In vitro antioxidant and antibacterial activity of sulfated polysaccharides isolated from Spatoglossum asperum

Palanisamy, Subramanian; Vinosha, Manoharan; Marudhupandi, Thangapandi; Rajasekar, Periyannan; Prabhu, Nagesh

doi:10.1016/j.carbpol.2017.04.085

Cited by 107 publications

(44 citation statements)

References 64 publications

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“…This may be due to the fact that the ethanolic extracts contain more biologically active phytochemicals than the chloroform extract. Overall, results revealed that extracts are potential source of bioactive compounds and these findings are in line with previous studies (Bajalan et al, 2017;Fakhfakh et al, 2017;Giacomini et al, 2017;Meng et al, 2017;Nair et al, 2017;Palanisamy et al, 2017;Paliwal et al, 2017;Sagbo et al, 2017).…”

Section: Antibacterial Activities Of Fruit Extractssupporting

confidence: 92%

Comparative study of the antioxidant and antibacterial activities of two guava (Psidium guajava) fruit varieties cultivated in Andasa Horticulture Site, Ethiopia

Int¹

2019

Preprint

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This investigation was carried out to evaluate the levels of phytochemicals and antioxidant potential as well as antibacterial activity of the two varieties of guava (Psidium guajava) fruit collected from Andasa horticulture site, Ethiopia. The antioxidant potential for guava fruit extracts was assessed using different in-vitro antioxidant assays, namely reducing power, Folin-Ciocalteu (F-C) assay and aluminum chloride methods. Overall, the white flesh guava fruit exhibited the highest levels of TPC, TFC and reducing power than the pink flesh guava. The mg/100g of AEAC (FRAP assay), AEAC (PMA), GAE and QE of pink and white flesh guava fruits determined in this study were 125.73, 260.28, 262.78, 377.18, 21.25, 24.61, 7.05 and 22.10, respectively. Antibacterial property of the two guava fruit variety extracts was evaluated against three gram-positive and two gram-negative bacteria using agar well diffusion technique. Ethanol extracts from both pink and white flesh guava exhibited antibacterial activity with zone of inhibition ranging from 29.50 to 30.33 and 24.33 to 29.33 mm respectively. On the other hand, only the chloroform extract from pink flesh guava showed poor antibacterial activity against MRSA and Shigella pathogens among the five tested organisms and the zone of inhibition measured was 11 and 10 mm respectively.

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Section: Antibacterial Activities Of Fruit Extractssupporting

confidence: 92%

Comparative study of the antioxidant and antibacterial activities of two guava (Psidium guajava) fruit varieties cultivated in Andasa Horticulture Site, Ethiopia

Int¹

2019

Preprint

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“…Based on previous records, the occurrence of peaks in the 1.1-1.2 ppm area is attributable to an α-L-Fucp-(1→4) connectivity pattern [16]. The proton signals between 2.0-2.2 ppm represent alcoholic protons [16], while signals between 3.5-4.0 ppm were attributed to H2-H6 sugar residues.…”

Section: Characterization Of Shc4 By Nmr and Monosaccharide Compositimentioning

confidence: 71%

Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

et al. 2020

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Ultraviolet B (UVB) radiation-induced oxidative skin cell damage is a major cause of photoaging. In the present study, a low molecular weight fucoidan fraction (SHC4) was obtained from Sargassum horneri by Celluclast-assisted extraction, followed by step gradient ethanol precipitation. The protective effect of SHC4 was investigated in human keratinocytes against UVB-induced oxidative stress. The purified fucoidan was characterized by Fourier-transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (NMR), agarose gel-based molecular weight analysis and monosaccharide composition analysis. SHC4 had a mean molecular weight of 60 kDa, with 37.43% fucose and 28.01 ± 0.50% sulfate content. The structure was mainly composed of α-L-Fucp-(1→4) linked fucose units. SHC4 treatment dose-dependently reduced intracellular reactive oxygen species (ROS) levels and increased the cell viability of UVB exposed HaCaT keratinocytes. Moreover, SHC4 dose-dependently inhibited UVB-induced apoptotic body formation, sub-G1 accumulation of cells and DNA damage. Inhibition of apoptosis was mediated via the mitochondria-mediated pathway, re-establishing the loss of mitochondrial membrane potential. The UVB protective effect of SHC4 was facilitated by enhancing intracellular antioxidant defense via nuclear factor erythroid 2–related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling. Further studies may promote the use of SHC4 as an active ingredient in cosmetics and nutricosmetics.

