2012
DOI: 10.2478/v10007-012-0026-x
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In vitro antioxidant activity of stem bark of Trichilia catigua Adr. Juss

Abstract: Antioxidant activity of the ethanolic extract and fractions from the stem bark of T. catigua was investigated. IC 50 (for DPPH scavenging) by T. catigua varied from 9.17 ± 0.63 to 76.42 ± 5.87 mg mL -1 and total phenolic content varied from 345.63 ± 41.08 to 601.27 ± 42.59 mg GAE g -1 of dry extract. Fe 2+ -induced lipid peroxidation was significantly reduced by the ethanolic extract and fractions. Mitochondrial Ca 2+ -induced dichlorofluorescein oxidation was significantly reduced by the ethanolic extract in … Show more

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Cited by 29 publications
(18 citation statements)
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“…The determination of total phenolic content was carried out as described by Kamdem JP, et al [19] . Briefly, samples of SAG extract (50-500 mg/mL) were added to a test tube and the volume was adjusted to 1.4 mL with distilled water.…”
Section: Determination Of Total Phenolmentioning
confidence: 99%
“…The determination of total phenolic content was carried out as described by Kamdem JP, et al [19] . Briefly, samples of SAG extract (50-500 mg/mL) were added to a test tube and the volume was adjusted to 1.4 mL with distilled water.…”
Section: Determination Of Total Phenolmentioning
confidence: 99%
“…Hydrolysable tannins are grouped into gallotannins and ellagitannins, which are composed of gallic acid (GLA, 3, 4, 5-trihydroxybenzoic acid) or hexahydroxydiphenic acid esters, respectively (Kraus et al 2003). The antioxidant ability of GLA provides protection against hydroxyl radical-induced deoxyribose degradation in the Fenton reaction (Kamdem et al 2012).…”
Section: Introductionmentioning
confidence: 99%
“…The antioxidant activity was determined by the ability of the aqueous and ethanolic extracts from the leaves of L. montevidensis to scavenge the DPPH radical, as described by Kamdem et al (2012[20]) with some modifications. Briefly, 50 µL of ethanolic and aqueous extracts in different concentrations (1-480 µg/mL) were mixed with 100µL of freshly prepared DPPH solution (0.3 mM in ethanol).…”
Section: Methodsmentioning
confidence: 99%