In vitro antioxidant activity of defatted seed extracts of Ocimum sanctum on rat PC-12 cells and its inhibitory efficacy with receptors of oral squamous cell carcinoma

Sharma, Yash; Bharadwaj, Mausumi; Srivastava, Nidhi; Kaur, Amritpal; Kumar, Manish; Agarwal, Mohini; Bahl, Yash; Bala, Kumud

doi:10.1016/j.indcrop.2020.112668

Cited by 16 publications

(10 citation statements)

References 22 publications

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“…Similarly, in HeLa cells, APA (Sox) extract showed the highest SOD, GST activity, GSH content, and the least MDA content, whereas APA (Mac) extract showed the highest catalase activity. Previous studies on extracts of Ocimum sanctum defatted seeds possessing polyphenolic flavonoids demonstrated its antioxidant enzyme activity in-vitro on Rat PC-12 cells 44 . However, no previous research on the antioxidant enzyme activity of A. precatorius seed extracts on cervical cancer cells has been done, to our knowledge.…”

Section: Discussionmentioning

confidence: 92%

“…Absorbance of the mixture was measured at 725 nm. Final results were represented as quercetin equivalents (mg QE)/g of extract and tannic acid equivalents (mg TA)/g of extract, respectively 44 , 49 , 51 . All the concentrations were calculated using a standard calibration plot.…”

Section: Methodsmentioning

confidence: 99%

“…The cells were harvested by washing with PBS followed by trypsinization. Further, the samples were centrifuged at 10,000 rpm for 20 min at 4 °C to obtain the cell lysate which was further used for estimation of enzyme and non-enzyme content in accordance with standardized procedures 44 .…”

Section: Methodsmentioning

confidence: 99%

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In-vitro antiproliferative efficacy of Abrus precatorius seed extracts on cervical carcinoma

Kaur

Sharma

Kumar³

et al. 2022

Sci Rep

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Abrus precatorius is a tropical medicinal plant with multiple medicinal benefits whose seeds have not yet been studied against cervical cancer. Herein, we have assessed the antioxidant and antiproliferative properties of seed extracts (ethyl acetate and 70% ethanol) prepared from Soxhlet and Maceration extraction methods against Hep2C and HeLa Cells. We observed that the APE (Sox) extract had a significantly higher total flavonoid content, APA (Mac) extract had a high total phenolic content, and APA (Sox) extract had a high total tannin content. Further, HPLC analysis of extracts revealed the presence of tannic acid and rutin. Moreover, APA (Sox) exhibited the highest free radical scavenging activity. APE (Mac) had the best antiproliferative activity against Hep2C cells, while APA (Sox) had the best antiproliferative activity against HeLa cells. In Hep2C cells, APE (Mac) extract revealed the highest SOD, catalase activity, GSH content, and the lowest MDA content, whereas APA (Mac) extract demonstrated the highest GST activity. In HeLa cells, APA (Sox) extract showed the highest SOD, GST activity, GSH content, and the least MDA content, whereas APA (Mac) extract showed the highest catalase activity. Lastly, docking results suggested maximum binding affinity of tannic acid with HER2 and GCR receptors. This study provides evidence that A. precatorius seed extracts possess promising bioactive compounds with probable anticancer and antioxidant properties against cervical cancer for restricting tumor growth.

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Section: Discussionmentioning

confidence: 92%

Section: Methodsmentioning

confidence: 99%

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In-vitro antiproliferative efficacy of Abrus precatorius seed extracts on cervical carcinoma

Kaur

Sharma

Kumar³

et al. 2022

Sci Rep

Self Cite

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“…Absorbance of mixture was measured at 725 nm. Final results were represented as quercetin equivalents (mg QE)/g of extract and tannic Acid equivalents (mg QE)/g of extract, respectively [28,30,31]. All the concentrations were calculated using a standard calibration plot.…”

Section: Quanti Cation Of Phytochemical Constituentsmentioning

confidence: 99%

“…The cells were harvested by washing with PBS followed by trypsinization. Further, the samples were centrifuged at 10,000 rpm for 20 min at 4°C to obtain the cell lysate which was further used for estimation of enzyme and non-enzyme content in accordance with standardized procedures [37].…”

Section: Antioxidant Enzyme Activity Assay On Hep2c Cellsmentioning

confidence: 99%

In Vitro Antioxidant and Antiproliferative Efficacy of Abrus precatorius Seed Extracts On Hep2c Cells and Its Inhibitory Efficacy With Receptors Of Cervical Carcinoma

Kaur¹,

Sharma²,

Waidha³

et al. 2021

Preprint

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Abrus precatorius is a widely distributed tropical medicinal plant with several therapeutic properties, however its seed extract has not been studied against cervical cancer (CaCx) till date. Herein, we have assessed the antioxidant and antiproliferative properties of A. precatorius seed extracts (ethyl acetate and 70% ethanol) prepared from different extraction methods (Soxhlet and maceration) against human cervix carcinoma cells (Hep2C). We observed a significantly higher total flavonoid content of APE (sox) i.e.,112.7±0.127 mg Quercetin Equivalent/g of extract than others; total phenolic content of APA (mac) seed extract was higher i.e.,108.53±0.089 mg Quercetin Equivalent/g of extract and total tannin content of APA (sox) was higher i.e., 98.98± 0.011 mg tannic acid equivalents/g of extract. In addition, tannic acid, rutin and piperine were identified in extracts by HPLC. Furthermore, APA (sox) exhibited the highest radical scavenging activity with an IC50 value of 14.49±0.93µg/ml. APE (mac) showed the most significant antiproliferative activity with IC50 value of 85.90±0.93µg/mL against Hep2c cells. SOD and GST activity was observed as highest in the APA(Mac) extract whereas the catalase activity and GSH content was maximum in the APE(Mac) extract. Further, the MDA content was observed to be the least in APE(Mac) extracts. Docking results suggested maximum binding energy between tannic acid and Her2 receptor compared to doxorubicin (standard). This study provides evidence that A. precatorius seed extracts possess promising bioactive compounds with probable anticancer and antioxidant properties against CaCx which might be utilized as a possible herbal remedy aimed at restricting tumor growth.

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Ocimum sanctum: Antioxidative efficacy in type 2 diabetes and its associated complications

Olofinsan,

Erukainure,

Islam

2024

Antidiabetic Medicinal Plants

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In vitro antioxidant activity of defatted seed extracts of Ocimum sanctum on rat PC-12 cells and its inhibitory efficacy with receptors of oral squamous cell carcinoma

Cited by 16 publications

References 22 publications

In-vitro antiproliferative efficacy of Abrus precatorius seed extracts on cervical carcinoma

In-vitro antiproliferative efficacy of Abrus precatorius seed extracts on cervical carcinoma

In Vitro Antioxidant and Antiproliferative Efficacy of Abrus precatorius Seed Extracts On Hep2c Cells and Its Inhibitory Efficacy With Receptors Of Cervical Carcinoma

Ocimum sanctum: Antioxidative efficacy in type 2 diabetes and its associated complications

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