 mg/mL. Different groups of animals (Wister albino mice) were administered paracetamol (1 g/kg, p.o.). L. edodes extract at a dose of 200 mg/kg was administered to the paracetamol treated mice for seven days. The effects of L. edodes extract on serum transaminases (SGOT, SGPT), alkaline phosphatase (ALP) and bilirubin were measured in the paracetamol-induced hepatotoxic mice. L. edodes extract produced significant (p < 0.05) hepatoprotective effects by decreasing the activity of serum enzymes and bilirubin. Conclusion: From these results, it was suggested that L. edodes extract could perhaps protect liver cells from paracetamol-induced liver damage by its antioxidative effect on hepatocytes, hence diminishing or eliminating the harmful effects of toxic metabolites of paracetamol.

Radical Scavenging Capacity of Seeds and Leaves Ethanol Extracts ofCynara Scolymus L.—A Comparative Study

“…Results obtained by the spectrophotometry method are presented on Fig. 3 (1,21). As, the value of this parameter is lower as the extract antioxidant activity is higher (14).…”

Antioxidant activity of methanol extracts of different parts of Lantana camara

“…The total phenolic content of the extracts were d e t e r m i n e d a c c o r d i n g t o t h e F o l i n -C i o c a l t e u spectrophotometric method [11] with some modifications. To prepare a calibration curve, 0, 1, 2, 3, 5 and 10 mL of the phenol (gallic acid) stock solution (5 mg/mL) was added into 100 mL volumetric flasks, and then diluted to volume with water.…”

Hepatoprotective Effects of Mushrooms

“…Nevertheless, chemical toxins (including acetaminophen, carbon tetrachloride, thioacetamide, ethanol, D-galactosamine, diethylnitrosamine and dimethylnitrosamine) are often used as the model substances causing experimental hepatocyte injury under both in vivo and in vitro conditions [10,[34][35][36][37][38][39][40][41][42][43][44][45][46][47].…”