In vitro Antioxidant and Antidiabetic activity of Silver Nanoparticles Synthesized using Catharanthus roseus leaves

Abstract

The green resources which are utilized for the synthesis of nano particles are eco-friendly and helps to evade the practice of elevated use of harmful chemicals. Bio synthesis of silver nanoparticles (AgNPs) using Catharanthus roseus leaves was carried out with their characterization including UV spectroscopy, FTIR and SEM analysis. Qualitative phytochemical analysis was done to observe the presence of phyto compounds. The antioxidant assay was done to assess DPPH and Reducing power activities. The in vitro antidiabetic assay (α-Amylase Inhibition Assay) and the in-silico molecular docking were performed to analyze the antidiabetic potential of Catharanthus leaves. Target proteins such as 11ß-hydroxysteroid dehydrogenase type I (11ß-HSD1; PDB ID: 1XU7), Glucagon like peptide-1 (GLP-1; PDB ID: 3IOL), Protein-tyrosine phosphatase 1B (PTP1B; PDB ID: 4Y14) were chosen for molecular docking against the ligands screened from GCMS data. The colour change, UV-vis spectrum, FTIR and SEM examination supported the characterization of AgNPs. The synthesized nano particles showed a strong in vitro antioxidant activity with good scavenging percentage of 90.88% compared to the standard which was 99.84% in DPPH assay. Similarly, the inhibition of α-Amylase activity increased with increase in concentration of biosynthesized nano particles. The docking study revealed that plant compounds present have the highest binding affinity and good hydrogen bond interactions with active site residues. Hence the activities demonstrated suggest that they could be useful in the preparation of many therapeutic agents.