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“…The test tubes containing the reaction mixture was vortexed and incubated at room temperature for 30 min under dark condition. The absorbance was measured at 517 nm and l ‐ascorbic acid was used as standard (Palanisamy et al ). The percentage of radical scavenging effect was calculated as follows:

DPPH scavenging effect false(% false) = false[A_{cont} - A_{test} false/ A_{cont} false] \times 100

where A cont was absorbance of the ascorbic acid and A test was the absorbance of crude extracts.…”

Section: Methodsmentioning

confidence: 99%

“…The test tubes containing the reaction mixture was vortexed and incubated at room temperature for 30 min under dark condition. The absorbance was measured at 517 nm and L-ascorbic acid was used as standard (Palanisamy et al 2017). The percentage of radical scavenging effect was calculated as follows:…”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

Growth optimization, free radical scavenging and antibacterial potential of Chlorella sp. SRD3 extracts against clinical isolates

Santhosh

Manivannan

Ragavendran

et al. 2019

J of Applied Microbiology

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Aim The aim of present work was to explore the potential of Chlorella sp. SRD3 extracts for antioxidant and antibacterial activity along with the evaluation of minimum inhibitory concentration (MIC) and haemolytic activity to detect RBC cell damage. Methods and Results Screening and isolation of microalgae was performed using bold basal medium under normal illuminance (at 27°C) and microscopic observation. Growth of the microalgae was optimized using a different medium and light source. The isolated microalgae incubated under fluorescent light when cultured in F/2 medium showed a highest dry biomass yield of 3·77 ± 0·1 g l−1, when compared to the growth under direct sunlight (2·74 ± 0·07 g dwt l−1). The quantitative analysis of extracts revealed higher phenols, flavonoids and proanthocyanidins in ethyl acetate and hexane extracts followed by methanol. The antioxidant activity of extracts was tested against 1‐diphenyl‐2‐picrylhydrazyl and ABTS radical, its reducing power assay was performed. From antibacterial activity, the two extracts showed better inhibition against Gram‐negative bacteria. Also, they resulted in very low MIC values with effective activity against pathogens. In haemolytic activity, no haemolysis occurred, when the concentration (µg ml−1) was below 64 for methanol and 32 for ethyl acetate extract. In addition, Chlorella sp. extracts were characterized by GC‐MS analysis to detect the major compounds. Conclusion The polar extracts revealed satisfactory results against the clinical isolates and the compounds responsible were reflected in the GC‐MS spectrum. Significance and Impact of the Study The present study revealed significant biological potentials of the green alga, Chlorella sp. such as antioxidant, antibacterial and hemolytic activities. Therefore, this vital source might serve as a cost‐effective, alternative choice to the pharmaceutical and food industries in the near future.

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In vitro antioxidant and antibacterial activity of sulfated polysaccharides isolated from Spatoglossum asperum

Cited by 107 publications

References 64 publications

Comparative study of the antioxidant and antibacterial activities of two guava (Psidium guajava) fruit varieties cultivated in Andasa Horticulture Site, Ethiopia

Comparative study of the antioxidant and antibacterial activities of two guava (Psidium guajava) fruit varieties cultivated in Andasa Horticulture Site, Ethiopia

Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation

Growth optimization, free radical scavenging and antibacterial potential of Chlorella sp. SRD3 extracts against clinical isolates

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