In vitro Antioxidant and RBC membrane Stabilization Activity of Euphorbia wallichii

Tantary, Shazia; Akbar, Muhammad; Bhat, Aashiq Hussain; Dar, Khalid Bashir; Zargar, Mohammad Afzal; Ganie, Showkat Ahmad

doi:10.5530/fra.2017.1.3

Cited by 28 publications

(11 citation statements)

References 33 publications

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“…7,8 Moreover, high antioxidant product also had effect to RBC membrane stabilization. 14 Rice kefir powder showed the biochemical components including GABA, α-tocopherol and TPC higher than CMKP. Especially, HNKP had the highest GABA, α-tocopherol and TPC.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Activity, Biochemical Components and Sub-Chronic Toxicity of Different Brown Rice Kefir Powders

Chunchom¹,

Talubmook²,

Deeseenthum³

2017

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Section: Discussionmentioning

confidence: 99%

Antioxidant Activity, Biochemical Components and Sub-Chronic Toxicity of Different Brown Rice Kefir Powders

Chunchom¹,

Talubmook²,

Deeseenthum³

2017

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“…Activity: Anti-inflammatory activity was assessed using Human Red Blood Cell Stabilization assay (similar to lysosomal membrane) which determines the stability of HRBC membrane by the sample extracts to predict anti-inflammatory activity 12 .…”

Section: Determination Of In-vitro Anti-inflammatorymentioning

confidence: 99%

Untitled

2021

IJPSR

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Inflammation, activated by oxidative stress, is the leading cause of all chronic diseases. Our culinary practices should include antioxidant and anti-inflammatory foods to combat these problems. Spices in India have been used traditionally not only as flavour enhancers in food but also as natural therapeutic agents in the prevention and treatment of a wide variety of ailments owing to the presence of an array of bioactive principles. The aim of the present study was to compare the in-vitro antioxidant, antiinflammatory, and GC-MS profiles of Nigella sativa and Trigonella foenumgraecum seeds. In-vitro antioxidant and anti-inflammatory assays like DPPH (2, 2diphenyl -1-picrylhydrazyl), Phosphomolybdenum, Ferric reducing antioxidant power, Superoxide radical scavenging assay, and Human Red Blood Cell stabilization assay was conducted to analyze the antioxidant and anti-inflammatory activities. Nigella sativa seeds were observed to have more antioxidant properties than Trigonella foenum-graecum seeds in all the assays. Maximum antioxidant potential was observed for Nigella sativa seeds in superoxide radical scavenging assay with lowest IC 50 value of 17.28 µg/mL. The anti-inflammatory property was found to be higher in Trigonella foenum-graecum seeds when compared to Nigella sativa seeds. IC 50 value of Trigonella foenum-graecum seeds was found to be 54.59 µg/mL when compared to Nigella sativa seeds with an IC 50 value of 98.85 µg/mL. Since, both antioxidant and anti-inflammatory potentials are important in improving chronic diseases, both Nigella sativa and Trigonella foenum-graecum seeds should be used in sufficient amounts in our daily diet to combat diseases. INTRODUCTION:Over the decades, scientists have found that different parts of the plant contain unique therapeutic potential in curing many diseases. The growing interest in natural products is due to the side effects of the long-term use of commercially available antibiotics.

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“…This test was carried out as per the protocol described in an earlier study. 31 A fresh intravenous human blood sample was voluntarily provided by one of the contributing authors with written informed consent. The volunteer was healthy and not taking any anti-inflammatory or other medications.…”

Section: Hrbc Membrane-stabilization Assaymentioning

confidence: 99%

<p>Active <em>Cousinia thomsonii</em> Extracts Modulate Expression of Crucial Proinflammatory Mediators/Cytokines and NFκB Cascade in Lipopolysaccharide-Induced Albino Wistar Rat Model</p>

Dar

Khan

Amin

et al. 2020

JIR

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Introduction Chronic inflammation is implicated in a multitude of diseases, including arthritis, neurodegeneration, autoimmune myositis, type 2 diabetes, rheumatic disorders, spondylitis, and cancer. Therefore, strategies to explore potent anti-inflammatory regimens are pivotal from a human-health perspective. Medicinal plants represent a vast unexplored treasure trove of therapeutically active constituents with diverse pharmacological activities, including anti-inflammatory properties. Herein, we evaluated Cousinia thomsonii , an edible medicinal herb, for its anti-inflammatory/immunomodulatory properties. Methods Soxhlet extraction was used to obtain different solvent extracts (hexane, ethyl acetate, ethanol, methanol, and aqueous extract) in increasing order of polarity. In vitro anti-inflammatory assays were performed to investigate the effects of extracts on protein denaturation, proteinase activity, nitric oxide surge, and erythrocyte-membrane stabilization. The most effective extracts, ie, ethyl acetate (CTEA) and ethanol (CTE) extracts (150–200 g) were selected for further in vivo analysis using albino Wistar rats. Wistar rats received varying concentrations of CTEA and CTE (25, 50, and 100 mg/kg) for 3 weeks, followed by a single subplantar injection of lipopolysaccharide. Dexamethasone served as positive control. Blood was obtained from the retro-orbital plexus and serum separated for estimation of proinflammatory cytokines (IL6, IL1β, IFNγ and TNFα). Western blotting was performed to study expression patterns of crucial proteins implicated in the NFκB pathway, ie, NFκB p65, NFκB1 p50, and NFκB2 p52. Histopathological examination was done and gas chromatography–mass spectrometry (GC-MS) carried out to reveal the identity of compounds responsible for ameliorating effects of C. thomsonii. Results Among five tested extracts, CTEA and CTE showed marked inhibition of protein denaturation, proteinase activity, nitric oxide surge and erythrocyte-membrane hemolysis at 600 μg/mL ( P <0.001). Both these extracts showed no toxic effects up to a dose of 2,500 mg/kg. Extracts exhibited concentration-dependent reductions in expression of IL6, IL1β, IFNγ, TNFα, NFκB-p65, NFκB1, and NFκB2 ( P <0.05). Healing effects of extracts were evident from histopathological investigation. GC-MS analysis revealed the presence of important anti-inflammatory compounds, notably stigmast-5-en-3-ol, oleate, dotriacontane, ascorbic acid, n -hexadecanoic acid, and α-tocopherol, in C. thomsonii. Conclusion C. thomsonii possesses significant anti-inflammatory/immunomodulatory potential by virtue of modifying levels of proinflammatory cytokines/markers and NFκB proteins.

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In vitro Antioxidant and RBC membrane Stabilization Activity of Euphorbia wallichii

Cited by 28 publications

References 33 publications

Antioxidant Activity, Biochemical Components and Sub-Chronic Toxicity of Different Brown Rice Kefir Powders

Antioxidant Activity, Biochemical Components and Sub-Chronic Toxicity of Different Brown Rice Kefir Powders

Untitled

<p>Active <em>Cousinia thomsonii</em> Extracts Modulate Expression of Crucial Proinflammatory Mediators/Cytokines and NFκB Cascade in Lipopolysaccharide-Induced Albino Wistar Rat Model</p>

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