In Vitro Antioxidant and Anti-Inflammatory Activities of Bioactive Proteins and Peptides from Rhodomonas sp.

Abstract

Thanks to their various promising properties, bioactive peptides extracted from microalgae have recently attracted great attention from scientists. These compounds have been mostly obtained by enzymatic hydrolysis. In the present study, proteins from the marine microalga Rhodomonas sp. were hydrolysated into peptides using alcalase and pepsin. After obtaining the peptides, we characterized them and the crude protein by Fourier-transform infrared spectroscopy. Then, the biological activities of all protein preparations were evaluated. Antioxidant activity was investigated using DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical-scavenging and ferric reducing power assays. The obtained results showed that crude protein presents higher antioxidant activity (74% at 1 mg/mL) compared to peptides obtained after hydrolysis by pepsin (56.5% at 1 mg/mL) and alcalase (47.5% at 1 mg/mL). The anti-inflammatory activity was also evaluated by inhibition of the denaturation of albumin assay. An interesting anti-inflammatory activity was obtained using protein extract. It was improved after enzymatic hydrolysis using pepsin to reach 89% of inhibition at 500 µg/mL. The obtained data showed that the marine microalga Rhodomonas sp. could be a potential source of valuable proteins and peptides for the pharmaceutical and cosmetic fields.

Antibiofilm Efficacy and Mode of Action of Etlingera elatior Extracts Against Staphylococcus aureus

“…The breakdown of the bacterial membrane phospholipid may be shown by a decrease in the strength of the peak 1219 cm -1 (Davis & Mauer, 2010). The disappearance of spectral peaks at 1527 cm -1 (amide II) may indicate altered protein structures upon treatment with E. elatior extracts (Derbel et al, 2023). There was a spectral peak shifting at 700 cm -1 indicating the stretching vibration of C-O-C and C-O-H from glycosidic bonds.…”

Fabrication of ecofriendly silver based composite hydrogel with multifunctional properties as possible wound dressing material

“…To check the inhibition of protein denaturation all samples were placed in pre-heated water bath for 10 min at 70 °C. The mixture were cooled to room temperature and absorbance was recorded for each at 660 nm [34]. The test was performed in triplicate.…”

“…It is known that protein denaturation, which is defined as the alteration of the structure of proteins, contributes to inflammation. Consequently, it is seen as an indicator of an inflammatory disease [34,65]. Proteins that have been denatured experience the loss of their quaternary, tertiary, and secondary structures, leading to a reduction in their capacity to carry out biological processes [66].…”

“…The use of external stimuli, such as heat, or exposure to chemicals, such as organic solvents, may initiate this process. The ability of CAM composite hydrogel to prevent heat-induced albumin denaturation was tested to investigate its anti-inflammatory properties [34,67].…”

Standarisasi Ekstrak Rimpang Wundu Watu (Alpinia monopleura) dan Aktivitasnya sebagai Antiinflamasi Secara In Vitro

“…Denaturasi protein berhubungan dengan protein yang kehilangan struktur sekunder, tersier, dan struktur kuartener yang mengakibatkan hilangnya fungsi biologis. Proses ini dapat diinduksi oleh tekanan eksternal, seperti panas, atau dengan paparan bahan kimia, seperti pelarut organik (Derbel et al, 2023). Mekanisme kerja denaturasi protein yaitu menghambat pelepasan konstituen lisosom neutrofil di tempat peradangan.…”